[PDF] Top 20 Null Mutations in the lin-31 Gene Indicate Two Functions During Caenorhabditis elegans Vulval Development
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Null Mutations in the lin-31 Gene Indicate Two Functions During Caenorhabditis elegans Vulval Development
... of vulval cell transcription factors appear to play important roles fates and typically fall into two broad phenotypic classes, in many developmental processes, including establish- vulvaless (Vul) or ... See full document
8
EGL 38 Pax regulates the ovo related gene lin 48 during Caenorhabditis elegans organ development
... indicated lin-48 requires the Pax gene egl-38 for its expression in hindgut cells (see below), we mutated this site in a reporter that included sequences to 4697, and found that the mutant transgenes fail ... See full document
9
Structural Requirements for the Tissue-Specific and Tissue-General Functions of the Caenorhabditis elegans Epidermal Growth Factor LIN-3
... generate vulval cell fate specification, cell growth, division, and survival, progeny, although all six have the potential to do ...(18) vulval fate, and the two in one ...of lin-3 as ... See full document
13
Caenorhabditis elegans lin-13, a Member of the LIN-35 Rb Class of Genes Involved in Vulval Development, Encodes a Protein With Zinc Fingers and an LXCXE Motif
... ob- lin-13 activity. Furthermore, lin-13 mRNAs containing served among the progeny of dsRNA-injected hermaph- such early premature stop codons are likely to be unsta- rodites raised at ...C. elegans ... See full document
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lin-8, Which Antagonizes Caenorhabditis elegans Ras-Mediated Vulval Induction, Encodes a Novel Nuclear Protein That Interacts With the LIN-35 Rb Protein
... the LIN-8 family: The non- sense alleles n2731 and n2738 appeared to be null alleles on the basis of their molecular lesions and lack of LIN-8 protein, yet, by comparison to loss of the class A ... See full document
15
Pathway to RAS
... controlling development (K auffman 1973; T homas ...C. elegans were lin-12 (G reenwald et al. 1983), lin-14 (A mbros and H orvitz 1984), and tra-1 (H odgkin 1983), controlling space, time, and ... See full document
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A Multipartite Approach to Mapping the Gene Network Directing Caenorhabditis elegans Vulval Organogenesis
... of vulval develop- ment. Two categories of transcription factors are relatively more difficult to identify in genetic ...screens. Mutations that result in lethality or other severe defects will not ... See full document
141
Identification of Residues of the Caenorhabditis elegans LIN-1 ETS Domain That Are Necessary for DNA Binding and Regulation of Vulval Cell Fates
... 1.—LIN-1 gene and pro- tein. (A) The lin-1 locus is dia- grammed with exons (numbered boxes), introns (lines), and the start (ATG) and stop (TAG) codons of the open reading frame (Beitel et ...in ... See full document
14
Isoform-Specific Mutations in the Caenorhabditis elegans Heterochronic Gene lin-14 Affect Stage-Specific Patterning
... the LIN-14 isoforms do not perform distinct at performing the stage-specific functions of ...If LIN- common region of the LIN-14 proteins is also sufficient 14A is more efficient at specifying ... See full document
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Caenorhabditis elegans lin-45 raf Is Essential for Larval Viability, Fertility and the Induction of Vulval Cell Fates
... Raf functions in signal transmission by phosphorylating and activating a mitogen- activated protein (MAP) kinase kinase named ...13 mutations in the Caenorhabditis elegans lin-45 raf ... See full document
12
Caenorhabditis elegans lin-25: A Study of Its Role in Multiple Cell Fate Specification Events Involving Ras and the Identification and Characterization of Evolutionarily Conserved Domains
... experiments, two different for induction of the 1⬚ fate (Miller et ...how lin-25 might func- et al. 1995), and LIN-1 and LIN-31 proteins are both tion during VPC fate ...First, ... See full document
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Protruding Vulva Mutants Identify Novel Loci and Wnt Signaling Factors That Function During Caenorhabditis elegans Vulva Development
... pvl-6 gene product, nor do we know in what cell pvl-6 subtle effect of this lin-26 mutation, it has been found ...the lin-26(ga91) mutation represents a missense mutants, it is possible that there is ... See full document
20
Stage-Specific Timing of the microRNA Regulation of lin-28 by the Heterochronic Gene lin-14 in Caenorhabditis elegans
... of lin-28’s 39 UTR reveals that in addition to one lin-4 family binding site, there is at least one conserved let-7 family binding ...that lin-28 is regulated by let-7 miRNAs, but this hypothesis has ... See full document
17
Identification of Heterochronic Mutants in Caenorhabditis elegans: Temporal Misexpression of a Collagen::Green Fluorescent Protein Fusion Gene
... of lin-42 ...stage lin-42(ve16) ...stage lin-42(ve20) hermaphrodite stained with the antibody ...stage lin-42(ve20) showing precocious col-19::gfp expres- ... See full document
17
Programmed Cell Death During Caenorhabditis elegans Development
... egl-1 gene and the pig-1 gene, which encodes an AMPK-like protein kinase most similar to the mammalian kinase MELK and which, as discussed above (see The Q lineage), is a component of a genetic pathway that ... See full document
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Promotion of oogenesis and embryogenesis in the C elegans gonad by EFL 1/DPL 1 (E2F) does not require LIN 35 (pRB)
... cej-1) (Jones and Schedl, 1995; Lee and Schedl, 2001). We have generated an expanded list of candidate GLD-1 targets by identifying mRNAs that co-immunoprecipitate with GLD-1 and probing microarrays (M.-H. Lee, V.R. and ... See full document
12
Caenorhabditis elegans unc-37/groucho Interacts Genetically With Components of the Transcriptional Mediator Complex
... porter gene in wild-type male ...disease gene (Barr and Sternberg 1999) and is expressed in all nine rays as well as ray axons ...unc-37 null (unc-37[wd17dm wd22]) ...reporter gene in an ... See full document
5
The SUMO system in Caenorhabditis elegans development
... cellular functions that participate in developmental processes such as cell division, cell fate decisions, differentiation and ...different functions, while a single gene encodes for SUMO in yeast ... See full document
7
Necessity and Contingency in Developmental Genetic Screens: EGF, Wnt, and Semaphorin Pathways in Vulval Induction of the Nematode Oscheius tipulae
... nematode Caenorhabditis elegans identified the EGF/Ras and Notch pathways as central for vulval precursor cell fate ...(1°) vulval fate; ...1° vulval fates. Here, we report on the ... See full document
16
LIN 12/Notch trafficking and regulation of DSL ligand activity during vulval induction in Caenorhabditis elegans
... of LIN-12 in all VPCs, suggesting that at least some of these serine/threonine residues are likely to be constitutively phosphorylated to promote basal ...of LIN-12 from the apical plasma membrane in all ... See full document
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