[PDF] Top 20 Highly Efficient Targeted Mutagenesis in Mice Using TALENs
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Highly Efficient Targeted Mutagenesis in Mice Using TALENs
... of TALENs in one-cell embryos, the coding regions can be transcribed in vitro by T7 polymerase from linearized pCAG-TALEN-pA plasmids, followed by the polyadenylation of the coding RNA with poly(A) ...polymerase. ... See full document
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Using TALENs to Knockout H2A.Lap1 Function in Mice
... whether TALENs could cause any off-target cleavage and we have successfully shown, to the best of our ability, that TALEN did not induce any off-target effects in the exonic region of our KO ...when using ... See full document
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Generation of knockout mouse models of cyclin-dependent kinase inhibitors by engineered nuclease-mediated genome editing
... of TALENs, Cas9 mRNA, and sgRNAs RNAs for TALENs and CRISPR/Cas9 were prepared as described previously ...templates using a MEGA- shortscript T7 kit (Invitrogen, USA) according to the manufacturer’s ... See full document
6
Genetic and Genomic Tools for the Marine Annelid Platynereis dumerilii
... 1996), TALENs (Cermak et ...and TALENs are both customizable DNA- binding proteins, which are fused to FokI nuclease domains and function in pairs by binding to tandem-oriented binding sites separated by ... See full document
13
TALENs Mediate Efficient and Heritable Mutation of Endogenous Genes in the Marine Annelid Platynereis dumerilii
... of TALENs for inducing targeted mutations has been reported in many conventional models, for example: mice (Davies et ...2012). TALENs are also reported to be functional in a variety of other ... See full document
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Highly efficient targeted mutagenesis in axolotl using Cas9 RNA guided nuclease
... in targeted sequences have been successfully employed to induce mutations in a variety of organisms and cell ...creating targeted indels include both synthetic targeted nucleases such as zinc-finger ... See full document
7
Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases
... Figure 6 Effects of the N and C termini of the TAL effec- tor domain on the gene-disrupting activities of deletion variants of DJ1-TALENs. (A) Schematic of deletion variants in the TAL effector domain. A natural ... See full document
14
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/Cas9-Mediated Gene Editing
... performed using infectious bacterial artificial chromosomes (BACs), which necessitates the maintenance of the viral genome in Escherichia coli and successful reconstitution of virus from permissive cells after ... See full document
10
Theranostic pH-sensitive nanoparticles for highly efficient targeted delivery of doxorubicin for breast tumor treatment
... nude mice xenografted with MCF-7 ...of mice in group 2 grew more slowly than those of group 1, which proved that DOX was an effective inhibitor of tumor ...when mice were treated with FA-DOX@IONPs ... See full document
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Apically targeted oral micelles exhibit highly efficient intestinal uptake and oral absorption
... in LP Caco-2 cells than in normal cells (Normal) and to study the PepT1-targeting efficiency of Val-PMs and Phe- PMs, qPCR analysis was applied. As shown in Figure 2, a markedly higher expression of PepT1 was found in LP ... See full document
16
Efficient and Highly Specific Gene Transfer Using Mutated Lentiviral Vectors Redirected with Bispecific Antibodies
... Adeno-associated virus (AAV) vectors remains the current gold standard in gene delivery, particularly in human patients, due to factors such as their simple binding and entry mechanisms, medium- to long-term gene ... See full document
12
Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach
... To introduce these adapted specificity- conferring codes in the A domain of Bat2mod1, the positions of the 10 AAs in Bat2mod1 that comprise the original code were identified by sequence alignments with the archetypic ... See full document
9
An efficient one step site directed deletion, insertion, single and multiple site plasmid mutagenesis protocol
... constructed using a modi- fied Gateway technology with an N-terminal TEV protease cleavable His tag ...prepared using Qiagen miniprep kits (Qiagen, Ger- ...out using a Px2 thermal cycler (Thermo ... See full document
10
Directed Evolution of Terpene Synthases for Non Natural Substrates
... When superimposed, the three models (Fig. 6) indicated the H-α1 loop region was part of the active site, which in the closed conformation allowed the magnesium ion cluster, where the diphosphate binds, to move inwards. ... See full document
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(NH4)42[MoVI72MoV60O372(CH3COO)30(H2O)72] as a Heterogeneous Reusable Catalyst for Organic Reactions: Mini-Review
... Fluoroquinolones have been a class of important of synthetic antibacterial agents which are widely used in treatment of infectious diseases [131]. These compounds act with an excellent activity against gram-negative and ... See full document
15
Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis
... Construction of C. autoethanogenum ClosTron strains. Following conjugation of ClosTron retargeting plasmids into C. autoethanogenum by using E. coli strain CA434 as the donor, thiamphenicol- and ... See full document
10
Applications and potential of genome editing in crop improvement
... generate targeted mutations in SBEIIb, leading to a higher pro- portion of long chains in amylopectin, which improved the fine structure and nutritional properties of the starch ...[43]. Using CRISPR/Cas9, ... See full document
11
Targeted DNA Mutagenesis for the Cure of Chronic Viral Infections
... As with antiviral therapy, drug resistance to specific enzymes is a potential problem. While in some cells, an enzyme may fail to cut all the available recognition sites, a more worrisome situation would occur if a ... See full document
17
Meeting report: GARNet/OpenPlant CRISPR-Cas workshop
... (DSBs) using site-specific endonucleases can enhance the freqeuncy of homologous recombination in plant cells through to his recent work using RNA-guided Cas9 nuclease to induce DSBs [12, ...directed ... See full document
7
Insertional mutagenesis in transgenic mice generated by the pronuclear microinjection procedure
... Many of the insertional mutations arising from the pronuclear microinjection technique cause interesting developmental phe- notypes. One of the first insertional mutations that causes a morphogenetic phenotype in ... See full document
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