[PDF] Top 20 Live Visualization of Herpes Simplex Virus Type 1 Compartment Dynamics

... recombinant virus rep- licated at a rate comparable to that of wild-type (wt) virus ...recombinant virus was replication competent and showed stable autofluorescence ... See full document

... The ocular route of infection was predominately used in the present investigation to assess the safety of the HSV-1 0ΔNLS vaccine from the perspective of viral pathogenesis and tissue immunopathology although this ... See full document

... in live cells at the first time point therefore reflects only the very rapidly diffusing histone molecules, which are considered unbound to chromatin ...than 1 standard deviation above the average level of ... See full document

... helper virus. According to our results, however, the situation with HSV-1 as the helper virus clearly appears to be different from that described for Ad-aided AAV replication, in which AAV DNA ... See full document

... the type pIEn-AFP-ICPm, where n is IE1 or IE3, AFP is either ECFP or EYFP, and m is either 0 or ...isolate virus vEYFP-ICP0, the plasmid DNA was cotrans- fected into BHK cells with the ICP0 null mutant ... See full document

... Herpes simplex virus type 1 (HSV-1) and HSV-2 are closely related alphaherpesviruses that cause lifelong infections for which there is no ...genital herpes is changing in ... See full document

... prominent compartment of the cell nucleus and the most extensively studied nuclear ...replication, virus assembly, and control of intracellular trafficking (for reviews see references 22 and ... See full document

... Visualization of tegument-capsid interactions. Figure 3a shows a surface view of the virion reconstruction contoured at 1s. At this density threshold, which is higher than that used for Fig. 1, the ... See full document

... of herpes simplex virus type 1 (HSV-1), recent evi- dence suggests that the association of viral genomes with ND10 may be functionally important, because genomes that are so ... See full document

... Moreover, weak neutralizing antibodies were found in the sera of mice after oral inoculation of UV-inactivated or heat-inactivated HSV-1 data not shown, but these mice were not protected[r] ... See full document

... ficiency virus (HIV), it has been demonstrated that these en- veloped RNA viruses can recruit endosomal sorting complexes required for transport (ESCRTs) via interaction with late- domain (L-domain) motifs ... See full document

... The assay was performed in the presence of M13-22-mer substrate, 0.27 ,uM ICP8, and the indicated concentrations of MgCI2 as described in Materials and Methods.. Kinetics of strand displ[r] ... See full document

... We used hybrid-arrested translation to demonstrate that the 155,000-dalton polypeptide encoded by the 6-kb mRNA mapping in HindIII fragment J 0.181 to 0.259 is the only species of that s[r] ... See full document

... The effect of tunicamycin on the synthesis of herpes simplex virus type 1 HSV-1 KOS glycoproteins was examined by analysis of the proteins synthesized in infected MRC-5 cell cultures mai[r] ... See full document

... One of these, group VII antibody, was shown to react with a type-common continuous epitope within residues 11 to 19 of the mature glycoprotein residues 36 to 44 of the predicted sequence[r] ... See full document

... We report herein the genetic and phenotypic characterization of four temperature-sensitive mutants of herpes simplex virus type 1 tsY46, tsE5, tsE6, and tsLG4 that have begun to elucidat[r] ... See full document

... If the coprecipitation of the pKAlB polypeptide in the presence of pK230 is due to interactions between two upstream sites, then the upstream site is localized to amino acid residues 235[r] ... See full document

... It is also possible that early gene expression in cells infected by HSV-1 strains which have an intact IE 3 gene contributes to the induction of CPE, particularly since IE 3-expressing s[r] ... See full document

... Effects of HSV ICP27 on ICP4 phosphorylation and func- tion. Studies with infected cells (28, 41) and transfected cells (53) have shown that ICP27 leads to an electrophoretic shift in ICP4 and stimulation of late gene ... See full document

... The network we have described is reminiscent of the net- work formed during late phage T4 replication. In the case of phage T4, genomic ends invade homologous sequences on oth- er genomes to produce a Y junction that ... See full document