[PDF] Top 20 Integration is essential for efficient gene expression of human immunodeficiency virus type 1.

... Together with an observation that the mutant lacked the ability to integrate, these results indicated that the integration was required for efficient viral gene expression and productive[r] ... See full document

... (13). Integration of HIV is usually required for efficient viral replication and expression, although recent reports sug- gest that transcription from nonintegrated HIV DNA appears to be a normal ... See full document

... Chronic HIV-1 infection of U9-IIIB cells resulted in a low constitutive level of transcription of TNF and IL-1 genes but not IFN genes; however, when the cells were coinfected with Senda[r] ... See full document

... reporter gene expression from proviruses integrated in the murine genome increased threefold upon fusion to 293T ...reporter virus-infected ...provirus expression. Thus, the increase in ... See full document

... cells, human immunodeficiency virus type 1 (HIV-1)-infected macrophages typically resist cell death, support viral replication, and consequently, may facilitate HIV-1 ... See full document

... ence of antisense RNA, we cotransfected 293T cells with the pNL4-3 provirus lacking a functional rev gene, pCMV-Rev, and the RRE-driven sense or antisense plasmids. The provirus and sense or antisense plasmids ... See full document

... full-site integration products produced by these preparations ...half-site integration because ⬃25% of the input donor is in- corporated in 20-min ...full-site integration. This minority population ... See full document

... from 1 1 to 1 60 in the human immunodeficiency virus type 1 (HIV-1) long terminal repeat, which is critical for activation by the transactivator protein ... See full document

... In addition, in all three cell types used in this study peripheral blood lymphocytes, Mo-T, and MT-4, infection with wild-type HIV-1 mfA virions and integrase mutants resulted in the syn[r] ... See full document

... for 1 h accu- mulated ⬃ 90 initiated transcripts and ⬃ 4 elongated transcripts per ...for 1 h, whereas elongated transcript formation was reduced to levels below the limit of detection ...preceded ... See full document

... more efficient at stimulat- ing integration than the NC ...most efficient stimulatory ...stimulate integration under slightly different reaction conditions (7), though several other reports ... See full document

... Previous microarray studies on HIV-1 have used infection of cell lines at low multiplicity of infection (MOI) (MOI, 0.1 to 0.5) to study changes up to 3 days after infection (8, 15, 39) or heterogeneous target ... See full document

... bacterial expression systems or purified from avian myeloblas- tosis virus (AMV) has been coupled with the use of oligonu- cleotides containing viral DNA terminal sequences to investi- gate both the 3 9 OH ... See full document

... for expression in HEK293T cells by transfection fol- lowed by immunoblot analysis as shown for VN-Vpr constructs in ...The expression level of all Vpr mutant molecules is compara- ble to wild type, ... See full document

... Sindbis expression system, which rep- licates in the ...protein expression, we cloned both wild- type gag and INS mutant gag genes into a Sindbis expression ...Sindbis expression system ... See full document

... infected WE17/10 cell line, were monitored for 50 to 60 weeks p.i. The murine monoclonal antibodies used included OKT.3 (anti-CD3; Ortho Diagnostic Systems, Beerse, Bel- gium), OKT.4 (anti-CD4 that recognizes a ... See full document

... in human glial cells in vitro as a model for direct interaction of HIV-1 with nervous system-derived ...viral expression in these cells is about 3 orders of magnitude less efficient than that ... See full document

... (Fig. 1). An enhancement of C2 mRNA expression by HIV-1 was seen already after 1 day of incubation; the maximal difference was a 6-to-10-times higher level of infected astrocytes than of ... See full document

... To determine the contribution of cell division to the HIV-1 replicative cycle in T cells, we evaluated HIV-1 expression, integration of proviral DNA, and production of infectious progeny[r] ... See full document

... cycle-dependent gene expression, pCEP and eTat cells were reversibly blocked with Hu for 18 h and then ...the expression of a total of seven different cytokine and chemokine genes, including the ... See full document