[PDF] Top 20 Fast Ligation Mediated PCR, a Fast and Reliable Method for IS6110 Based Typing of Mycobacterium tuberculosis Complex
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Fast Ligation Mediated PCR, a Fast and Reliable Method for IS6110 Based Typing of Mycobacterium tuberculosis Complex
... different IS6110 RFLP patterns and its high reproducibility is only reached by the newly developed variable-number tandem repeat (VNTR) typing methods (11, 11a, 14, ...VNTR typing differs according ... See full document
6
PCR Based Method To Differentiate the Subspecies of the Mycobacterium tuberculosis Complex on the Basis of Genomic Deletions
... tests based upon growth, phenotypic, and biochemical properties have been traditionally used to segre- gate members of the MtbC (17, ...of PCR products followed by restriction frag- ment length polymorphism ... See full document
14
Variable Number Tandem Repeat Typing of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110 by Using Mycobacterial Interspersed Repetitive Units
... of PCR-based typing methods—rapid turnaround, no requirement for large quantities of DNA, and the ability to use nonviable samples—and in addition provides a simple dig- ital ...during PCR can ... See full document
11
McSpoligotyping, a One Step Melting Curve Analysis Based Protocol for Spoligotyping of Mycobacterium tuberculosis
... a ligation-based spoligotyping method that can identify unamplified spacers in membrane-based spoligotyping because of asymmetric inser- tion of IS6110 in the direct repeat locus ...This ... See full document
8
Stability of Variable Number Tandem Repeats of Mycobacterial Interspersed Repetitive Units from 12 Loci in Serial Isolates of Mycobacterium tuberculosis
... VNTR typing systems for M. tuberculosis complex strains made use of very limited sets of loci (6, 8, 14, 15, 17), which turned out not to be sufficiently discriminatory ...system based on 12 ... See full document
6
Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains
... RFLP typing method is, however, laborious and requires the preparation of several micrograms of chro- mosomal DNA, a procedure which may take several weeks because of the slow growth rate of ...alternative ... See full document
5
Discriminatory Power and Reproducibility of Novel DNA Typing Methods for Mycobacterium tuberculosis Complex Strains
... DNA typing methods have been developed which are faster and easier to perform than the current internationally standardized IS6110 restriction fragment length polymorphism typing ...novel ... See full document
11
Utility of New 24 Locus Variable Number Tandem Repeat Typing for Discriminating Mycobacterium tuberculosis Clinical Isolates Collected in Bulgaria
... DNA typing methods have been developed that are faster and easier to perform than the IS6110-RFLP ...(VNTR) typing is probably the most popular ap- proach. This method is based on the ... See full document
7
Rapid Detection of Mycobacterium tuberculosis in Respiratory Samples by Transcription Reverse Transcription Concerted Reaction with an Automated System
... of Mycobacterium tuberculosis complex (MTC) in respiratory samples are extremely important for optimal diagnosis and effective treatment, as well as for prevention and control of tuberculosis ... See full document
5
Use of a PCR method based on IS6110 polymorphism for typing Mycobacterium tuberculosis strains from BACTEC cultures
... of tuberculosis can be greatly facil- itated by the application of strain-specific ...type Mycobacterium tuberculosis complex strains, most molec- ular methods are based on the ... See full document
5
Polymorphism of Variable Number Tandem Repeats at Multiple Loci in Mycobacterium tuberculosis
... fingerprinting method based on restric- tion fragment length polymorphism (RFLP) of IS6110 inser- tion sequences has been established as the standard for typing strains of Mycobacterium ... See full document
10
Evaluation of Mycobacterium tuberculosis Typing Methods in a 4 Year Study in Schleswig Holstein, Northern Germany
... to IS6110 RFLP typing for discrimination of clinical isolates and detection of transmission chains in TB patient populations ...VNTR typing with the “gold standard” IS6110 RFLP typing, ... See full document
6
Molecular Fingerprinting of Mycobacterium bovis subsp caprae Isolates from Central Europe
... To study the dissemination of Mycobacterium bovis subsp. caprae, 79 European isolates from cattle, humans, and other hosts were examined by spoligotyping and IS6110 restriction fragment length polymorphism ... See full document
5
Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl Neelsen stained slides extracted DNA
... Since we wanted to provide a detailed spatial analysis of circulating MTC genotypes, we stud- ied M. tuberculosis diversity in 36 states using ZN-stained smears representative of the country to build geographical ... See full document
13
Molecular typing of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients in central Ethiopia
... Effective TB control program requires understanding of its epidemiology including the strains of M.tuberculo- siscomplex (MTBC) circulating in the population [3]. Molecular epidemiological studies have been used to ... See full document
8
Direct identification and typing of Mycobacterium tuberculosis by PCR
... automated method for analyzing the complex band patterns generated by the PCR fingerprinting ...a fast, quantitative method for automated detection of DNA bands in this fingerprinting ... See full document
6
A Combination of Two Genetic Markers Is Sufficient for Restriction Fragment Length Polymorphism Typing of Mycobacterium tuberculosis Complex in Areas with a High Incidence of Tuberculosis
... M. tuberculosis complex strains in Madagascar, a country in which TB is hyperendemic ...two-genetic-marker typing method (IS6110 and DR), we found three IS6110 clusters ... See full document
6
Identification of a New DNA Region Specific for Members of Mycobacterium tuberculosis Complex
... M. tuberculosis complex (47) and is, in addition, used for the typing of ...M. tuberculosis strains by RFLP analysis (29, 46). However, IS6110 is a member of the IS3 family, the most ... See full document
7
A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples
... Our samples were four sputum samples 1 ml per portion, one liver biopsy sample 10 mg per portion, and one feces sample 600 ,ul per portion from patients suffering from diseases other tha[r] ... See full document
7
Molecular Characterization of Mycobacterium tuberculosis Isolates from Different Regions of Bulgaria
... M. tuberculosis subspecies such as ...M. tuberculosis sensu ...the IS6110- low banding X clade (3 sublineages), and an ill-defined T clade (5 ... See full document
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