[PDF] Top 20 Host factor-induced template modification during synthesis of poliovirus RNA in vitro.
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Host factor-induced template modification during synthesis of poliovirus RNA in vitro.
... Activity of HF-pretreated poliovirus RNA templates with reverse transcriptasea Poliovirus RNA template 805 POLIOVIRUS RNA SYNTHESIS IN VITRO Primer Mock HF pretreated 50Oi±l reaction 1.8[r] ... See full document
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Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus.
... Overall, the kinetics of poliovirus replication protein synthesis, polymerase activity, and virus production observed in the HeLa S10-initiation factor in vitro translation reactions wer[r] ... See full document
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Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell free in vitro translation RNA replication system by viral protein 3CDpro
... Standard electron microscopy processing techniques were used for negative staining. Briefly, formvar coated, 200 mesh nickel grids were prepared. Grids, sample side down were floated on droplets of suspended ... See full document
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Reversible Inhibition of Poliovirus RNA Synthesis In Vivo and In Vitro by Viral Products
... During the in vitro incorporation of [3HJUTP by viral RNA polymerase preparations in the presence of 750 x g supernatants, the following RNase inhibitors were added to the enzyme test: P[r] ... See full document
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ATP is required for initiation of poliovirus RNA synthesis in vitro: demonstration of tyrosine-phosphate linkage between in vitro-synthesized RNA and genome-linked protein.
... To examine the possibility that polyU synthesized in vitro by the poliovirus replicase is linked to a VPg-related proteins, we used anti-VPg antibodies to immunoprecipitate the labeled m[r] ... See full document
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Translating Ribosomes Inhibit Poliovirus Negative-Strand RNA Synthesis
... poliovirion RNA (4, 5, ...translation, RNA replication, and the assembly of infec- tious virus in a temporally regulated fashion ...virion RNA are added at the start of the reactions. Viral protein ... See full document
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Stimulation of Poliovirus Synthesis in a HeLa Cell-Free In Vitro Translation-RNA Replication System by Viral Protein 3CDpro
... that poliovirus RNA repli- cation in vivo requires protein translation in cis through an internal region of the genome ...the poliovirus replication com- plex in vivo requires coupled viral ... See full document
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Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
... cellular factor(s) necessary for the guanidine reversal and thus identify the role of the cellular factor(s) in the initiation of poliovirus RNA ...in poliovirus translation and ... See full document
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Identification of an RNA Hairpin in Poliovirus RNA That Serves as the Primary Template in the In Vitro Uridylylation of VPg
... acid synthesis systems are characterized first by the abortive synthesis of a short oligonucleotide attached to the hydroxyl group of a tyrosine or serine of a viral protein (ter- minal or preterminal ... See full document
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Synthesis of plus- and minus-strand RNA from poliovirion RNA template in vitro.
... The polarity of the products synthesized from plus-strand poliovirus RNA template was analyzed by Southern blotting using labeled product RNA to probe single-stranded poliovirus DNAs clo[r] ... See full document
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Anti-VPg antibody precipitation of product RNA synthesized in vitro by the poliovirus polymerase and host factor is mediated by VPg on the poliovirion RNA template.
... 715 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Antibody to the poliovirus genome-linked protein, VPg, specifically immunoprecipitated the product RNA synthesized i[r] ... See full document
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Host range phenotype induced by mutations in the internal ribosomal entry site of poliovirus RNA.
... Most poliovirus strains infect only primates. The host range (HR) of poliovirus is thought to be primarily determined by a cell surface molecule that functions as poliovirus receptor (PVR), ... See full document
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Mutation of Host Δ9 Fatty Acid Desaturase Inhibits Brome Mosaic Virus RNA Replication between Template Recognition and RNA Synthesis
... of RNA replication. Cerulenin, an inhibitor of lipid synthesis, inhibits RNA replication by poliovirus and the alphavirus SFV (16, ...lipid synthesis (33), this inhibition of RNA ... See full document
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Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro.
... The largest product RNA synthesized by the fraction V polymerase plus host factor or by the fraction III polymerase was twice the size of the poliovirion RNA template.. Dimer-sized produ[r] ... See full document
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Modification of an adenovirus major late promoter-binding factor during poliovirus infection.
... Since virus-induced modification of the UPE-USF complex parallels the in vivo inhibition of host cell RNA synthesis, the transcriptional activity of nuclear extracts from poliovirus-infe[r] ... See full document
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Inhibition of host cell transcription by poliovirus: cleavage of transcription factor CREB by poliovirus-encoded protease 3Cpro.
... of host cell RNA synthesis ...with poliovirus, for ex- ample, causes a severe decrease in transcription catalyzed by RNA polymerase I (pol I), pol II, and pol III (1, 2, ...in ... See full document
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Slow switchover from host RNA synthesis to bacteriophage RNA synthesis after infection of Escherichia coli with a T4 mutant defective in the bacteriophage T4-induced unfolding of the host nucleoid.
... Slow Switchover from Host RNA Synthesis to Bacteriophage RNA Synthesis After Infection of Escherichia coli with a T4 Mutant Defective in the Bacteriophage T4-Induced Unfolding of the Hos[r] ... See full document
11
Template-Dependent Initiation of Sindbis Virus RNA Replication In Vitro
... in vitro reactions (independent of SIN-specific replicase ...HCV RNA transcripts ...the synthesis of genome- length RNA ...initiate RNA synthesis, a transcript containing a ... See full document
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Poliovirus CRE-Dependent VPg Uridylylation Is Required for Positive-Strand RNA Synthesis but Not for Negative-Strand RNA Synthesis
... viral RNA synthesis ...preinitiation RNA replication com- plexes containing wild-type RNA templates ...RF RNA and the positive-strand RNA ...preinitiation RNA replication ... See full document
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Poliovirus 2C region functions during encapsidation of viral RNA.
... viral RNA synthesis ...inhibits RNA synthesis and causes the accu- mulation of empty capsids (6, 8, 20), contain point mutations in the 2C coding region (2, 21, 29, ...in RNA production ... See full document
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