[PDF] Top 20 Human ESCRT-II Complex and Its Role in Human Immunodeficiency Virus Type 1 Release

... cerevisiae ESCRT-II complexes are func- tional ...between human and yeast ESCRT-II ...mammalian complex lacks both NZF domains and therefore must interact differently with both ... See full document

... of role of p6 in virus production in diverse cell ...efficient virus release in HeLa cells. We have also demonstrated that virus replication in most T- cell lines and in PBMC and MDM is ... See full document

... MHC II is likely to result in reduced antigen presentation to T ...MHC II expression in virus-infected ...MHC II), either without permeabilization for surface levels or after permeabilization ... See full document

... several factors, including those which directly affect the activity of the RT enzyme (i.e., divalent cations), the NC protein, the configuration of the RNA or DNA template, and the spatial organization of these ... See full document

... ces II and III constitute the dimer interface in the majority of the ...major role in trafficking the Vpr to the nuclear rim ...helices II and III, while oligomerization includes helix I ...proposed ... See full document

... Tissue culture, DNA transfection, and virus infection. The SupT1 T-cell line was grown in RPMI 1640 medium containing 10% fetal calf serum (FCS) at 37 8 C FIG. 1. Models for HIV-1 RNA dimerization ... See full document

... A role for ADAM10 during nuclear trafficking is in concert with known cellular roles for this complex pro- ...to its interac- tion with the malaria PfSUB2 enzyme ...proteolytic release of many ... See full document

... proteinase-treated virus was de- termined in three ways: (i) RT assay of culture supernatants (46) and (ii) p24 antigen assay, both of which assessed produc- tion of new virions 7 to 9 days after infection; ... See full document

... Most replication-defective gag mutants exhibit altered core morphology with round rather than cone-shaped cores (16, 21, 31, 46). Wild-type virion preparations also contain a large fraction of virions with round ... See full document

... the role of MA in HIV-1 repli- cation and to map functional domains within this protein, we have previously used site-directed mutagenesis to introduce over 80 single and double amino acid substitutions in ... See full document

... GPI-anchored proteins preferentially associate with the lipid raft microdomains of the plasma membrane and as a conse- quence decorate the surface of outgoing HIV-1 particles (40). The removal of membrane ... See full document

... the human immunodeficiency virus type 1 (HIV-1) nucleoprotein complex with the cell nuclear import machinery is necessary for viral replication in macrophages and for the ... See full document

... Our results indicate that establishment of a persistent infec- tion by HIV-1 differs from that of less complex retroviruses. Establishment of infection typically results after integration of the viral DNA. ... See full document

... and its safety profile is comparable to placebo with the exception of ...grade 1 or 2 in the great majority of patients and was ...drug; its safety, pharmacokinetic profile, and efficacy have not ... See full document

... a complex with high-molecular- weight proteins that we have identified as CC2D1A and ...in human cells but not with any other ESCRT-III com- ...the ESCRT pathway is further implied by the ... See full document

... the ESCRT machinery is ...dominant-negative ESCRT-III component. As shown in Fig. 3 (lanes 1 and 2), CHMP3-RFP had little if any effect on the release of Z IL -p6 Gag under conditions in which ... See full document

... HIV-1 release was ESCRT-II-independent since the depletion of the ESCRT-II subunit EAP20 by small interfering RNA did not appear to cause a significant effect on viral ... See full document

... a human cell line lacking N- acetylglucosamine transferase I (GnTI), the enzyme that initiates the conversion of oligomannose N-glycans into complex ...Thus, complex glycans, including those that ... See full document

... modulate virus infectiv- ...of virus assembly by enabling the establishment of integrated provirus in new target ...a complex during virus ...purified human immunodeficiency ... See full document

... wild-type virus production in macrophage cultures was always approxi- mately 10 ng of p24 per ml over a 100-fold range of multiplic- ities of ...the virus yield approximated the input inoculum (20 ng ... See full document