[PDF] Top 20 Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human Papillomavirus
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Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human Papillomavirus
... certain types of human papillomavirus (HPV) is a necessary risk factor for cervical cancer ...the types that are associated with the highest risk for cancer is particu- larly important (3, ... See full document
6
Development of a General Primer PCR Reverse Line Blotting System for Detection of Beta and Gamma Cutaneous Human Papillomaviruses
... HPV types that give rise to these weak signals are present in copy numbers approaching the sensitivity limit of the test ...in human skin tu- mors, there may be as little as one cutaneous HPV DNA copy per ... See full document
7
PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems
... HPV types between the MY-PCR and GP1-PCR primer ...specific types within clinical ...HPV types 53 and ...HPV types 35, 53, and 61 ...HPV types 35, 53, and 61 were ... See full document
7
Evaluation of Combined General Primer Mediated PCR Sequencing and Type Specific PCR Strategies for Determination of Human Papillomavirus Genotypes in Cervical Cell Specimens
... by PCR using  -globin ...by PCR using the very sensitive HPV PGMY09/11 general prim- ers, a more recent version of the MY09/MY11 primers ...of PCR was performed using the ... See full document
7
Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Products by a Single Hybridization, Reverse Line Blot Detection Method
... or general, primer PCR have been adopted for the major- ity of epidemiological studies (11, 20, ...of PCR methods can increase the molecular sensitivity of HPV DNA detection, the issue ... See full document
8
A general primer GP5+/GP6(+) mediated PCR enzyme immunoassay method for rapid detection of 14 high risk and 6 low risk human papillomavirus genotypes in cervical scrapings
... HPV types investi- gated in this study, provided that the OD values fall within the linear range (OD # ...of multiple HPV ...to multiple HPV ...and multiple (n 5 10) HPV ...and multiple ... See full document
5
Development of a Sensitive and Specific Assay Combining Multiplex PCR and DNA Microarray Primer Extension To Detect High Risk Mucosal Human Papillomavirus Types
... the detection and typing of human papillomaviruses (HPVs) in clinical and epidemiological studies has been well ...HPV detection and typing have been ...the detection of specific HPV ... See full document
7
MassARRAY Spectrometry Is More Sensitive than PreTect HPV Proofer and Consensus PCR for Type Specific Detection of High Risk Oncogenic Human Papillomavirus Genotypes in Cervical Cancer
... Type-specific detection of human papillomavirus (HPV) is indicated for better risk stratification and clinical management of women testing positive for HPV and for epidemiologic ...type-specific ... See full document
8
Oncogenic Human Papillomavirus (HPV) Type Distribution and HPV Type 16 E6 Variants in Two Spanish Population Groups with Different Levels of HPV Infection Risk
... on PCR amplification have been used to determine specific HPV ...of PCR products was carried out mainly by type-specific probe hybridizations and direct ...some primer sets. Thus, the PGMY09/11 ... See full document
7
Degenerate and Nested PCR: a Highly Sensitive and Specific Method for Detection of Human Papillomavirus Infection in Cutaneous Warts
... HPV types across a broad spectrum of HPV ...differential PCR methodology, despite its proven usefulness in detecting multiple HPV types within individual lesions, has a number of inherent ... See full document
11
Use of multiple PCR primer sets for optimal detection of human papillomavirus
... consensus primer sets were Oli-1b–oli-2i, My09-My11, Gp5-Gp6, and Gp5 1 -Gp6 1 from the HPV L1 gene and CpI-CpIIG from the E1 ...HPV types 11, 16, 18, 31, 33, and 35 detected more HPV-infected patients than ... See full document
6
Two novel genital human papillomavirus (HPV) types, HPV68 and HPV70, related to the potentially oncogenic HPV39
... FIG. 3. Nucleotide sequence alignments of the 3 9 end of the long control region, the E6 and E7 ORFs (A), and the L1 ORF (B) of HPV68 and -70 DNAs. The initiation and stop codons of the E6, E7, and L1 ORFs are ... See full document
7
Evaluation of FRET real time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
... the detection of Plasmodium ...real-time PCR technology may preclude its use in resource-poor clinics, its performance character- istics, combined with its rapid results, suggest that it may be a useful ... See full document
11
Detection of Human Papillomavirus DNA by PCR in Esophageal Squamous Cell Carcinoma from Turkmen Sahra, North-East of Iran
... 85 human squamous cell carcinomas 53 men and 32 women, aged between 42 to 85 years were removed by biopsy between 1997 to 1998 from Turkmen Sahra and were studied in the Institute of Public Health and Research at ... See full document
5
Comparison of the Roche AMPLICOR MYCOBACTERIUM assay and Digene SHARP Signal System with in house PCR and culture for detection of Mycobacterium tuberculosis in respiratory specimens
... in-house PCR are not ...of PCR were present in the clinical ...ture-positive PCR-negative specimens were negative by all the three PCR methods, suggesting that inhibitors may have been ... See full document
5
Rapid detection of human papillomavirus in cervical scrapes by combined general primer mediated and type specific polymerase chain reaction
... A two-step polymerase chain reaction PCR procedure was used as a new screening strategy for the detection of human papillomavirus HPV genotypes in cervical scrapes omitting prior DNA ext[r] ... See full document
5
Comparison of Linear Array and Line Blot Assay for Detection of Human Papillomavirus and Diagnosis of Cervical Precancer and Cancer in the Atypical Squamous Cell of Undetermined Significance and Low Grade Squamous Intraepithelial Lesion Triage Study
... the detection of persistent infection will be a spe- cific marker of risk that may be used clinically to distinguish persistent infections from transient infections that pose little ...HPV detection for ... See full document
9
A highly sensitive modified nested PCR to enhance case detection in leishmaniasis
... fully sensitive diagnostic tool to detect leishmaniasis while enabling the exclusion of Crithidia ...complicate detection of Leishmania ...another PCR tech- nique with high sensitivity and ...of ... See full document
10
Detection of Multiple Human Papillomavirus Types in Condylomata Acuminata Lesions from Otherwise Healthy and Immunosuppressed Patients
... the PCR assay in our study amplifies and detects HPV in genital samples with better sensitivity than other ...The PCR and reverse blot strip assay employed in our study used different primer pools ... See full document
7
Rapid and Sensitive Detection of Physical Status of Human Papillomavirus Type 16 DNA by Quantitative Real Time PCR
... Real-time PCR assay for clinical specimens with cervical ...real-time PCR assay, and the presence of con- comitant forms was considered in cases displaying E2/E6 copy number ratios below ...real-time ... See full document
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