[PDF] Top 20 Interactions between Hepatitis Delta Virus Proteins

... arated at aa 49 by proline (29). To form a dimer, the long helices of each of two monomers wrap around each other, forming an antiparallel coiled-coil (29). In addition, each dimer associates with three other dimers, ... See full document

... This report focuses on the use of immunostaining proce- dures to study the associations of ␦ Ag in the presence and absence of HDV RNAs. The advantage of this approach is that if the cells are fixed prior to staining, ... See full document

... binding domains (Fig. 1). These proteins had at least one of the three RNA-binding sequences mutated. For examples, SmA1DC contained Arg-1043Gln and Arg-1053Gly muta- tions in the ARM I. SmA29DC contained ... See full document

... 53]) between ORF52 and ORF62, in which the former had K and the latter had ...envelope proteins using six different PCR assays, which employed the pairs of primers FA, BA, E1A, E2A, CA, and ... See full document

... fusion proteins directly disturbed the ability of the viral proteins to interact with ApoE (unpublished data), and no interaction domain on the envelope proteins could be ...exists between the ... See full document

... RNA-protein interactions represented by PKR- RNA binding uses one or more copies of a motif found in proteins that bind dsRNA (17, ...both proteins to extensive perfect RNA-RNA du- plexes compared ... See full document

... Another issue concerns how d Ag-L was phosphorylated. A clue to this came when we mutated the cysteine at aa 211 to alanine. Others have shown that this is the site at which d Ag-L can be isoprenylated (10), a ... See full document

... It is not clear why LHDAg is much more efficient than SHDAg in this ribozyme enhancement function. LHDAg and SHDAg have very similar RNA-binding activities (15). It is possible that LHDAg recruits certain cellular ... See full document

... isolate-specific interactions between HDAg and HDV ...interaction between HDAg-S of geno- types I and III such that the replication activity of genotype I HDAg is ... See full document

... the interactions between the promoter sequence (IG) and cellular proteins correlated with the amounts of subgenomic mRNA tran- ...the interactions among the regulatory RNA sequences for ... See full document

... of virus disruption by nonionic detergent and DTT and the ability to recover the released RNP free of associated or contaminating complexes of surface ...proteins. Virus-like particles secreted by ... See full document

... FIG. 1. Effect of different electrophoresis time periods (NEPHGE) in sepa- rating isoforms of S- and L-HDAgs in a two-dimensional system. L-HDAg- and S-HDAg-expressing constructs (pCDAg-L and pCDAg-S) were cotransfected ... See full document

... fusion proteins in bacteria. Each of the mutated proteins consisted of substitutions of two arginines in the cores of either ARM I (RRRK) or ARM II ...RNA-protein interactions in the absence of ... See full document

... FIG. 1. Immunofluorescence of cells transfected with HDV cDNAs and examined by standard (A through P) or confocal microscopy (Q through T). (A through L) Four fields of cells stained three ways: for dAg (A to D), SC35 (E ... See full document

... Are the events which lead to genome replication specific for d Ag-S? It needs to be pointed out that the assay of transfection with RNP as described above, involves not one but two “sources” of functional dAg-S. The ... See full document

... affinity between S and HDV RNPs as previously suggested ...Protein-protein interactions between S and the p27 HDV protein are thought to provide the molecular basis for virion assembly, which is ... See full document

... C virus (HCV), the sole member of the Hepacivi- rus genus, possesses a single-stranded, sense RNA genome approximately 9,600 nucleotides in length ...(NS) proteins and yet-unidentified cellular ... See full document

... Next, we examined the effect of different MOIs using qPCR, and the results are summarized in Fig. 4. In a range of MOIs up to 1,000 GE/cell, wHDV readily infected PWH, while the same cells were resistant to hHDV. ... See full document

... cannot exceed the above-described 6.6%. Therefore, the fraction of the PreS1-containing virions among the HDV-D1 virions pre- cipitated with the antimatrix antibodies is expected to be greater or equal to 83.2% [100% ⫺ ... See full document

... C virus (HCV) is recognized as the main etiologic agent of parentally transmitted non-A, non-B hepatitis (8, 23) and is responsible for a large portion of community-acquired hepatitis ...chronic ... See full document