[PDF] Top 20 Induction of differentiation-associated changes in established human cells by infection with adeno-associated virus type 2.
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Induction of differentiation-associated changes in established human cells by infection with adeno-associated virus type 2.
... The mechanism of AAV-2-induced differentiation is not clear, and we do not know whether cessation of cell proliferation marked by a decrease of c-myc expression, arrest of cells in the G[r] ... See full document
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Subcellular compartmentalization of adeno-associated virus type 2 assembly.
... HeLa cells in early stages of infection or when expressed by transfection of the cap ...in cells in which the capsid proteins had entered the ...were cells in which nucleoli could no longer be ... See full document
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Encapsidation of adeno-associated virus type 2 Rep proteins in wild-type and recombinant progeny virions: Rep-mediated growth inhibition of primary human cells.
... AAV infection on primary and immortalized human cells in the absence of a helper virus and genotoxic stress (type I ...primary human cells at high particle-to-cell ... See full document
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Adeno-Associated Virus Type 2 Induces Apoptosis in Human Papillomavirus-Infected Cell Lines but Not in Normal Keratinocytes
... to adeno-associated virus type 2 (AAV2) less frequently than matched controls (27), suggesting that AAV2 has a “protective” effect against the development of this cancer ... See full document
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A novel terminal resolution-like site in the adeno-associated virus type 2 genome.
... adenovirus 2 (Ad2)-infected human cells and in some instances led to successful rescue and repli- cation of the AAV genome as well as the vector DNA se- quences (33, ... See full document
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Adeno-associated virus type 2-mediated transduction in primary human bone marrow-derived CD34+ hematopoietic progenitor cells: donor variation and correlation of transgene expression with cellular differentiation.
... 1 cells from negative donors, there was a significant increase in the b -Gal activity (up to 20-fold) in the myeloid fraction when CD34 1 cells were allowed to undergo cytokine-induced ... See full document
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Adeno-Associated Virus Type 2-Mediated Transduction of Human Monocyte-Derived Dendritic Cells: Implications for Ex Vivo Immunotherapy
... Adeno-associated virus (AAV) is a nonpathogenic parvovi- rus that infects a wide variety of cells both in vitro and in vivo (30, ...AAV infection (22), but more recent reports indicate ... See full document
9
Enhancement of Muscle Gene Delivery with Pseudotyped Adeno-Associated Virus Type 5 Correlates with Myoblast Differentiation
... C2C12 cells were due to differences in viral binding, as might be anticipated by altered capsid ...C2C12 cells (undifferentiated or differentiated) were incubated with rAAV-2 or rAAV-2cap5 ... See full document
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Induction of Robust Immune Responses against Human Immunodeficiency Virus Is Supported by the Inherent Tropism of Adeno-Associated Virus Type 5 for Dendritic Cells
... HEK293 cells were transduced with the AAV vectors encoding the HIV gene in a six-well ...the cells were washed in PBS and lysed with ...of 2⫻ sodium dodecyl sulfate buffer (125 mM Tris-HCl, pH ... See full document
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Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells.
... The adeno-associated virus type 2 (AAV)-based vector sys- tem appears to be promising in overcoming some of the limi- tations of retrovirus vectors (4, 8, ...replication-defective ... See full document
5
Major Subsets of Human Dendritic Cells Are Efficiently Transduced by Self-Complementary Adeno-Associated Virus Vectors 1 and 2
... the induction of differentiation and 12-day posttransduction CD34-LC and CD34-pDC trans- duced at day 5 or 6 with sc rAAV1 were potent activators of a CD8 ⫹ -T-cell clone specific for the tumor antigen ... See full document
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Detection of adeno associated virus type 2 sequences in the human genital tract
... Adeno-associated virus (AAV) is a defective parvovirus with unknown ...in human tissue and therefore is not readily isolated from clinical ...varicella-zoster virus infections, (iii) 21 ... See full document
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Infection of primary cells by adeno-associated virus type 2 results in a modulation of cell cycle-regulating proteins.
... fected cells at later time points ...AAV2-arrested cells, like contact-inhibited cells, to re- versibly remain in this state until a strong proliferative signal induces cell ...mary human ... See full document
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Adeno-Associated Virus Type 2 Rep Protein Inhibits Human Papillomavirus Type 16 E2 Recruitment of the Transcriptional Coactivator p300
... in cells. (B) Rep proteins inhibit HPV16 replication. 293 cells were transfected with an HPV16 ori-containing plasmid together with 16E1, 16E2, and the indicated Rep ...fluorescent cells was measured ... See full document
9
Consistent Viral Evolutionary Changes Associated with the Progression of Human Immunodeficiency Virus Type 1 Infection
... the changes in viral divergence (from an approximated founder strain; see Materials and Methods) were essentially the same in both cell-associated viral DNA ...participant 2, perhaps due to the ... See full document
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Cloning and Characterization of Adeno-Associated Virus Type 5
... simian virus 40 origin (SV40ori) fragment adjacent to the p5 promoter at the 59 end of the AAV5 RepCap fragment, and 5RepCapB has the SV40ori adjacent to the polyadenylation signal at the 39 ... See full document
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Adeno-Associated Virus Activates an Innate Immune Response in Normal Human Cells but Not in Osteosarcoma Cells
... inducing type I IFNs and the ensuing adaptive response to AAV ...U2OS cells with IFN-  significantly diminishes their permissiveness to ...U2OS cells at 24 and 48 h posttreatment. The cells ... See full document
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Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure
... The AAV capsid is composed of three related proteins, VP1, VP2, and VP3 (in order of decreasing size), present at a ratio of 1:1:10, respectively, and derived from a single gene by al- ternative splicing and alternative ... See full document
6
Adeno-Associated Virus Type 2 Protein Interactions: Formation of Pre-Encapsidation Complexes
... this type of complex has been discussed and linked to the nuclear uptake of parvo- virus genomes during an infection or to the release of assem- bled virus from the cell (8, 31, ... See full document
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Scalable Generation of High-Titer Recombinant Adeno-Associated Virus Type 5 in Insect Cells
... for type 2 rAAV production drove type 2 Rep72 expression with a truncated promoter for the immediate-early 1 gene of Orgyia pseudotsugata nuclear polyhedrosis virus ( ⌬ IE) and ... See full document
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