[PDF] Top 20 Human Immunodeficiency Virus Type 2 Vpx-Gag Interaction
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Human Immunodeficiency Virus Type 2 Vpx-Gag Interaction
... the Gag proteins (4, 7), although it is dispens- able for the assembly process ...with Gag could mediate the packag- ing of Vpx. In addition, HIV-2 Vpx appears to colocalize with ... See full document
5
Hsp40 Facilitates Nuclear Import of the Human Immunodeficiency Virus Type 2 Vpx-Mediated Preintegration Complex
... of Vpx caused by overexpression of Hsp40/DnaJB6 (H31Q) might be the consequence of the al- tered assembly-disassembly dynamics of the cytoskeleton net- work as a result of Hsp70 ... See full document
9
Gag-Specific CD4+ T-Cell Frequency Is Inversely Correlated with Proviral Load and Directly Correlated with Immune Activation in Infection with Human Immunodeficiency Virus Type 2 (HIV-2) but Not HIV-1
... were resuspended in 1 ml of complete medium (29) with 1 g/ml anti-CD28 (BD Biosciences, San Jose, CA) and cultured for 6 h alone or together with the HIV-1 or HIV-2 Gag peptide pools with the addition of ... See full document
5
Tsg101, an Inactive Homologue of Ubiquitin Ligase E2, Interacts Specifically with Human Immunodeficiency Virus Type 2 Gag Polyprotein and Results in Increased Levels of Ubiquitinated Gag
... HIV-1 Gag and Tsg101 (13, 31, ...HIV-2 Gag is required for interaction with ...p6 Gag domains of all known primate lentiviruses, it is not surprising that both HIV-1 and HIV-2 ... See full document
10
Analysis of Human Immunodeficiency Virus Type 1 Gag Dimerization-Induced Assembly
... precursor Gag (PrGag) pro- teins of retroviruses such as human immunodeficiency virus (HIV), Rous sarcoma virus (RSV), and murine leukemia vi- ruses are sufficient for the production of ... See full document
9
A highly divergent simian immunodeficiency virus (SIVstm) recovered from stored stump-tailed macaque tissues.
... Nucleotide sequence analysis of the gag and env regions indicates that SIVstm is an ancient member of the SIV/human immunodeficiency virus type 2 group; it is quite divergent from known [r] ... See full document
5
Defect of Human Immunodeficiency Virus Type 2 Gag Assembly in Saccharomyces cerevisiae
... HIV-2 Gag dissociates from yeast membrane in the absence of ...of Gag, mem- brane flotation experiments were carried out using sucrose step ...HIV-1 Gag-Flag were analyzed, Gag was ... See full document
11
A Single Amino Acid Substitution in a Segment of the CA Protein within Gag That Has Similarity to Human Immunodeficiency Virus Type 1 Blocks Infectivity of a Human Endogenous Retrovirus K Provirus in the Human Genome
... pCRU5-HERV-K gag expression vector, 2 g or 10 g was transfected into 293T cells using Opti-Mem and Lipofectamine 2000 (Invitrogen), and particle production was assessed by West- ern ...HERV-K Gag ... See full document
10
Reversal of Human Immunodeficiency Virus Type 1 Protein-Induced Inhibition of Natural Killer Cell Activity by Alpha Interferon and Interleukin-2
... Env- Gag recombinant HIV fusion protein expressed in Escherichia coli and used in this investigation have been described previously (6, 8, ...and gag regions of the HIV-1 genome. The sequence of ... See full document
5
Analysis of Human Immunodeficiency Virus Type 1 Gag Ubiquitination
... than Gag might promote Gag ...in Gag ubiquitination were not due to different amounts of immunoprecipitated Gag ...ubiquitinated Gag cleavage products in the mature ...mature ... See full document
11
Assembly Properties of Human Immunodeficiency Virus Type 1 Gag-Leucine Zipper Chimeras: Implications for Retrovirus Assembly
... the Gag-Z pro- teins may actually be identical to that of WT ...The Gag-Z VLPs are roughly the same size and shape as WT VLPs and thus are roughly the same ...their Gag-Z pro- teins ...each ... See full document
10
Novel Gag-Pol Frameshift Site in Human Immunodeficiency Virus Type 1 Variants Resistant to Protease Inhibitors
... FIG. 3. Construction and analysis of HIV frameshift mutant molecular clones. (A) A 930-bp DNA fragment containing part of the p7 gene, the entire p1 gene, and 39 sequences leading into the reverse transcriptase gene from ... See full document
5
Induction of Neutralizing Antibodies and Gag-Specific Cellular Immune Responses to an R5 Primary Isolate of Human Immunodeficiency Virus Type 1 in Rhesus Macaques
... explanted human infant lung tissue (21), JR-FL was isolated from frontal lobe brain tissue of a patient with AIDS dementia (53), and P15 and P27 were isolated during early seroconversion by PBMC coculture ...All ... See full document
12
Linker insertion mutations in the human immunodeficiency virus type 1 gag gene: effects on virion particle assembly, release, and infectivity.
... , filtered supernatants from transfected COS7 cells were pelleted over a 25 to 45% sucrose step gradient for 16 h at 100,000 3 g. The 25%-45% interface was harvested, diluted fivefold with TNE, and repelleted for ... See full document
9
Cyclosporine A-resistant human immunodeficiency virus type 1 mutants demonstrate that Gag encodes the functional target of cyclophilin A.
... the interaction between CyPA and HIV-1 Gag and inhibits HIV-1 replication (28, ...of Gag were associated with drug resistance; analysis of the primary structure of this region revealed that all ... See full document
7
A Putative α-Helical Structure Which Overlaps the Capsid-p2 Boundary in the Human Immunodeficiency Virus Type 1 Gag Precursor Is Crucial for Viral Particle Assembly
... Nuclear magnetic resonance and crystal structures show that the N-terminal domain of CA is largely a-helical (18, 35). The PHD program (45–47) predicts that the C-terminal assembly domain of CA contains four a-helices ... See full document
7
Inhibitors of human immunodeficiency virus type 1 zinc fingers prevent normal processing of gag precursors and result in the release of noninfectious virus particles.
... prevent virus from binding to ...prevent Gag precursor processing and maturation in the man- ner of protease inhibitors, without any evidence for a signifi- cant inhibition of the protease ... See full document
10
Distinct Intracellular Trafficking of Equine Infectious Anemia Virus and Human Immunodeficiency Virus Type 1 Gag during Viral Assembly and Budding Revealed by Bimolecular Fluorescence Complementation Assays
... EIAV Gag assembly by BiFC ...EIAV Gag assembly by confocal microscopy in EIAV-permissive ED cells (Fig. 2, pan- els a to c) and in HeLa cells ...(Fig. 2, panels d to f). Both ED (Fig. ... See full document
10
Human immunodeficiency virus type 1 glycoprotein precursor retains a CD4-p56lck complex in the endoplasmic reticulum.
... Intracellular interaction of human immunodeficiency virus type 1 ARV-2 envelope glycoprotein gpl60 with CD4 blocks the movement and maturation of CD4 to the plasma membrane.. Intracellul[r] ... See full document
6
Cytoplasmic Utilization of Human Immunodeficiency Virus Type 1 Genomic RNA Is Not Dependent on a Nuclear Interaction with Gag
... sarcoma virus and prototype foamy virus, Gag proteins are known to shuttle between the nu- cleus and the cytoplasm and are implicated in nuclear export of the viral genomic unspliced RNA (gRNA) for ... See full document
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