[PDF] Top 20 Nonstructural proteins of Semliki Forest virus: synthesis, processing, and stability in infected cells.
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Nonstructural proteins of Semliki Forest virus: synthesis, processing, and stability in infected cells.
... The markers for A: a ts-1induced proteins labeled for 1 min in isotonic medium after 30 min treatment with 335 mM NaCl and chased for 60 min; b ts-1-induced proteins labeled with the win[r] ... See full document
11
Differences in Processing Determinants of Nonstructural Polyprotein and in the Sequence of Nonstructural Protein 3 Affect Neurovirulence of Semliki Forest Virus
... genetic stability of rescued recombinant ...ns proteins, revealed that the localization of ns proteins in BHK-21 cells infected with SFV6 ...ns proteins in ... See full document
16
Properly Folded Nonstructural Polyprotein Directs the Semliki Forest Virus Replication Complex to the Endosomal Compartment
... RNA synthesis in alphavirus-infected cells, generating plus-strand RNAs, takes place on cytoplas- mic vacuoles (CPVs), which are modified endosomes and ...individual Semliki Forest ... See full document
12
Furin Processing and Proteolytic Activation of Semliki Forest Virus
... TBE virus demonstrated that proteolytic maturation of the prM protein was mediated by furin and required prior exposure of the virus to mildly acidic pH (21, ...TBE virus buds into the endoplasmic ... See full document
9
Studies on defective interfering Semliki Forest virus
... DI virus on virus-polypeptide synthesis in co-infected cells also showed that different DI virus preparations behaved ...DI virus preparations p6 and pl2e abrogated the ... See full document
295
Cleavage Map and Proteolytic Processing of the Murine Norovirus Nonstructural Polyprotein in Infected Cells
... during virus replication, we prepared total lysates of MNV-infected (MOI of 2 to 4) cells at 4-h time intervals ...of proteins at various times, with detection at 12 h p.i. and peak ... See full document
16
Enzymatic Defects of the nsP2 Proteins of Semliki Forest Virus Temperature-Sensitive Mutants
... Sindbis virus (SIN; a related alphavirus) nsP2 have verified that the carboxy-terminal half contains the autoprotease activity of the protein (9, 18), for which a conserved cysteine (Cys 478 in SFV) and histidine ... See full document
12
Semliki Forest Virus Nonstructural Protein 2 Is Involved in Suppression of the Type I Interferon Response
... RNA synthesis is accompanied by translational shutoff, possi- bly aiding the immediate cessation of cellular gene expression (1, ...this virus also causes a general inhibi- tion of cellular RNA ... See full document
8
Early synthesis of Semliki Forest virus-specific proteins in infected chicken cells.
... At the times after superinfection with SFV indicated on the abscissa, cells pretreated with FPV and actinomycin D were pulse labeled for 15 min with 25 pCi of tritiated amino acids per 1[r] ... See full document
12
Molecular Determinants of Substrate Specificity for Semliki Forest Virus Nonstructural Protease
... Semliki Forest virus (SFV) is an enveloped positive-strand RNA virus of the genus Alphavirus, family ...Alpha- virus replication relies on the production of replicase proteins in ... See full document
10
Control of protein synthesis in Semliki forest virus-infected cells.
... The viral structural proteins were detectable with certainty at 3.5 h postinfection, and their rate of synthesis increased linearly parallel to the amount of their messenger, the 26S RNA[r] ... See full document
8
Tryptic peptide analysis on nonstructural and structural precursor proteins from Semliki Forest virus mutant-infected cells.
... DISCUSSION The accumulation of large nonvirion proteins in cells infected with temperature-sensitive mutants of Semliki Forest virus allowed analysis of their tryptic peptides and compar[r] ... See full document
15
Replication of Semliki Forest virus RNA in infected chick cells
... Estimation of the time required to synthesize a molecule of viral RNA As explained earlier Discussion, Section III 1, if RNA is being synthesized and labelled at a constant rate, the lab[r] ... See full document
177
Nuclear localization of Semliki Forest virus-specific nonstructural protein nsP2.
... 1888-1896 0022-538X/90/051888-09$02.00/0 Copyright C 1990, American Society for Microbiology Nuclear Localization of Semliki Forest Virus-Specific Nonstructural Protein nsP2 JOHAN PERANE[r] ... See full document
9
Sites of synthesis of viral proteins in avian sarcoma virus-infected chicken cells.
... 629 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We determined the sites of synthesis of avian sarcoma virus-specific proteins in infected chicken cells by immunopre[r] ... See full document
8
Mechanism for Control of Synthesis of Semliki Forest Virus 26S and 42S RNA
... The inhibition of 26S RNA synthesis in ts-4 mutant-infected cells shifted to 390C; the concomitant loss of RNase sensitivity of the minus strand in RIs that previously could be cleaved t[r] ... See full document
9
Electron Microscopy of Cells Infected with Semliki Forest Virus Temperature-Sensitive Mutants: Correlation of Ultrastructural and Physiological Observations
... Electron Microscopy of Cells Infected with Semliki Forest Virus Temperature-Sensitive Mutants: Correlation of Ultrastructural and Physiological Observations Department of Microbiology, J[r] ... See full document
7
Human MxA Protein Confers Resistance to Semliki Forest Virus and Inhibits the Amplification of a Semliki Forest Virus-Based Replicon in the Absence of Viral Structural Proteins
... HEp-2 cells were chosen because they are susceptible to many viruses and do not ex- press endogenous MxA ...The cells were cotransfected with pHMG-MxA (27) and pSV2-neo (36) and subsequently selected in the ... See full document
7
Proteins synthesized by Semliki Forest virus and its 16 temperature-sensitive mutants.
... In this paper we show that variable amounts of five nonvirion proteins with molecular weight between 60,000 and 130,000 accumulate at the restrictive temperature in the cells infected by[r] ... See full document
10
Identification of virus-coded nonstructural polypeptides in bunyavirus-infected cells.
... Analyses of SSH virus-infected cell extracts and extracts of cells infected with SSH-LAC reassortants having SSH medium Msize RNA species indicated that a nonstructural polypeptide NSM; [r] ... See full document
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