[PDF] Top 20 Identification of GBF1 as a Cellular Factor Required for Hepatitis C Virus RNA Replication

... BFA-sensitive factor required for HCV replica- ...viral factor. The best-documented cellular targets of BFA are members of the ARF ...exchange factor 1 (BIG1), BIG2, or Golgi BFA ... See full document

... be the main site for adaptation by Blight et al. (7). Although they demonstrate a lack of correlation between hyperphospho- rylation and adaptation, and this was also found by our group (N. Krieger and R. Bartenschlager, ... See full document

... proteins that contain various phosphorylated serine/threonine- proline motifs; thus, both proteins could be potential sub- strates of PPIases. We first examined this possibility by in vitro GST pulldown assays using GST ... See full document

... and C terminal (TC1 to TC144), point mutations in the four conserved motifs of radical SAM enzymes (M1 to M4), a leucine zip motif (LZ5), and Cys313 (C313A) and Trp361 at the C terminus (W361A, W361D, ... See full document

... epatitis C virus (HCV) is a major cause of chronic hepatitis, cirrhosis, and hepatocellular ...positive-strand RNA virus classified within the family Fla- ...HCV RNA genome is ... See full document

... be required for the morpho- genesis and secretion of HCV (for a recent review, see refer- ence ...HCV RNA replication and the levels of infectious viral ...total cellular RNA was ... See full document

... Epstein-Barr virus replication origin region (OriP) (8, ...viral RNA could be detected transiently (16). Additionally, HCV replication in the culture cells could be enhanced by expression of ... See full document

... the cellular interactome of the viral transcription/replication machin- ...A virus encoding a Strep-tag at the C terminus of the PB2 subunit of the viral RdRP to isolate protein complexes ... See full document

... or virus-induced IRF3 activation (17), suggesting that it is required for RIG signaling to ...HCV RNA replication ...of cellular permissiveness for HCV RNA replica- tion, ... See full document

... the cellular and viral ...teins required for the production of infectious virus par- ticles, their secretion and ...for replication of HCV positive and negative strand RNA ...NS5b ... See full document

... of replication-defective NS5A ...and C [Table 1]), each of which is potentially associated with a distinct RNA replication function ...binding, RNA binding, and NS5A dimerization (7, ... See full document

... increase replication efficiency ...the replication efficiency (15; Blight and Rice, unpublished re- sults), engineering these NS3 changes into SG-5⬘HE (S2204I) did not enhance replication in our ... See full document

... H77 RNA synthesis in Huh- ...dengue virus, NS4B interacts with NS3, resulting in the displacement of NS3 from single-stranded RNA ...supporting RNA replication, where all the compo- ... See full document

... and C). These include fairly dramatic increases in replication fitness in S2204I, such as in the case of C3-4 (M313A), E1-2 (P324A), and to a lesser extent F2-1 ...for replication in the context of ... See full document

... Hepatitis C virus (HCV) infection is the leading cause of liver cirrhosis and hepatocellular carcinoma and one of the primary indications for liver ...HCV replication remain poorly defined. ... See full document

... the identification of adaptive mutations in the HCV subgenomic replicon, it has been noted by several groups that the hyperphosphorylated form of NS5A, p58, is not necessary for replication in cell ...HCV ... See full document

... integrity, virus infection, and preventing the development of cancer. Hepatitis C virus (HCV) infection and the expression of the HCV nonstructural protein NS3 and core protein have been ... See full document

... HCV RNA in these ...HCV-O RNA was notably suppressed in DDX3 knockdown O cells ...HCV RNA from O cells by interferon treatment) transfected with the genome- length HCV-O RNA with an adapted ... See full document

... of GBF1 with NS3 is consistent with the crucial role of GBF1 at the onset of genome replication, since the NS3-4A protease mediates one of the earliest steps of HCV replication, the ... See full document

... 2 RNA replication, which is in contrast to what was found with genotype 1 replicons (10, ...11). Replication of mutants SL-IIz ⬘ - 56-59, SL-IIz ⬘ -78-82, and SL-IIz ⬘ -91-97 was very similar to that ... See full document