[PDF] Top 20 Mapping of Sequences in Pseudorabies Virus pUL34 That Are Required for Formation and Function of the Nuclear Egress Complex
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Mapping of Sequences in Pseudorabies Virus pUL34 That Are Required for Formation and Function of the Nuclear Egress Complex
... of pUL34 shows little sensitivity toward alterations, the well-conserved N terminus is highly ...impairing pUL34 function and interac- tion with ...of pUL34 function correlated with ... See full document
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Identification of Conserved Amino Acids in pUL34 Which Are Critical for Function of the Pseudorabies Virus Nuclear Egress Complex
... ingly, pUL34 –N103A was unable to complement the defect of PrV- ⌬UL34, despite speckle formation indicative of pUL31 inter- action after ...no pUL34/pUL31-positive speckles were found after infection ... See full document
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Structural Determinants for Nuclear Envelope Localization and Function of Pseudorabies Virus pUL34
... herpesviral pUL34 homologs is not understood, nor are the molecular details of primary envelopment and the mechanism of fusion of the pri- mary virion envelope with the outer nuclear ...during ... See full document
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A Physical Link between the Pseudorabies Virus Capsid and the Nuclear Egress Complex
... capsid egress selectiv- ity: selection does not occur by restricting NEC binding to packaged ...are required for capsid nuclear egress and both are capsid-associated proteins (7, 35, ...the ... See full document
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Function of the Nonconserved N-Terminal Domain of Pseudorabies Virus pUL31 in Nuclear Egress
... ABSTRACT Nuclear egress of herpesvirus capsids is mediated by the conserved nu- clear egress complex (NEC), composed of the membrane-anchored pUL34 and its nucleoplasmic interaction ... See full document
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Glycoproteins Required for Entry Are Not Necessary for Egress of Pseudorabies Virus
... not required for the egress of PrV from either the nucleus or the ...the nuclear membrane and their dispensability for nuclear egress contrast findings from other ...for nuclear ... See full document
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Lysine 242 within Helix 10 of the Pseudorabies Virus Nuclear Egress Complex pUL31 Component Is Critical for Primary Envelopment of Nucleocapsids
... after formation of the ...The formation of primary envelopes in the presence of numerous mature capsids but with a defect in in the interaction interface as shown here for pUL31-K242A points to the ... See full document
13
Complex Formation between the UL16 and UL21 Tegument Proteins of Pseudorabies Virus
... pesvirus pseudorabies virus, monospecific antisera against bacterially expressed fusion proteins were gener- ...the formation of a physical complex. To investigate protein function, ... See full document
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The NS2 Proteins of Parvovirus Minute Virus of Mice Are Required for Efficient Nuclear Egress of Progeny Virions in Mouse Cells
... minute virus of mice (MVMp) were previously shown to interact with the nuclear export receptor ...their nuclear export signal (NES) ...four virus-encoded ...no virus could be recovered ... See full document
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Functional Characterization of Nuclear Trafficking Signals in Pseudorabies Virus pUL31
... functional nuclear import signal but also a ...NEC formation and impairs viral replication while inactivation of the corresponding motifs still allows partial NEC ...are required for efficient ... See full document
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A cellular function is required for pseudorabies virus envelope glycoprotein processing and virus egress.
... infection, there is a second block to export from late Golgi compartments (possible the trans-Golgi network) to the cell surface (indicated by the solid X). In addition, late after infec[r] ... See full document
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The Capsid-Associated UL25 Protein of the Alphaherpesvirus Pseudorabies Virus Is Nonessential for Cleavage and Encapsidation of Genomic DNA but Is Required for Nuclear Egress of Capsids
... terminase-genome complex generates the packaging motor, which pumps the DNA against growing mechanical forces into the capsid, fueled by ATP hydrolysis, resulting in removal of the internal scaffold ...inner ... See full document
12
The UL20 gene product of pseudorabies virus functions in virus egress.
... differences, virus particles local- ized in distinct compartments of either Vero or VneoB4 cells infected with PrV DUL20Z were counted in randomly selected electron micrographs of 10 cells each and the subcellular ... See full document
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The UL7 Gene of Pseudorabies Virus Encodes a Nonessential Structural Protein Which Is Involved in Virion Formation and Egress
... simplex virus type 1 are conserved in alpha-, beta-, and gammaher- ...alphaherpesvirus pseudorabies virus ...maximum virus titers were decreased nearly 10-fold and plaque diameters were ... See full document
9
Identification and characterization of the pseudorabies virus UL3.5 protein, which is involved in virus egress.
... and virus mutants were isolated after the rescue of defective, glycoprotein B-negative PrV by insertion of the complementing glycoprotein B-encoding gene of bovine herpesvirus 1 at two sites within the ...plaque ... See full document
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Nucleolin Is Required for Efficient Nuclear Egress of Herpes Simplex Virus Type 1 Nucleocapsids
... the nuclear membrane for final assembly and maturation to form infectious progeny virions in the ...inner nuclear membrane, and these enveloped nucleo- capsids then fuse with the outer nuclear ... See full document
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Nuclear Egress of Pseudorabies Virus Capsids Is Enhanced by a Subspecies of the Large Tegument Protein That Is Lost upon Cytoplasmic Maturation
... detect nuclear reactivity by immu- nofluorescence, and by immunoelectron microscopy no VP1/2 was detected on capsids resident in the lumen of the nuclear en- velope ...do nuclear C capsids, one ... See full document
12
Autographa californica Multiple Nucleopolyhedrovirus ac75 Is Required for the Nuclear Egress of Nucleocapsids and Intranuclear Microvesicle Formation
... is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm (33), and the BM61 protein has been identified as a structural component of both BVs and ODVs ...knockout ... See full document
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Functions of the sequences at the ends of the inverted repeats of pseudorabies virus.
... internal repeat has a 143-bp deletion at its terminus, but the internal BamHI fragment that is generated will include 24 bp of M13 DNA.) The result of a Southern blot analysis of a BamHI[r] ... See full document
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Characterization and mapping of a nonessential pseudorabies virus glycoprotein.
... Antigenic variants of pseudorabies virus (PRV) containing mutations in a viral glycoprotein with a molecular weight of 82,000 (gIll) were isolated by selecting for resistance to a comple[r] ... See full document
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