[PDF] Top 20 Identification of an ICP27-Responsive Element in the Coding Region of a Herpes Simplex Virus Type 1 Late Gene
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Identification of an ICP27-Responsive Element in the Coding Region of a Herpes Simplex Virus Type 1 Late Gene
... with ICP27 expression plasmid pC27 (27), except when the plasmid pM27 (34) was used to express ICP27 (see ...simian virus 40 (SV40) late poly(A) region, as well as a deletion of much of ... See full document
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Identification of an Export Control Sequence and a Requirement for the KH Domains in ICP27 from Herpes Simplex Virus Type 1
... block ICP27 from exiting the ...that ICP27 carrying mutations in the KH1 domain can still bind to and export some of its cargo ...of ICP27 and cause a complete loss of ICP27-dependent ... See full document
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Arginine-rich regions succeeding the nuclear localization region of the herpes simplex virus type 1 regulatory protein ICP27 are required for efficient nuclear localization and late gene expression.
... of ICP27, with the BamHI site designated nucleotide (nt) 1 and the SstI site designated nt 2421 as previously described (23), were digested at the BamHI site and the EcoRI site which occurs in the PEFR ... See full document
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Herpes Simplex Virus Type 1 Gene UL14: Phenotype of a Null Mutant and Identification of the Encoded Protein
... The herpes simplex virus type 1 (HSV-1) genome contains 74 different known protein-coding genes (18, ...UL14 coding region is relatively small (219 codons), ... See full document
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Shuttling of the herpes simplex virus type 1 regulatory protein ICP27 between the nucleus and cytoplasm mediates the expression of late proteins.
... in ICP27 (25) and the ability of ICP27 to shuttle at late times postinfection suggested that nuclear export of this protein might be dependent on the presence of virus-specified late ... See full document
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Identification of nuclear and nucleolar localization signals in the herpes simplex virus regulatory protein ICP27.
... which ICP27 carries out its lytic functions have not yet been ...that ICP27 may work, at least in part, through posttranscriptional gene regulatory ...which ICP27 can either activate or ... See full document
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Neurons Differentially Activate the Herpes Simplex Virus Type 1 Immediate-Early Gene ICP0 and ICP27 Promoters in Transgenic Mice
... Herpes simplex virus type 1 (HSV-1) immediate-early (IE) proteins are required for the expression of viral early and late ...IE gene promoters (ICP0 and ... See full document
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Identification of Sequences in Herpes Simplex Virus Type 1 ICP22 That Influence RNA Polymerase II Modification and Viral Late Gene Expression
... The mechanism by which ICP22 enhances viral gene expres- sion is not yet known. At least two models have been put forth. First, it has been proposed that ICP22 enhances L-gene ex- pression by activating the ... See full document
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The Initiator Element in a Herpes Simplex Virus Type 1 Late-Gene Promoter Enhances Activation by ICP4, Resulting in Abundant Late-Gene Expression
... during infection, four mutants were constructed in the gC locus of KOS. One of the mutants, gC-Inr*, contains the LS8 muta- tion and is otherwise intact with respect to the remainder of the gC locus. The viruses gC-DPE* ... See full document
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Intracellular localization of the herpes simplex virus type 1 major transcriptional regulatory protein, ICP4, is affected by ICP27.
... of herpes simplex virus (HSV) gene expression during productive ...synergistic. ICP27, which alone does not exhibit major transregulatory activity, is able to differentially activate ... See full document
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Herpes simplex virus type 1 ICP27 deletion mutants exhibit altered patterns of transcription and are DNA deficient.
... From these studies it can be concluded that the 1.2-kb deletion in 3d11.2 and 5d11.2 results simultaneously in their inability i to induce the synthesis of detectable ICP27, ii to down-r[r] ... See full document
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DNA binding and gene regulation by the herpes simplex virus type 1 protein ICP4 and involvement of the TATA element.
... The regions of the herpes simplex virus type 1 immediate early protein Vmw 175 required for site specific DNA binding closely correspond to those involved in transcriptional regulation..[r] ... See full document
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Identification of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.
... 818 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Hybrid arrest of in vitro translation was used to localize the region of the herpes simplex virus type 1 genome enco[r] ... See full document
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Extensive homology between the herpes simplex virus type 2 glycoprotein F gene and the herpes simplex virus type 1 glycoprotein C gene.
... Analysis of the potential coding sequences within the 2.9-kb Sall DNA fragment of HSV-2 revealed an open reading frame of 479 amino acids which began with the methionine encoded at posit[r] ... See full document
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Mutational analysis of two herpes simplex virus type 1 late promoters.
... A TATA element is the only consensus sequence element identified in the upstream regulatory region of late viral promoters 18, and deletion studies have indicated that little sequence in[r] ... See full document
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Expression of the herpes simplex virus type 1 glycoprotein C gene requires sequences in the 5' noncoding region of the gene.
... In previous studies using recombinant viruses, we demonstrated that sequences between -1350 and +30 relative to the start of gC transcription regulate 13-Gal as a viral late gene and tha[r] ... See full document
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Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2
... Immunoprecipitation. At the end of the chase, the cells were quickly chilled by being placed on ice and treated with ice-cold PBS. Immunoprecipitation was performed under denaturing conditions. In brief, the radiolabeled ... See full document
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Herpes simplex virus type 1-specific cytotoxic T lymphocytes recognize immediate-early protein ICP27.
... While all mice strains tested C3H/HeN [H-2k], C57BL/6 [H-2b], and BALB/c [H-2d] demonstrated a vigorous CTL response specific for HSV-1 syngeneic target cells infected with HSV-1 demonst[r] ... See full document
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The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation
... Herpes simplex virus specifies two sets of transcripts from the UL24 gene, short transcripts ...and late kinetics, re- ...viral ICP27 mutants, we investigated whether ... See full document
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Processing of α-Globin and ICP0 mRNA in Cells Infected with Herpes Simplex Virus Type 1 ICP27 Mutants
... the ICP27 gene are shown in Fig. 1. Mutants d27-1, n59R, n263R, n406R, and n504R (42, 45), d1-2 (44), d3-4 and d4-5 (31), and M11, M15, and M16 (43) have been described ...the ICP27 ... See full document
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