[PDF] Top 20 Membrane Interface-Interacting Sequences within the Ectodomain of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein: Putative Role during Viral Fusion

... Viral fusion proteins are designed to interact simultaneously with two membranes during the fusion reaction ...virion membrane is primarily mediated by the transmembrane ...the ... See full document

... lipid interface in many multispanning alpha-helical membrane proteins ...this putative lipid-interactive surface of the transmem- brane region exerted no discernible effect on membrane ... See full document

... and virus membranes mediated by gp41 initiates the life cycle of human immunodefi- ciency virus type ...the ectodomain, the study of the membrane-spanning domain (MSD) has been ... See full document

... immunodeficiency virus type 1 (HIV-1) gp41 ectodomain plays a critical role in envelope glycoprotein- mediated ...peptide fusion inhibitor T20 overlap ... See full document

... the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein plays a major role in the membrane fusion step of viral ...The ... See full document

... C-terminal ectodomain remains ...and viral membranes, with its fusion peptides em- bedded in the former and its transmembrane helices anchored in the ...post- fusion trimer-of-hairpins ...the ... See full document

... differential membrane anchoring. WT and mutant env sequences, in addition to full-length tat and rev genes, were cloned into the pSRHS vector, in which expression of Env is under the control of the late ... See full document

... after viral entry, it was possible that the defect of the MSD core mutants in single-round infection could be at a postentry ...-lactamase–Vpr fusion protein (BlaM-Vpr) to pinpoint the stage at which ... See full document

... Human immunodeficiency virus type 1 (HIV-1) encodes a gp160 envelope precursor ...HIV-1 envelope gly- coprotein spikes mature by trimerization, ... See full document

... Based on the structural information about the gp41 core (4, 10, 11), it has been proposed that the binding site for the peptide inhibitors is in the HR1 bundle. The C34 and DP178 peptides presumably bind in the same way ... See full document

... prehairpin fusion interme- diate to a thermostable, six-helix bundle structure ...(Fig. 1) (reviewed in reference ...the ectodomain of gp41 self-assemble into a trimer-of-hairpins (3, 7, 17, 24, ... See full document

... the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins into assembling particles is crucial for virion ...transmembrane glycoprotein gp41 play an ... See full document

... Mixing standard amounts of reference gpl20 with increasing concentrations of human sera reduced assay sensitivity, although the linear relationship between gpl20 concentration and optica[r] ... See full document

... 463 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest The human immunodeficiency virus type 1 HIV-1 envelope glycoprotein has been shown to be extensively modified by N-li[r] ... See full document

... To examine the role of calcium ions for cell fusion mediated by interactions between CD4 and the human immunodeficiency virus type 1 HIV-1 envelope glycoprotein gpl20-gp4l, we used two e[r] ... See full document

... gp120 glycoprotein, since, in contrast to shedding, it occurs efficiently at 4°C, in the absence of the conserved stem of the V1/V2 stem-loop, and with the envelope glycoproteins from a primary HIV-1 ... See full document

... Env-mediated fusion. Treatment of PBL with M␤CD inhibited fusion medi- ated by envelope glycoproteins from HIV-1 isolates that utilize CXCR4 and CCR5, as well as chemotaxis triggered by the ... See full document

... the membrane Env trimers. Compared with the membrane Envs, the sgp140 ...immunodeficiency virus Env architecture with metastability is valid, the stabilizing changes introduced into the sgp140 ... See full document

... the envelope glycoprotein ...the envelope glycoprotein spikes on the virion ...H66N-N197S envelope glycoproteins was prepared by trans- fecting 293T ...Each virus preparation was ... See full document

... To compare the ability of different gpl20 mutants to associate with the gp4l transmembrane glycoprotein, the levels of envelope glycoproteins in the lysates and supernatants of the trans[r] ... See full document