[PDF] Top 20 Indeterminate tcdB using a Clostridium difficile PCR assay: a retrospective cohort study
Has 10000 "Indeterminate tcdB using a Clostridium difficile PCR assay: a retrospective cohort study" found on our website. Below are the top 20 most common "Indeterminate tcdB using a Clostridium difficile PCR assay: a retrospective cohort study".
Indeterminate tcdB using a Clostridium difficile PCR assay: a retrospective cohort study
... This study has several important limitations including its small sample size and retrospective .... difficile testing of indeterminate specimens for all specimens, culture-positive specimens, ... See full document
5
Comparison of a Commercial Real Time PCR Assay for tcdB Detection to a Cell Culture Cytotoxicity Assay and Toxigenic Culture for Direct Detection of Toxin Producing Clostridium difficile in Clinical Samples
... Cdiff assay and the TOX-B test are very similar to those of other investigators who have compared real-time PCR assays and cytotoxin or enzyme immunoassay (EIA) testing to anaerobic toxigenic culture (3, ... See full document
6
Toxin B PCR Amplification Cycle Threshold Adds Little to Clinical Variables for Predicting Outcomes in Clostridium difficile Infection: a Retrospective Cohort Study
... difficile PCR assay (Cepheid, Sunnyvale, CA, USA), a real-time PCR assay targeting the tcdB gene of ...difficile PCR assay was also performed on samples with a toxin A/B ... See full document
11
Severe clinical outcome is uncommon in Clostridium difficile infection in children: a retrospective cohort study
... PFGE data were available for 90 cultured C. difficile isolates from 129 nosocomial cases submitted to CNISP between August 1, 2008 and July 31, 2012 (Table 3). Thirty-four isolates (38%) did not have a fingerprint ... See full document
6
Risk factors for recurrent Clostridium difficile infection (CDI) hospitalization among hospitalized patients with an initial CDI episode: a retrospective cohort study
... a retrospective cohort study at Barnes-Jewish Hospital from 1/1/03 to ...toxin assay for C. difficile with no CDI in previous 60 days, and rCDI as a repeat positive toxin ≤ 42 days of ... See full document
8
Comparison of Real Time PCR for Detection of the tcdC Gene with Four Toxin Immunoassays and Culture in Diagnosis of Clostridium difficile Infection
... antigen”) assay was only 76% as sensitive as culture, calling into question the value of this assay as a surrogate marker for the pres- ence of ...(GDH) assay might have superior ...this study ... See full document
6
Comparison of Nine Commercially Available Clostridium difficile Toxin Detection Assays, a Real Time PCR Assay for C difficile tcdB, and a Glutamate Dehydrogenase Detection Assay to Cytotoxin Testing and Cytotoxigenic Culture Methods
... when using routine diagnostic samples, the great majority of which will yield negative results for ...C. difficile, despite the presence of ...C. difficile-positive samples is higher than routinely ... See full document
7
Evaluation of tcdB Real Time PCR in a Three Step Diagnostic Algorithm for Detection of Toxigenic Clostridium difficile
... In our study, CYT detected only 58.8% of positive speci- mens, comparable to previous studies describing the insensi- tivity of CYT (18, 22, 39, 46). The AB-Q, performed on GDH- Q-positive specimens, was more than ... See full document
7
Perirectal Swab Surveillance for Clostridium difficile by Use of Selective Broth Preamplification and Real Time PCR Detection of tcdB
... this assay is discrepant with toxigenic culture under the following circumstances: (i) detection of nonviable vegetative ...C. difficile with a poor sporulation capac- ity killed in ambient air before ... See full document
6
Real Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool
... A retrospective chart review was done to determine the severity of CDI among the study ...C. difficile or its toxins or colonoscopic or histopathologic findings demonstrating pseudomembranous colitis ... See full document
7
Risk prediction for 30-day mortality among patients with Clostridium difficile infections: a retrospective cohort study
... This retrospective cohort study was conducted at China Medical University Hospital, a 2111-bed tertiary medical center in central ...C. difficile culture or toxin assay and had diarrhea ... See full document
11
Comparison of BD GeneOhm Cdiff Real Time PCR Assay with a Two Step Algorithm and a Toxin A/B Enzyme Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection
... on study days and met study criteria, but only a subset of GDH antigen-negative samples, were in- ...C. difficile were ...in-house PCR assay to detect tcdC (the toxin repressor gene) ... See full document
6
A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile
... Six-week-old female BALB/c mice (6 mice per group) were obtained from the Laboratory Animal Unit (LAU) of The University of Hong Kong (HKU) and housed in the animal room of Department of Microbiology. All mouse ... See full document
8
Multiplex PCR Targeting tpi (Triose Phosphate Isomerase), tcdA (Toxin A), and tcdB (Toxin B) Genes for Toxigenic Culture of Clostridium difficile
... multiplex PCR (i) to distinguish C. difficile from other Clos- tridium species and (ii) to distinguish between the three main toxigenic types (A ⫹ B ⫹ , A ⫺ B ⫹ , and A ⫺ B ⫺ ) of ...C. difficile ... See full document
5
Antibodies for Treatment of Clostridium difficile Infection
... studies using vaccination/immunization and hence polyclonal sera and are summarized in Table ...produced using traditional (“high-cost”) CHO cell culture manufacturing ... See full document
11
Study of the frequency of Clostridium difficile tcdA, tcdB, cdtA and cdtB genes in feces of Calves in south west of Iran
... C. difficile was recognized as an intestinal pathogen in the late 70th of twentieth century ...C. difficile is agent for more than 95% of cases of pseudomembranous colitis and 15–25% of cases of ... See full document
6
False Negative Results in Clostridium difficile Testing
... This study investigated 48 patients that tested negative for toxigenic ...C. difficile via GeneXpert C. difficile epi test, while simultaneously testing positive for toxigenic ...C. difficile ... See full document
6
Mortality and Clostridium difficile infection: a review
... The search strategy used to identify articles for this re- view did not identify the same articles in the review pub- lished by Karas, Enoch & Aliyu (2010). Seven articles included in the review by Karas, Enoch and ... See full document
6
Rapid detection of toxigenic Clostridium difficile in fecal samples by magnetic immuno PCR assay
... Nucleotide sequence of Clostridium difficile toxin A gene fragment and detection of toxigenic strains by polymerase chain reaction.. Characterisation of Clostridium difficile strains by [r] ... See full document
5
Diagnosis of Clostridium difficile infection by toxigenic culture and PCR assay
... C. difficile in the intestine as well as the development of clinical symptoms associated with this infection (20, ...This study for the first time examined the fre- quency of toxigenic ...C. ... See full document
7
Related subjects