[PDF] Top 20 Integrase mutants of human immunodeficiency virus type 1 with a specific defect in integration.
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Integrase mutants of human immunodeficiency virus type 1 with a specific defect in integration.
... A previous analysis of single amino acid substitutions in the human immunodeficiency virus type 1 HIV-1 integrase protein failed to identify mutations that only blocked the integration o[r] ... See full document
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Nuclear Localization of Human Immunodeficiency Virus Type 1 Preintegration Complexes (PICs): V165A and R166A Are Pleiotropic Integrase Mutants Primarily Defective for Integration, Not PIC Nuclear Import
... each virus was released from transfected cells at the WT level, we focused our attention on the early steps of HIV-1 ...HIV- 1 NL4-3 , class I mutant HIV-1 D64N/D116N (40), and class II ... See full document
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Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection.
... IN mutants impair intracellular viral DNA synthesis without affecting in vitro RT activity or RNA ...a defect not appreciated when virus stocks were assayed for RT activity in vitro with ... See full document
8
Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication.
... wild-type virus following infection of CEM-12D7 cells, and although the mutant virus was unable to spread throughout the culture and generate progeny virions as detected by RT assays ...(Table ... See full document
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Oligomerization within Virions and Subcellular Localization of Human Immunodeficiency Virus Type 1 Integrase
... for integration is com- posed of two dimers of IN ...of integration in vitro (20, ...during virus replication because of the lack of a sensitive detection ... See full document
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Monoclonal antibodies against human immunodeficiency virus type 1 integrase: epitope mapping and differential effects on integrase activities in vitro.
... group 1 (5F8 and 1C4) inhibited end processing and DNA joining less at low concentrations of MAb, but at higher concentrations ( $ 800 nM) these MAbs also gave close to 100% ...and integration, none of the ... See full document
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Structure-based mutagenesis of the catalytic domain of human immunodeficiency virus type 1 integrase.
... (ii) Lys-136. There are two dimers in the HIV-1 IN struc- ture, one with an extensive interface and the other with a much smaller contact area (13). Lys-136 interacts with Asp-116 across the minor dimer interface ... See full document
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Human Immunodeficiency Virus Type 1 Integrase Protein Promotes Reverse Transcription through Specific Interactions with the Nucleoprotein Reverse Transcription Complex
... the defect in the replica- tion of various IN mutant viruses could be complemented by the Vpr-IN fusion protein (20, ...the specific nature of the defect or the mechanism by which the trans-IN ... See full document
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Concerted integration of retrovirus-like DNA by human immunodeficiency virus type 1 integrase.
... full-site integration events, three independent scaled-up reac- tions were ...experiment 1, the virions were lysed at 50 mM NaCl and the strand transfer reaction was at 30 mM ...(Table 1). The ... See full document
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Analysis of Natural Sequence Variation and Covariation in Human Immunodeficiency Virus Type 1 Integrase
... HIV-1 integrase inhibitors (Table 1) showed that mutations V72I and V201I were ex- tremely prevalent across all subtypes and occur in 80 and 46% of these 1,165 sequences, respectively ...41 ... See full document
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Nuclear Import Defect of Human Immunodeficiency Virus Type 1 DNA Flap Mutants Is Not Dependent on the Viral Strain or Target Cell Type
... using human immunodeficiency virus type 1 (HIV-1) strain LAI, that the HIV-1 central DNA Flap acts as a cis determinant of viral genome nuclear ...infectious virus ... See full document
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Mutational Scan of the Human Immunodeficiency Virus Type 2 Integrase Protein
... Tables 1 and 2). Single mutants I110R, T111I, and C182S showed an increase in intermolecular disintegration activity, whereas in- tramolecular disintegration activity as well as cleavage and ... See full document
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Targeting Human Immunodeficiency Virus (HIV) Type 2 Integrase Protein into HIV Type 1
... the virus life cycle. Host cell proteins that bind to IN or the HIV-1 preintegration complex (PIC) and promote integration have been identified (13, ... See full document
6
Cofactors for Human Immunodeficiency Virus Type 1 cDNA Integration In Vitro
... (RT), integrase (IN), matrix (MA), Vpr, and nucleocapsid (NC), and recruits host factors, including HMGa1 [formerly HMG I(Y)] and Ku, to form the preinte- gration complex (PIC) (2, 11, 12, ...and ... See full document
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Pharmacovirological Impact of an Integrase Inhibitor on Human Immunodeficiency Virus Type 1 cDNA Species In Vivo
... a 1:250 dilution of a first-round aliquot was subjected to amplification with primers with the sequences 5⬘-ATGCCACGTAAGCGAAACTCTGGCTAACTA GGGAA CCCACTG-3⬘ and 5⬘-TGACTAAAAGGGTCTGAGGGATCT-3⬘ and the probe ...and ... See full document
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Integrase Interacts with Nucleoporin NUP153 To Mediate the Nuclear Import of Human Immunodeficiency Virus Type 1
... the 1-LTR circle form of viral DNA (top), total viral DNA (middle), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (bottom) from genomic ...Lane 1 shows nontransduced 293T ... See full document
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Genetic analysis of human immunodeficiency virus type 1 integrase and the U3 att site: unusual phenotype of mutants in the zinc finger-like domain.
... IN mutants of HIV-1, each with a single or double amino acid substitution in the catalytic D,D35E region or the HHCC zinc finger-like motif, as well as two different att site mutants designed to ... See full document
10
A Sensitive, Quantitative Assay for Human Immunodeficiency Virus Type 1 Integration
... HIV-1 specific, since they were absent from uninfected CEM-SS cells ...(Fig. 1), these numbers reflect the total number of proviruses in each ... See full document
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Conserved sequences in the carboxyl terminus of integrase that are essential for human immunodeficiency virus type 1 replication.
... of human immunodeficiency virus type 1 integrase (HIV-1 IN), W-235, the requirement for which is only revealed in viral assays for integrase function ...of ... See full document
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Coupled Integration of Human Immunodeficiency Virus Type 1 cDNA Ends by Purified Integrase In Vitro: Stimulation by the Viral Nucleocapsid Protein
... affect integration in vitro, raising the question of which are most likely to be important ...ASLV integrase three- to fivefold in vitro, providing another candidate ...late integration in these ... See full document
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