[PDF] Top 20 Human Butyrate-Induced Transcript 1 Interacts with Hepatitis C Virus NS5A and Regulates Viral Replication

... RNA replication and particle production through the interaction with NS5A and ...HCV replication in terms of the correct folding of the replication complex required for efficient enzy- matic ... See full document

... Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) regulates viral replication through its interaction with host and other viral ...to NS5A and recruits ... See full document

... The NS5A protein has been shown to interact with several host proteins, including vesicle-associated membrane protein (VAMP)-associated protein (VAP) subtype A (VAP-A) (44) and subtype B (VAP-B) (9), FKBP8 (34), ... See full document

... promote viral infection ...(41), regulates the uncoating of the viral core after viral entry, and helps to shield viral RNA and DNA from recognition by innate immune sensors (39, ...HCV ... See full document

... epatitis C virus (HCV) is a major etiological agent of chronic liver disease, including cirrhosis and hepatocellular carci- noma ...enveloped virus with a positive-sense, single-stranded RNA that ... See full document

... and viral proteins involved in HCV RNA replication (19, 49, ...RNA replication (12). In addition, Evans et al. suggested that NS5A hyperphosphorylation dis- rupts interaction with VAP-A and ... See full document

... for virus assembly ...Several viral proteins of other viruses have been reported to interact with the cytoskel- eton or cytoskeleton-associated ...encephalitis virus and Kunjin virus, which, ... See full document

... ligase c-Cbl (68). Another study also indicated that vinexin ␤ regulates the proteasome-dependent deg- radation of WAVE2 in a protein kinase A-dependent manner ...of NS5A phosphorylation in- duced by ... See full document

... While the present work was in review, Lyn et al. (49) reported that inhibition of SCD1 disrupted the integrity of membranous HCV replication complexes and rendered HCV RNA susceptible to nuclease-mediated ... See full document

... HCV replication. Hsp90 regulates the folding and stability of proteins in all eukaryotes (59), and inhibition of the chaperone pathway suppresses correct protein folding, which leads to induction of ... See full document

... of NS5A with YB-1 and ...YB-1 regulates the HCV life cycle, we attempted to identify novel interactions between HCV pro- teins and ...and NS5A have notably been shown to have a larger ... See full document

... (Table 1) from selected colonies that we could grow from our lethal or near-lethal mutant replicons (C1985E, T1989L, D1990K, K1992D, and D1979K/D1982K), but the only second-site re- version that functionally ... See full document

... of hepatitis C virus (HCV) on the antigenic properties of the NS5 protein was studied by using recombinant ...HCV NS5A protein at amino acids 2212 to ...genotypes 1 through ...the ... See full document

... in hepatitis C virus genome ...the viral positive strand genomic ...inhibit hepatitis C virus (HCV) ...RNA replication, was unaffected by ...HCV replication ... See full document

... sequence. Virus names and respective NCBI protein identifications or GenBank accession numbers: HCV-Con 1, HCV genotype 1b sequence (AJ238799); HCV-H77, HCV infectious clone 1a H77 (AF009606); GBV-B, ... See full document

... A protein band with a molecular mass of around 72 kDa was pulled down by the biotin-UTR-Luc RNA (Fig. 1A). LC- MS/MS data indicated that 11 of the identified peptides had an amino acid sequence matching different regions ... See full document

... infectious virus in the supernatant of transfected cells were then determined on cells expressing no, wild-type, or p3-4 ...miRNA-independent replication can occur ...wild-type virus made in cells ... See full document

... within NS5A domains II and III that are dispens- able or required for HCV RNA replication in the context of a genotype 1b GIT ...important replication elements flanking these regions or the ... See full document

... Regulated cleavage at the NS4B-5A junction determines DMV biogenesis. For several RNA viruses, it has been shown that processing intermediates serve distinct functions in the replica- tion cycle. In HCV, polyprotein ... See full document

... of NS5A translated ...RNA replication and proved not to affect translation activity after mutating the HCV 5 ⬘ UTR ...HCV replication and translation can be independently as- ... See full document