[PDF] Top 20 Modifications That Stabilize Human Immunodeficiency Virus Envelope Glycoprotein Trimers in Solution

... uble envelope glycoproteins, the radiolabeled glycoproteins were precipitated by antibodies that recognize epitopes with C1 and/or C5 components ...gp140(⫺) glycoprotein was recognized by the C11 antibody ... See full document

... Recombinant viral vaccines generally stimulate Th1-driven immune responses, likely because cytokines such as IFN- ␣ , IFN- ␥ , and Il-12 are induced by viral infection. While Th1- driven immune responses are advantageous ... See full document

... Env trimers are indistinguishable at 20-Å resolution (56, 58–60), proteolytic cleavage can influence the binding of antibod- ies to the HIV-1 Env trimer (33, 35, 48, ...Env trimers than cleaved Env ... See full document

... YU2 envelope glycoprotein variants are summarized in Table ...YU2 envelope glycoproteins elicited roughly comparable titers of antibodies reactive with the homologous YU2 gp120 glycoprotein ... See full document

... of envelope glycoprotein inhibitors have been developed as potential therapeutics against HIV-1 infection (27, 35, 44, ...HIV-1 envelope glycoprotein spikes using a 1:1 stoichiometry ...lope ... See full document

... Human immunodeficiency virus type 1 (HIV-1) infection is a significant global public health problem for which development of an effective prophylactic vaccine remains a high scientific ...viral ... See full document

... the human immune system is able to elicit this type of ...HIV-1 envelope glycoprotein (Env) trimer has further fueled the search for Env immunogens that induce bNAbs, but while native Env trimer ... See full document

... gp160 glycoprotein. This glycoprotein was expressed efficiently on the cell surface and was precipitated by antibodies that recognize conformation-depen- dent gp120 epitopes (29, ...the envelope ... See full document

... influenza virus hemagglutinin transmembrane protein HA2 ...to stabilize the gp41 ...in glycoprotein function is highlighted in a previous study that shows that mutation of Leu-550 or Leu- 561 (BH8 ... See full document

... Human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) particles typically contain small amounts of the surface envelope protein (SU), and this is ... See full document

... gp140(⫺/GCN4) glycoprotein is indeed ...GCN4) glycoprotein, which is documented to be trimeric ...gp160 envelope glycoprotein precursor and for soluble gp130 trimers (20, ... See full document

... EnvΔ712 glycoprotein bound to saturating amounts of ligands (PGT151 and soluble CD4 [sCD4]) that induce conformations other than state 1 (14, 16, 33, ...EnvΔ712 glycoprotein complexed with state ... See full document

... with virus entry may require distinct structural features in the viruses and/or recep- ...A virus, the simultaneous exposure of all HA trimers to acidic pH in the endosome facilitates the ... See full document

... homogeneous trimers (60–65, ...dimers, trimers, and higher-order aggre- gates ...SOSIP.664 trimers, all of which primarily contain high-mannose glycans (71, ...stable trimers and have been ... See full document

... other envelope proteins, with monomers, dimers, trimers, and ...surface envelope glycoproteins indicated that both gp140⌬CFI and gp145⌬CFI were synthesized and processed within ... See full document

... Env-mediated virus entry into CD4 ⴚ CCR5 ⴙ target ...and virus at 37°C for different lengths of time, the virus-compound mixtures were spinoculated onto the Cf2Th-CCR5 cells, which were further ... See full document

... To compare the ability of different gpl20 mutants to associate with the gp4l transmembrane glycoprotein, the levels of envelope glycoproteins in the lysates and supernatants of the trans[r] ... See full document

... heat-resistant virus. The pNL4.3-KB9 plasmid, which encodes the KB9 envelope glycoproteins (32) in the background of the ...and virus-containing supernatants were harvested 3 days ...of virus ... See full document

... Mixing standard amounts of reference gpl20 with increasing concentrations of human sera reduced assay sensitivity, although the linear relationship between gpl20 concentration and optica[r] ... See full document

... Although the chimpanzee that received the lowest virus dose seroconverted to p24 and gpl20 later than the animal receiving 40 cell culture infectious units, both animals had titers great[r] ... See full document