[PDF] Top 20 Human Papillomavirus DNA Replication Compartments in a Transient DNA Replication System

... sented DNA that was not taken up by the cells or transported into the ...ori DNA and that most of the unrepli- cated, intranuclear ori DNA was quickly degraded to below the level of ... See full document

... overall replication scheme appears similar to its counterpart in alphaherpesviruses (62), the lytic replication process of HCMV is likely to be more complex for several reasons: (i) HCMV oriLyt spans more ... See full document

... probe, DNA bands corresponding to the plasmids used for transient replication assays were recorded on X-ray films, using anti-DIG-AP and CDP-Star according to the accompanying protocols (Roche ... See full document

... vitro replication of HPV-16 DNA. Transient replication assays have demonstrated that interaction between HPV-16 E1 and E2 is required for efficient origin-dependent papillomavi- rus ... See full document

... the papillomavirus ampli- ficational replication characterized in the model system studied is actually used by the virus to control the productive infection of basal ...low-risk human and ... See full document

... ori DNA-binding domains, E1 comprises a more divergent N-terminal region that is strictly required for DNA replication in ...in replication of the viral ...viral DNA replication ... See full document

... sient DNA replication, it greatly enhances the overall efficiency of the ...reduce transient DNA replication by 70 to 75% but never com- pletely abolish ...in transient ... See full document

... vitro DNA replication with human cell ...polyomavirus DNA, PV DNA can be replicated in many different cell types when the viral E1 and E2 proteins are provided ...vitro DNA ... See full document

... model system for studying papillomaviruses has been bo- vine papillomavirus type 1 (BPV1) because of its ability to in- fect and transform a variety of rodent cells in tissue culture ...and ... See full document

... the transfected population (Fig. 2B, column 2). Interest- ingly, E2m1 and E2m2 fail to induce apoptosis in these cells (columns 3 and 4). In contrast, the remaining mutants induce apoptosis to levels comparable to that ... See full document

... LMW DNA was extracted from U2OS cells transfected with wild-type HPV18 and HPV18E8 ⫺ DNA 5 days after electroporation via Hirt ...LMW DNA were treated with the indi- cated concentrations of the BglI ... See full document

... 48-h-p.t. DNA samples were digested with XhoI to linearize the plasmid and with DpnI to remove input ...efficient replication of HPVOri. This HPVOri replication was dependent on E2, as cells ... See full document

... viral replication factories (16); we therefore evaluated the effect of Rint1 overexpression on viral DNA ...E1/E2 replication foci is E2 ...Cellular DNA was stained with Hoechst 33342 ... See full document

... bovine papillomavirus type 1 (BPV-1) E2 protein is the master regulator of papillomavirus replication and ...C-terminal DNA-binding and dimerization ...the replication- and ... See full document

... Among all the mutants tested the helicase activity of R505E is surprising since it is impaired in all activities, including ssDNA binding, required for unwinding. Further- more, high protein concentrations did not ... See full document

... Ad.2 DNA replication when compared to plasmids containing only the 1-18 core origin of replication (Hay et ...vitro replication studies by Mul et ...stimulate replication has been ... See full document

... Prelabeled, depleted nuclei were incubated at two concentrations of nuclei with increasing amounts of cytoplasmic protein extracts from polyoma-infected cells microliters.. Incubations w[r] ... See full document

... Percentage of nuclei that incorporated [3H]TdR into cellular DNA and exhibited CMVspecific fluorescence after infection" % Nuclei incorporating [3H]TdR in: CMV-infected Mock-infected Pre[r] ... See full document

... of DNA synthesis after UV irradiation that contribute to [³H]thymidine incorporation: DnaA dependent initiation of replication at oriC, DnaA independent UV induced synthesis (iSDR) at other chromosomal ... See full document

... HPV-16 DNA replication by using a combi- nation of neutral/neutral and neutral/alkaline two-dimensional gel ...of DNA replication was mapped by the neutral/neutral two-di- mensional gel method ... See full document