[PDF] Top 20 Negative Autoregulation of Epstein-Barr Virus (EBV) Replicative Gene Expression by EBV SM Protein

... by SM (47, 61), these results indicate that SM could actually enhance accumulation of BHRF1 mRNA posttranscriptionally, similar to its effect on the major- ity of EBV transcripts ...of SM on ... See full document

... observed expression of the BARF1 gene localized in the BamHI A fragment of the viral genome ...BARF1 gene, encoding a 31-kDa early protein, has been shown to be able to induce malignant ... See full document

... on EBV lytic gene expression and ...infectious EBV virion production, we per- formed further experiments to investigate which steps of the viral lytic cycle were affected by DHX9 ...lytic ... See full document

... of Epstein-Barr virus (EBV) gene expression and cross-linking of its surface immunoglobulin G (IgG) by antibodies results in activation of viral ...When EBV nuclear ... See full document

... Moreover, the EBV-infected Blymphoma cells are often partially permissive for early replicative cycle gene expression and virus replication can be induced, in contrast to previously repo[r] ... See full document

... function. SM-mediated gene activation is correlated with enhanced cy- toplasmic accumulation of intronless target gene mRNAs (7, ...39). SM contains an LRR resembling an NES found in certain ... See full document

... STAT1 expression by alternative cleavage and polyadenylation has been well de- scribed, and several lines of evidence indicate that the ratio of STAT1 ␤ to STAT1 ␣ modulates the cellular response to viral and ... See full document

... of gene expression by KS-SM. (A) BJAB cells (from an EBV-negative Burkitt lymphoma) were cotransfected with 10 ␮g of either KS-SM expression plasmid or control plasmid and ... See full document

... a negative control, whereas primers for known EBNA3A/3C binding sites at the ADAM28/ADAMDEC1 intergenic enhancer (ADAM peak) were used as positive controls of EBNA3A/3C ...-tubulin protein expression ... See full document

... that expression of a number of late lytic genes is stimulated by DNA replication alone in the absence of ...of EBV in that their expression was enhanced by DNA replication per ...by SM (shown ... See full document

... in EBV-immortalized lymphoblastoid ...of EBV-immortalized B cells (721, RPMI-1788, JO-L-B1, 11/17-1, 11/17-3, and 11/17-10) and in BJAB cells transfected with ...EBNA-1-mediated expression of ... See full document

... by SM and were poorly expressed in its absence. We also compared gene expression between WT and SMKO viruses after induction of lytic replication as shown in ...lytic gene expression ... See full document

... for gene speci- ficity of SM-mediated activation. Because SM had clearly dis- tinguishable effects on the accumulation of different mRNAs, we wished to determine whether the efficiency of mRNA bind- ... See full document

... inducible SM-expressing cell line. To study the effects of SM on host cell gene expression, we gen- erated a cell line that inducibly expresses SM upon treatment with 4-hydroxytamoxifen ... See full document

... IE protein ICP27 (35). Interestingly, ex- pression of the HSV ICP27 gene can substitute for M in an in vitro EBV ori-Lyt replication assay (7), suggesting a functional ...cellular protein ... See full document

... for EBV early genes was defined as 5=- T-A-T-A-A ⬎ t-A-3=, whereas a consensus TATT box sequence for EBV late genes was defined as 5=- ...BcRF1 protein onto the late core ...viral gene ... See full document

... after EBV infection compared to the levels in seronegative controls ( ⬎ ...toms. EBV gp350 is the most abundant virus envelope protein in virions and binds to its receptor CD21 on B cells, ... See full document

... Salient features of the clones used Clone pSL93 pSL77 pM-B2-K pM-B2-C pGD4 pMC9 pDH33 pM-B2-J pPL9 pPL10 pPL12 pMC14 pTS6 pDH123 pGH47 pMC31 EBV sequences BamHI-C BamHI-H BglII-K BgII-C [r] ... See full document

... and virus replication was ...reduced expression of BZLF1 and activation of p38 ...the protein to FIG 2 Expression of BGLF2 induces p38 and JNK ...BGLF2 protein with a V5 epitope tag, a ... See full document

... RR1 protein levels remain constant throughout the cell cycle ...for virus replication in tissue culture cells (7, ...since expression of the entire gene was ...and EBV RR, which results ... See full document