[PDF] Top 20 Identification and analysis of a lytic-phase origin of DNA replication in human herpesvirus 7.
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Identification and analysis of a lytic-phase origin of DNA replication in human herpesvirus 7.
... plasmid DNA by electroporation, and replication assays were performed as described previously ...and DNA fragments were resolved on an agarose ...this DNA with a radiolabeled pKS probe. (1) ... See full document
6
Human Immunodeficiency Virus Replication in a Primary Effusion Lymphoma Cell Line Stimulates Lytic-Phase Replication of Kaposi’s Sarcoma-Associated Herpesvirus
... KSHV replication and transmission by antiretroviral therapy may have relevance to recent clinical reports of KS regression upon treatment with protease inhibitor-containing regimens (7, 10, 30, ...(Fig. ... See full document
10
Identification of the lytic origin of DNA replication in human cytomegalovirus by a novel approach utilizing ganciclovir-induced chain termination.
... For quantitation of this type of analysis, we studied the total intracellular viral DNA from DHPG-treated cultures by using dot blot hybridization, and we compared the levels of accumula[r] ... See full document
12
Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Contains Two Functional Lytic Origins of DNA Replication
... virus lytic cycle, is sufficient to replicate the cloned HHV-8 ...cellular DNA was harvested 2 or 4 days posttransfection. Replication products were detected in samples where cells were treated with ... See full document
7
Genome Sequence of Bovine Herpesvirus 4, a Bovine Rhadinovirus, and Identification of an Origin of DNA Replication
... Notes on further genes. Until now, RNAs of six genes of BoHV-4 were demonstrated experimentally to be spliced. Be- sides Bo5, Bo10, and Bo11, these are ORFs 29, 50, and 57 (7, 44). ORF 29 encodes terminase, a ... See full document
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Activation of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Lytic Replication by Human Cytomegalovirus
... KSHV lytic cycle proteins, ...KSHV lytic ORF 59 ...2. Analysis of ORF73/LANA and viral DNA in long-term ...with phase contrast (vis) and fluorescence (uv) ...gel analysis of ... See full document
9
Kaposi's Sarcoma-Associated Herpesvirus Lytic Origin (ori-Lyt)-Dependent DNA Replication: Identification of the ori-Lyt and Association of K8 bZip Protein with the Origin
... for lytic DNA replication as well as K8 ...cellular DNA replication origins, and ini- tiation of replication generally involves local unwinding at an AT-palindromic sequence ... See full document
11
Identification of Persistent RNA-DNA Hybrid Structures within the Origin of Replication of Human Cytomegalovirus
... the origin of replication of human herpesvirus 6 (HHV-6) ...the origin may be the result of the acquisition of oriLyt by transposition ...same DNA strand as ...regions. ... See full document
8
Role of ORF74-Encoded Viral G Protein-Coupled Receptor in Human Herpesvirus 8 Lytic Replication
... the identification of ORF74 deletion ...reactivated lytic replication of bacmid genomes. (A) Initial analysis of ORF74- deleted ( ⌬ ) and -repaired ( ⌬ R; reverted to wild type [wt]) genomes ... See full document
6
Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 K-bZIP Modulates Latency-Associated Nuclear Protein-Mediated Suppression of Lytic Origin-Dependent DNA Synthesis
... the replication assay. We also wanted to examine latent and lytic replication simulta- neously since, in the context of viral infection, both LANA and lytic replication factors are ... See full document
10
Sequence Requirements for Interaction of Human Herpesvirus 7 Origin Binding Protein with the Origin of Lytic Replication
... ment. Replication efficiency is enhanced by adjacent sequences that include a GC-rich segment and a putative DNA unwinding element within the AT-rich imperfect direct repeats (IDRs) of 187 and 192 bp ... See full document
12
Identification of the Rhesus Macaque Rhadinovirus Lytic Origin of DNA Replication
... of human herpesvirus 8 (HHV-8), or Ka- posi’s sarcoma-associated herpesvirus, was isolated from a sim- ian immunodeficiency virus-infected animal with a lymphopro- liferative disorder (25, ...a ... See full document
7
Detection of Human Herpesvirus 7 DNA by Loop Mediated Isothermal Amplification
... viral DNA in peripheral blood mononuclear cells by sensitive PCR may not be able to differentiate between active and latent infections in organ transplant ...viral DNA. This result suggests that ... See full document
5
Replication Origin of Mitochondrial DNA in Insects
... 200 m m of each dNTP, 5 pmol of the M13F20 primer, 5 pmol of primer 2, 0.5 ml of the purified primer extension product, and 1.88 units of Taq DNA polymerase. The reaction was carried out for 30 sec at 95°, for 1 ... See full document
11
Replication of herpesvirus DNA. III. Rate of DNA elongation.
... One approach to determining the rate of replication of viral DNA molecules is to ascertain the buoyant density of the newly synthesized viral DNA labeled with [3H]BUdR for various period[r] ... See full document
8
Identification of a subset of herpesvirus saimiri polypeptides synthesized in the absence of virus DNA replication.
... The synthesis of most of the major virus-induced polypeptides was detected between 24 and 31 h, at which time rates of virus DNA synthesis were maximal in control cultures; of these poly[r] ... See full document
5
Herpesvirus Lytic Replication and the Cell Cycle: Arresting New Developments
... In contrast to the studies by Ehmann et al. (17) and Song et al. (41) showing that HSV infection suppresses cyclin-cdk func- tion, a study by Hossain et al (22) reported that infection of serum-starved CV-1 cells results ... See full document
7
Identification of critical elements within the JC virus DNA replication origin.
... The only origin plasmid that contained SV40 late sequences and exhibited detectable replication activity in POJ-19 cells was pJLo, a construct in which all but 22 nucleotides of the SV40[r] ... See full document
11
Isolation and Identification of Human Herpesvirus 7 from an Infant with Exanthem Subitum
... Isolation and Identification of Human Herpesvirus 7 from An Infant with Exanthem Subitum Isolation and Identification of Human Her~esvirus 7rom An Infant with Exanthem Sub~tum K B Chua, MRCP*; S K Lam[.] ... See full document
6
Identification of cellular factors that bind specifically to the Epstein-Barr virus origin of DNA replication.
... To identify cellular proteins possibly involved in EBV plasmid maintenance, protein-DNA gel retardation experiments and DNase I footprinting were performed, * using HeLa cell nuclear ext[r] ... See full document
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