[PDF] Top 20 Kinetochore dynamics and their attachment to the mitotic spindle

... therefore kinetochore position (Church- man et ...the spindle is undergoing rotations and translations within the cell it is important to ensure that a rotation-free coordinates system is used to accurately ... See full document

... the mitotic spindle undergoes functional maturation until the satisfaction of the spindle assembly checkpoint (SAC), which prevents anaphase until all chromosomes have formed bipolar ... See full document

... bipolar mitotic spindle is made from dif- ferent subclasses of MTs depending on their position, functionality and organization: astral MTs, interpolar MTs and K-fibers ...and spindle positioning ... See full document

... stained spindle MTs in HeLa cells, particularly in the vicinity of spindle poles in early mitosis before concentrating at the midbody in late mitosis ...to spindle MTs in HEK 293 cells, indicating ... See full document

... the mitotic spindle microtu- bules that emanate from abnormally positioned centrosomes can- not efficiently probe the kinetochore landscape ...in spindle geometry early in mitosis, though most ... See full document

... describes the in vitro and in vivo behaviour of MTs wherein MT plus ends undergo dynamic instability, with stochastic switching from growth to shortening (‘catastrophe’), and from shortening to growth (‘rescue’). Thus ... See full document

... chromosome attachment and bi-orientation in mitosis. In diploid cells, each spindle pole body nucleates at least 36 microtubules (32 kinetochore microtubules plus 4 interpolar ...The spindle ... See full document

... inner kinetochore, associated with centromeric DNA, and more dynamic outer kinetochore, which is formed around the time of the nuclear envelope breakdown (reviewed in Maiato et ...the kinetochore is ... See full document

... or antibody injection showed that Spc24 is required not only for establishing, but also for maintaining K-MT interactions in mitotic cells [19]. In addition, Spc24 is required for chromosomal metaphase alignment ... See full document

... The spindle assembly checkpoint chromosomal structures (reviewed by Skibbens and serves to arrest cells at prometaphase until each sister Hieter ...The kinetochore localization and associ- chromatid pair ... See full document

... (the spindle pole bodies in yeast), microtubule sis by activating a multiprotein complex [the cyclosome motors (reviewed in Hoyt and Geiser 1996; Stearns or anaphase-promoting complex (APC)] that catalyzes 1997), ... See full document

... the spindle apparatus attaches correctly to the kinetochores of chromosomes from the appropriate ...priate attachment of microtubules to chromosomes of the in- correct nucleus is to enclose each ... See full document

... in mitotic events, including activation of CDC25c phosphatase, regulation of microtubule nucle- ation, centrosome maturation and kinetochore assembly ...at mitotic entry by Aurora A kinase and its ... See full document

... and mitotic exit. Thanks to this spatial segregation, improper spindle and nuclear posi- tioning would prevent Tem1 activation and delay mitotic exit and cytokinesis until errors are corrected, thus ... See full document

... (with centriole pair) Sister chromatids Chiasmata Spindle Homologous chromosomes Fragments of nuclear envelope Centromere (with kinetochore) Metaphase plate Microtubule attached to kinet[r] ... See full document

... Results: Treatment of HeLa cells with varying concentrations of SP600125 induces significant G2/M cell cycle arrest with elevated phosphorylation of histone H3 within 48 h, and endoreduplication after 48 h. SP600125 also ... See full document

... bipolar spindle assembly underlies accurate chromosome ...orchestrates spindle microtubule formation in a spatiotemporally coordinated ...early mitotic arrest with very short, fragile ...undergo ... See full document

... a kinetochore-based pushing force (F1) combines with cortical forces (mediated by actin and myosin) and extensile MT sliding (mediated by Eg5 [F2]) to generate a force with suffi cient magnitude and correct ... See full document

... Kin4 kinase activity is absolutely vital for SPOC func- tion. Lately, we and others have shown that Kin4 kinase activity requires another kinase, namely Elm1 [106,110]. Elm1 is a bud neck localized kinase which is ... See full document

... The idea of multiple microtubule-binding sites on by the spindle in opposite directions (Figure 3, C and each sister kinetochore is supported by observations (we D). In common wheat, the[r] ... See full document