[PDF] Top 20 Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications
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Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications
... L-115 amplification and electrophoresis onto microchips could lead to NASBA on Chips for quick and accurate identification of disease producing genes at the patient’s bed ...side. NASBA has all the ... See full document
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Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay
... serotype-specific NASBA methods de- tected dengue viral RNA in 66 samples, for a sensitivity of ...group-specific NASBA assay that was applied to a subset of the 67 ...serotype-specific NASBA assay ... See full document
5
Development of a Nucleic Acid Sequence Based Amplification Assay That Uses gag Based Molecular Beacons To Distinguish between Human Immunodeficiency Virus Type 1 Subtype C and C′ Infections in Ethiopia
... during NASBA amplification. Fig- ure 1a demonstrates the amplification of a subgroup C virus and clearly shows the recognition of the amplicon by the bea- con homologous to the C sequences but not by ... See full document
8
Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification
... Bacterial strains. The bacterial strains used to test the specificity of the NASBA primers are presented in Table 1. Mycoplasma strains were cultured in spiroplasma (SP4) medium (40) without thallium acetate ... See full document
7
Evaluation of the NucliSens Basic Kit for Detection ofChlamydia trachomatis and Neisseria gonorrhoeaein Genital Tract Specimens Using Nucleic Acid Sequence Based Amplification of 16S rRNA
... of NASBA was determined by test- ing serial dilutions of freshly prepared stock cultures of ...total nucleic acids with the Basic Kit guanidinium/silica extrac- tion protocol, which extracts both DNA and ... See full document
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Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens
... Real-time NASBA. Real-time mono NASBA reactions were performed using the NucliSens Basic Kit amplification module (bioMe ´rieux) as described previ- ously (18, 19, ...target nucleic ... See full document
7
Sensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis
... Material and Methods: First, the RNA was extracted from 28 skin biopsies suspected cutaneous Leishmaniasis. Then, by means of specific primers designed for 18srRNA region, this region was amplified using NASBA ... See full document
7
Improved Detection of Rhinoviruses by Nucleic Acid Sequence Based Amplification after Nucleotide Sequence Determination of the 5′ Noncoding Regions of Additional Rhinovirus Strains
... isolates. NASBA using primer mix 1 and probe combinations I and III classified 80 of 88 ...for amplification and detection (Fig. 1). We were unable to sequence isolates M0107266 and M2107365 because ... See full document
6
RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples
... internal NASBA standard will prevent false-negative ...that NASBA is a sensitive and reliable amplifi- cation ...by NASBA and PCR is in agreement with those obtained in other studies investigating ... See full document
7
Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae
... pneumoniae amplification assays. For isothermal nucleic acid sequence-based amplification (NASBA), 16S rRNA has obvi- ously been used as a target, and for PCR, the 16S ... See full document
6
Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification
... species. Based on the analytical evaluation of the developed test, it was concluded that the sensitivity of the developed tests is ...developed NASBA for ...are based on the number of in vitro RNA ... See full document
6
Construction Strategy for an Internal Amplification Control for Real Time Diagnostic Assays Using Nucleic Acid Sequence Based Amplification: Development and Clinical Application
... molecular amplification-based methods is an internal amplification control (IAC), which should be included in each reaction ...nontarget nucleic acid sequence which is ... See full document
6
Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St Louis Encephalitis Viruses
... assay, amplification reactions were set up by combining 5 l of RNA with 50 pmol of each primer in a 10-l amplification ...90-min amplification reaction, a 5-l portion was removed and was diluted ... See full document
8
Analytical Performance and Clinical Utility of a Nucleic Acid Sequence Based Amplification Assay for Detection of Cytomegalovirus Infection
... A nucleic acid sequence-based amplification (NASBA) assay for qualitative detection of human cytomegalo- virus (CMV) pp67 mRNA was evaluated in a multicenter ...mRNA ... See full document
6
Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence Based Amplification
... EV NASBA performed very well during the study period, detecting all 31 positive CSF samples, including 10 CSF sam- ples that were culture ...EV NASBA results were observed, and one sample containing ... See full document
5
Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification
... methods based on RT-PCR have been described (25, 31, 32, ...amplified. NASBA has been described as an alter- native approach to specifically detect RNA targets and is based on the activity of avian ... See full document
6
Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season
... RT-PCR-positive, NASBA-nega- tive specimens as rhino- or enteroviruses, the 5 ⬘ NCRs of 38 strains displaying a band at 380 bp on agarose gel that were RT-PCR positive, NASBA negative and of 9 strains with ... See full document
6
Prevalence of Plasmodium spp in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification
... is based on a overproduction of polyclonal IgM antibodies, leading to accumulation of high molecular-weight immu- nocomplexes and complement ...18S NASBA positivity; ... See full document
7
Value of Different Assays for Detection of Human Cytomegalovirus (HCMV) in Predicting the Development of HCMV Disease in Human Immunodeficiency Virus Infected Patients
... by nucleic acid sequence-based amplifica- tion (NASBA) was ...RT-PCR, NASBA allows direct isothermal amplification of specific se- quences of single-stranded RNA ...by ... See full document
7
Diagnostic Value of Monitoring Human Cytomegalovirus Late pp67 mRNA Expression in Renal Allograft Recipients by Nucleic Acid Sequence Based Amplification
... by nucleic acid sequence-based amplification (NASBA) after renal-allograft transplantation was ...specific amplification of the late pp67 (UL65) mRNA. NASBA results ... See full document
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