[PDF] Top 20 An inexpensive and rapid diagnostic method of Koi Herpesvirus (KHV) infection by loop mediated isothermal amplification

... A rapid field diagnosis of KHV in common and koi carp was developed using loop- mediated isothermal amplification ...under isothermal conditions using a simple ... See full document

... In comparison with the traditional technique based on virus isolation in embryonated chicken eggs, LAMP can directly detect NDV from samples and would be relatively inexpensive and efficient. Virus isolation in ... See full document

... diagnostic tests (RDT), using blood obtained from finger pricks, are now widely used, especially where quick and easy diagnosis of malaria is needed [2,3,5-9]. A major disad- vantage of RDTs is that they are ... See full document

... Research-oriented diagnostic definitions of inva- sive fungal infection (IFI) were created in 2002 and modified in 2008 by the European Organization for Research and Treatment of Cancer/My- coses Study ... See full document

... orimetric loop-mediated isothermal amplification (LAMP) assays targeting the ...loa infection compared to the absence of infection or infection with related filarial ... See full document

... A diagnostic test to be prospected as a point-of-care test and to be used in the field settings must qualify two basic criteria: a) high sensitivity and specificity & b) economical and easy to per- ...a ... See full document

... and rapid diagnostic method is ...natural infection during the course of the illness, 10 samples from patients with vaccine-associated parotitis, and 5 samples from patients with ...and ... See full document

... simple, rapid, and reliable methods for the diagnosis of serotype 1 MDV to prevent further disease transmission or ...the diagnostic method used most ...under isothermal conditions and does ... See full document

... HSV-2 infection is not crucial for correct administration of antiviral drugs, this discrimination is impor- tant from an epidemiological or public health ...detection method than agarose gel electrophore- ... See full document

... the amplification of viral DNA. Moreover, all amplification steps are completed in a conven- tional heat block and require only 30 min to ...detect amplification products, gel electrophoresis was ... See full document

... Human African trypanosomiasis is endemic in tropical Africa. In eastern and southern Africa the disease is caused by Trypanosoma brucei rhodesiense, while T. b. gambiense infections are common in central and West Africa. ... See full document

... HHV-7 infection usually has a benign and self-limited clinical course but can in rare instances cause several severe complications (16, ...reliable rapid diagnostic procedure for the detection of ... See full document

... acid amplification methods such as polymerase chain reaction (PCR) have been employed, enabling fast and sensitive detection of bacteria ...novel diagnostic methods. A powerful innovative nucleic acid ... See full document

... primary infection to disease ...traditional method for many decades ...ZN method, auramine O stained fluorescence microscopy that improves sensitiv- ity and takes less time to perform has been ... See full document

... the rapid detection and control of N. caninum infection in dogs are required and have been an objective for many studies ...small-scale diagnostic laboratories where established PCR-based methods are ... See full document

... a rapid and simple detection tool for the specific diagnosis of ...a diagnostic system [11]. The LAMP method used for GPV detection was observed to be highly sensitive, and other studies have shown ... See full document

... practical diagnostic method [18]; however, despite its excellent sensitivity and specificity, a PCR approach requires special equipment and is not suitable for field ...tion method based on ... See full document

... Loop-mediated isothermal amplification (LAMP), the novel method developed by Notomi et al [4], is able to amplify DNA with great efficacy as its detection limit for DNA in the reaction ... See full document

... The diagnostic performance of this new protocol was comparable to our earlier developed Mu RPA assay on archived DNA extracted with a purification kit (the sensitivity and specificity were 88% and 100%, ... See full document

... PCR targeting the trnL–trnF intergenic spacer region was conducted at the final volume of 25 µL with the fol- lowing components: 2.5 µL of 1× PCR buffer (Takara), 0.2 mM each dNTP, 0.5 µM each of forward primer (5ʹ- 3ʹ; ... See full document