[PDF] Top 20 A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real time PCR
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A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real time PCR
... culture DNA samples were completely concordant with post-culture DNA at all four polymorphic sites ...of time par- asites were cultured before DNA was extracted, there was no correlation ... See full document
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Development of a Real Time PCR Assay for Detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for Routine Clinical Diagnosis
... Plasmodium falciparum, P. ovale, and P. vivax—was devised and evaluated using 122 whole-blood samples from patients who had traveled to areas where malaria is endemic and who presented with ... See full document
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A real time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
... Green detection method has some ...double-stranded DNA produced during a PCR reaction, including non-specific amplification products from unrelated nucleic acids present in the sample, such as ... See full document
6
Real time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS 540 mutations in Plasmodium falciparum
... Although real-time PCR assays able to (semi)-quantify are currently available, they often do not discriminate between single and polyclonal infections ...the detection of SNPs in codon 540 of ... See full document
7
A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... A real-time PCR assay for ...the Plasmodium screening assay published by Rougemont et al for P. falciparum, ...of Plasmodium [32,33]. The assay uses primers that bind to ... See full document
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Rapid detection of Pfcrt and Pfmdr1 mutations in Plasmodium falciparum isolates by FRET and in vivo response to chloroquine among children from Osogbo, Nigeria
... and pfmdr1 polymorphisms in CQR, the Fluorescence Resonance Energy Transfer (FRET) method was used to determine these polymorphisms and their in vivo sensitivity to chloroquine in ...P. falciparum ... See full document
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Real Time Quantitative PCR for Determining the Burden of Plasmodium falciparum Parasites during Pregnancy and Infancy
... RTQ- PCR, which directly amplifies DNA from a multicopy gene without a second round of PCR, provides a more quantitative estimate of parasite burdens that blends specificity and in- creased ... See full document
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Field evaluation of the photo induced electron transfer fluorogenic primers (PET) real time PCR for the detection of Plasmodium falciparum in Tanzania
... rRNA PCR, with 100% agreement between both methods. However, PET- PCR offers important advantages over standard nested PCR, such as affordability, faster turnaround time, less chance for ... See full document
6
Real Time PCR Assay for Rapid Detection and Analysis of PfCRT Haplotypes of Chloroquine Resistant Plasmodium falciparum Isolates from India
... fields. Real-Time PCR and PfCRT haplotype ...Molecular detection of the K76T mutation associated with CQ resistance was detected by means of a standardized real-time ... See full document
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Rapid diagnostic tests as a source of DNA for Plasmodium species specific real time PCR
... isolating DNA from the proximal part of the nitrocellulose component of the RDT strip with a simple DNA elution ...The PCR on RDT showed a detection limit of ...same PCR on whole blood. ... See full document
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Sequence Based Optimization of a Quantitative Real Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
... lecular detection using PCR is becoming increasingly ...discrepant PCR and microscopy results, we aimed to optimize our detection assays for Plasmodium malariae and ... See full document
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Detection of mixed infection level of Plasmodium falciparum and Plasmodium vivax by SYBR Green I based real time PCR in North Gondar, north west Ethiopia
... Parasite DNA was extracted using a commercial kit and Plasmodium species were re-evaluated with SYBR Green I-based real time PCR to detect mixed infections and misdiagnosed ... See full document
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Molecular detection of human Plasmodium species in Sabah using PlasmoNex™ multiplex PCR and hydrolysis probes real time PCR
... diagnostic method for malaria. The invention of molecular PCR diagnostic tools can be useful for prospective and retrospective analysis of samples for surveillance and epidemiological ...available ... See full document
7
Real time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
... Real-time PCR oligonucleotide primers were manually designed for each of the genes to assure maximal effi- ciency and sensitivity, according to the following parame- ters: avoidance of the formation ... See full document
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Monitoring PfMDR1 transport in Plasmodium falciparum
... Accordingly, using Fluo-4 in drug competition assays provides a powerful tool to better examine drug trans- port kinetics via ...of PfMDR1 in drug transport, which has remained controversial for dec- ...at ... See full document
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K13 mutations and pfmdr1 copy number variation in Plasmodium falciparum malaria in Myanmar
... multiple pfmdr1 copies across the country reflects the fact that although ACT, artesunate-mefloquine and artemether-lumefan- trine, have been policy for treatment of ...P. falciparum malaria since 2005, it ... See full document
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Real Time PCR for Detection and Identification of Plasmodium spp
... assay, DNA was isolated from eight blood specimens known to contain either ...P. falciparum or ...of Plasmodium infection were again blinded and analyzed by a panel of experienced microscopists from ... See full document
6
A nested real time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
... studies using vali- dated qPCR ...of PCR product was used as the template for the second amplification to maximize the dynamic range of the assay, which allowed quantification of either submicroscopic or ... See full document
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Real Time PCR Method for Detection of Zygomycetes
... of real-time PCR in the diagnosis of zygomycete infection is the reduction of turn- around time for results from days to hours, a difference that can have a significant positive impact on ... See full document
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Artemether resistance in vitro is linked to mutations in PfATP6 that also interact with mutations in PfMDR1 in travellers returning with Plasmodium falciparum infections
... P. falciparum infection who had blood specimens submitted to the Toronto Public Health La- boratory for confirmation of ...P. falciparum, parasites were introduced into cultures and their drug sensitivity ... See full document
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