[PDF] Top 20 Localization and putative function of the UL20 membrane protein in cells infected with herpes simplex virus 1.
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Localization and putative function of the UL20 membrane protein in cells infected with herpes simplex virus 1.
... With the aid of this antiserum and an antibody to a constituent of the Golgi aparatus, we determined the following: i the UL20 gene is regulated as a -Yl gene, and the protein can be det[r] ... See full document
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Identification, Localization, and Regulation of Expression of the UL24 Protein of Herpes Simplex Virus Type 1
... the function of UL24 during ...different membrane fusion ...and UL20, which encodes a virion-associated protein involved in viral transport from the nucleus to the cell membrane ... See full document
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Plasma Membrane Topology of Syncytial Domains of Herpes Simplex Virus Type 1 Glycoprotein K (gK): the UL20 Protein Enables Cell Surface Localization of gK but Not gK-Mediated Cell-to-Cell Fusion
... were infected with the indicated virus at an MOI of ...hpi, cells were washed with TBS and fixed with electron microscopy-grade 3% parafor- maldehyde (Electron Microscopy Sciences, Fort Washington, ... See full document
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Functional and Physical Interactions of the Herpes Simplex Virus Type 1 UL20 Membrane Protein with Glycoprotein K
... were infected with the indicated virus at an MOI of ...fixation cells were washed with TBS-Ca/Mg and incubated for 15 min at room temperature in EZ-Link sulfo-NHS-LC biotin cell-impermeative ... See full document
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Characterization of VP22 in Herpes Simplex Virus-Infected Cells
... For herpes simplex virus (HSV) the tegument accounts for approximately 50% of the volume of the virion and more than 15 virus-encoded proteins have been reported to be recruited to it ... See full document
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The Herpes Simplex Virus Type 1 UL20 Protein and the Amino Terminus of Glycoprotein K (gK) Physically Interact with gB
... Vero cells in six-well plates were transfected with 5 g of plasmid gKa or control plasmid containing the gB gene sequence cloned in a reverse orientation using Lipo- fectamine 2000 reagent (Invitrogen) according ... See full document
11
Membrane Association of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
... A7 cells with the plasmid encoding VP22- GFP followed by Western blot analysis ...chimeric protein was expressed and migrated at the expected molecular mass of approximately 64,000 ...intracellular ... See full document
11
A post-alpha gene function turns off the capacity of a host protein to bind DNA in cells infected with herpes simplex virus 1.
... Experiments involving i temperature shift-down of cells infected with tsLB2 in the presence of cycloheximide, ii withdrawal of cycloheximide in the presence and absence of actinomycin D [r] ... See full document
6
Herpes Simplex Virus 1 UL34 Protein Regulates the Global Architecture of the Endoplasmic Reticulum in Infected Cells
... the localization of CD98hc in HSV-1-infected cells, we constructed the UL34-null mutant virus YK722(ΔUL34) and its repaired virus, YK723(ΔUL34-repair) ...(Fig. 1). As ... See full document
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US3 of Herpes Simplex Virus Type 1 Encodes a Promiscuous Protein Kinase That Phosphorylates and Alters Localization of Lamin A/C in Infected Cells
... the cells in which a planar view of the nuclear lamina was most readily apparent ...HSV- 1(F)-infected cells, the reticular filaments were bordered by spaces that lacked eGFP ...fected ... See full document
12
Herpes simplex virus capsids assembled in insect cells infected with recombinant baculoviruses: structural authenticity and localization of VP26.
... mammalian cells and insect Sf9 ...capsid protein required for DNA packaging ...This protein would be expected to occupy a unique vertex, distinct from the other ... See full document
5
Overexpression of the herpes simplex virus type 1 immediate-early regulatory protein, ICP27, is responsible for the aberrant localization of ICP0 and mutant forms of ICP4 in ICP4 mutant virus-infected cells.
... these cells by immunoblot analysis produced vir- tually the same results ...3-3 cells (Fig. 6, lanes 1 and 8), and the levels of ICP27 expression increased as the multiplicities of infection of d92 ... See full document
11
Herpes Simplex Virus 1 Suppresses the Function of Lung Dendritic Cells via Caveolin-1
... structural protein of caveolae, has been implicated as a regulator of virus-host ...in herpes simplex virus 1 (HSV-1) infec- tion have not fully been ...dendritic ... See full document
13
Posttranslational modification and subcellular localization of the p12 capsid protein of herpes simplex virus type 1.
... In contrast, the TrpE-UL35 antiserum reacted with two proteins, designated pl2a and pl2b, which were present in lysates of HSV-infected cells radiolabeled with either [35S]methionine or [r] ... See full document
9
The herpes simplex virus UL20 protein compensates for the differential disruption of exocytosis of virions and viral membrane glycoproteins associated with fragmentation of the Golgi apparatus.
... We report the remarkable observation that in Vero cells infected with the UL20- virus, the viral glycoproteins associated with virions sequestered in the space between the inner and oute[r] ... See full document
9
UL20 Protein Functions Precede and Are Required for the UL11 Functions of Herpes Simplex Virus Type 1 Cytoplasmic Virion Envelopment
... UL11-null virus, R7219, carrying a UL11 deletion of 176 bp was constructed using classical recombination experiments ...UL11 virus carried an additional deletion of 18 ...R7219 virus, since both ... See full document
12
Association of a major transcriptional regulatory protein, ICP4, of herpes simplex virus type 1 with the plasma membrane of virus-infected cells.
... When identical samples were immunoblotted with anti-ICP4 serum, significant amounts of ICP4 were present in the purified plasma membrane fraction, suggesting that ICP4 was associated wit[r] ... See full document
9
Phenylalanine Residues at the Carboxyl Terminus of the Herpes Simplex Virus 1 UL20 Membrane Protein Regulate Cytoplasmic Virion Envelopment and Infectious Virus Production
... The herpes simplex virus type 1 (HSV-1) UL20 gene encodes a 222-amino-acid nonglycosylated envelope protein which forms a complex with viral glycoprotein K (gK) that ... See full document
10
Herpes Simplex Virus 1 Protein Kinase Us3 and Major Tegument Protein UL47 Reciprocally Regulate Their Subcellular Localization in Infected Cells
... a protein kinase is tightly regu- lated; ...other protein kinases, or interaction with other proteins ...activated protein kinase phosphorylates a substrate(s), and as a result, the phosphorylated ... See full document
15
Herpes Simplex Virus Type 1 Glycoprotein K and the UL20 Protein Are Interdependent for Intracellular Trafficking and trans-Golgi Network Localization
... retained protein caused drastic modifications in the intracellu- lar transport and localization of both gK and UL20p (compare ...panels 1, and 8A and B with ...panels 1 to ...cent cells ... See full document
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