[PDF] Top 20 Host cell-dependent alterations in envelope components of human immunodeficiency virus type 1 virions.

... and type of cell membrane adhesion molecules acquired by budding virions may have profound effects on the attachment of virions to cells, the host cell range, the binding avidity ... See full document

... target cell surface GAGs on HIV-1 infection. HIV-1 virions containing HXB and ADA HIV-1 envelopes exhibited marked and opposing differ- ences in infectivity in the presence or absence ... See full document

... per cell was sufficient to activate Ca 2⫹ signaling through ...three virions bind a CD4 ⫹ T-cell in vivo is very likely based on the range of plasma HIV RNA copies and CD4 ⫹ T-cell counts in ... See full document

... into human immunodeficiency virus type 1 (HIV-1) particles and exhibits potent antiviral activity when overexpressed in virus-producing cells ...RNA- dependent ... See full document

... CyPA-depleted virions can be restored by treatment to induce NERT (19, 20) or by pseudotyping virions with the envelope glycoprotein from ve- sicular stomatitis virus ...simian ... See full document

... different cell types has been implicated in mod- ulating HIV-1 infection (2, 39, 60, 61), we examined the endog- enous CypA level in both HeLa and Jurkat ...cells. Cell lysates were prepared from ... See full document

... medium components for cell culture were obtained from Gibco BRL Invitrogen (Carlsbad, CA) except where ...noted. Human embryonic kidney (HEK) 293 cells (American Type Culture Collection, ... See full document

... and virions were produced by transfection of 293T ...these virions were used to infect JC53BL indica- tor ...of virus are processed normally but show defects in cell-cell ...of ... See full document

... Human immunodeficiency virus type 1 (HIV-1) encodes a gp160 envelope precursor ...HIV-1 envelope gly- coprotein spikes mature by trimerization, ... See full document

... of cell-associated gp120 (Fig. 5a and c). This reduction in cell-associated gp120 was not due to a defect in gp160 processing or an increased release of gp120 into the media ...infectious virions ... See full document

... influenza virus hemagglutinin (7, 28, 47, 68) as well as HIV-1 gp160 and gp41 (13, 50, 51) have been de- tected by using polyclonal antibodies and ...by alterations to antigenic ...550-561 1 ... See full document

... from virus-expressing HeLa ...profound virus release defects in HeLa ...and virus replication in a panel of T-cell and adherent cell lines and in primary lymphocytes and ... See full document

... the host DNA, a crucial step of the retroviral cycle (see references 2, 3, and 6 for ...target cell, IN remains associated with a large nucleoprotein preintegra- tion complex (PIC), which delivers the viral ... See full document

... with virus entry may require distinct structural features in the viruses and/or recep- ...influenza virions may be essential for the cooperat- ivity that is needed for successful membrane fusion (56, ...A ... See full document

... of cell surface FIG. 5. Lentivirus transduction of the human CD4 and CXCR4 ...HIV-1 virions were then used to infect COS7 cells (A and C) or MDM (B and ... See full document

... Table 1 shows a slight change in the cell surface expression levels of CD4 and no significant changes in cell surface expression levels of CXCR4 and CCR5 following treatment of these cells with ... See full document

... significant alterations in the DRM recruit- ment of TCR machinery by Nef ...producer cell and suggested this to reflect the Nef-mediated recruitment of HIV budding structures into DRMs ... See full document

... released virions (6, 50). More recent studies have shown that vif mutant virions are blocked at a step prior to the initiation of reverse transcription, suggesting a role for Vif in the formation of ... See full document

... simian immunodeficiency virus (49, 54, 70), Mason-Pfizer monkey vi- rus (3), murine leukemia virus (47), and influenza virus (51), suggesting that sequences in the cytoplasmic domain of viral ... See full document

... the host cell are essential for Vif ...the virus-producing cell, as evidenced by the greater potency of HIV Vif proteins in human cells relative to many SIV Vif proteins (62; ...kemia ... See full document