[PDF] Top 20 Performance Characteristics of the QUANTIPLEX HIV 1 RNA 3 0 Assay for Detection and Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
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Performance Characteristics of the QUANTIPLEX HIV 1 RNA 3 0 Assay for Detection and Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... for quantitation of HIV-1 RNA in ...and detection methods, including reverse tran- scription (RT) followed by PCR and colorimetric detection (RT-PCR assay; Roche Molecular ... See full document
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Evaluation of Performance Characteristics of the Aptima HIV 1 Quant Dx Assay for Detection and Quantitation of Human Immunodeficiency Virus Type 1 in Plasma and Cervicovaginal Lavage Samples
... of HIV-1 RNA has been an established surrogate marker for monitoring viral suppression and drug efficacy during antiretroviral therapy (ART) in HIV-infected ...the HIV-1 ... See full document
6
Comparative evaluation of NASBA HIV 1 RNA QT, AMPLICOR HIV monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... quantitating HIV-1 RNA levels in plasma were evaluated for their ability to accurately measure viremia levels in clinical ...in HIV-1 baseline reactivity were observed between ... See full document
7
Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... of plasma human immunodeficiency virus type 1 (HIV-1) RNA levels has become an important tool for identifying individuals likely to benefit from ... See full document
6
Highly Sensitive Methods for Quantitation of Human Immunodeficiency Virus Type 1 RNA from Plasma, Cells, and Tissues
... RT-PCR. RNA extracted with silica columns was analyzed by the Amplicor HIV-1 Monitor ...QIAamp-extracted plasma RNA (50 ml that already con- tained QS) was mixed with 50 ml of the ... See full document
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Evaluation of a quality assurance program for quantitation of human immunodeficiency virus type 1 RNA in plasma by the AIDS Clinical Trials Group virology laboratories
... bDNA assay (Chiron assay; Chiron Corporation, ...the HIV-1 polymerase ...signal. Detection occurs by the addition of a chemiluminescent substrate and quantitation of the relative ... See full document
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Intra Assay Performance Characteristics of Five Assays for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... Fig. 1 that there was little or no difference between the nominal HIV-1 RNA concentrations and the es- timates of HIV-1 RNA concentration obtained by the Chiron, NGI, BCM, ... See full document
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Multicenter Evaluation of the Performance Characteristics of the NucliSens HIV 1 QT Assay Used for Quantitation of Human Immunodeficiency Virus Type 1 RNA
... be HIV-1 positive were evalu- ated with both the NucliSens and the NASBA ...NASBA assay is 400 copies/ml with a 1-ml specimen input volume compared to 25 copies/ml for the NucliSens ...with ... See full document
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Quantitative Detection of Plasma Human Immunodeficiency Virus Type 2 Subtype A RNA by the Nuclisens EasyQ Assay (Version 1 1)
... one assay originally de- veloped and already approved for use for the measurement of the viral load in HIV-1 carriers may be equally valid for the quantification of HIV-2 viremia is of ... See full document
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Comparative Performance of Three Viral Load Assays on Human Immunodeficiency Virus Type 1 (HIV 1) Isolates Representing Group M (Subtypes A to G) and Group O: LCx HIV RNA Quantitative, AMPLICOR HIV 1 MONITOR Version 1 5, and Quantiplex HIV 1 RNA Version 3 0
... of human immunodeficiency virus type 1 (HIV-1) infection and implementation of treatment strate- gies rely on the quantitative measurement of HIV-1 ... See full document
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Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV 1) RNA bDNA v3 0, Cobas AmpliPrep/Cobas TaqMan HIV 1, and NucliSens HIV 1 EasyQ v1 2, Testing HIV 1 Non B Subtypes and Recombinant Variants
... [URFs; 3 A/D and 4 U/C]) defined at pol and obtained from 49 HIV-1-infected patients were selected for the ...83 plasma specimens were collected from 20 subjects receiving highly active ... See full document
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Application of a commercial kit for detection of PCR products to quantification of human immunodeficiency virus type 1 RNA and proviral DNA
... Plasma HIV-1 RNA sample ...The RNA extraction reagent was prepared by mixing ...of plasma was added to each ...2,900 3 g for 10 min at 4 8 C, resulting in the separation ... See full document
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Correlation between Human Immunodeficiency Virus Type 1 (HIV 1) RNA Measurements Obtained with Dried Blood Spots and Those Obtained with Plasma by Use of Nuclisens EasyQ HIV 1 and Abbott RealTime HIV Load Tests
... of detection for HIV RNA in DBS by either of the two commercial assays was greater than ...log HIV RNA ...of plasma viremia of greater than 3 log units while a patient is ... See full document
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Impact of Human Immunodeficiency Virus Type 1 (HIV 1) Genetic Diversity on Performance of Four Commercial Viral Load Assays: LCx HIV RNA Quantitative, AMPLICOR HIV 1 MONITOR v1 5, VERSANT HIV 1 RNA 3 0, and NucliSens HIV 1 QT
... Human immunodeficiency virus type 1 (HIV-1) evolution and changing strain distribution present a chal- lenge to nucleic acid-based ...the detection and accurate ... See full document
9
Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: application to acute retroviral infection
... A dilution series between 20 and 120 copies of the pCC2 HIV-1 transcript RNA 0, the pNAS2 quantitation standard transcript RNA [1 and HIV-1 genomic RNA A was each amplified for 26, 27, a[r] ... See full document
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Quantification of Genital Human Immunodeficiency Virus Type 1 (HIV 1) DNA in Specimens from Women with Low Plasma HIV 1 RNA Levels Typical of HIV 1 Nontransmitters
... genital HIV-1 DNA in specimens from women with low plasma HIV-1 RNA ...assess HIV-1 DNA levels in endocervical and vaginal secretions in relation to plasma ... See full document
6
Quantitation of Human Immunodeficiency Virus Type 1 (HIV 1) RNA in Cell Free Cervicovaginal Secretions: Comparison of Reverse Transcription PCR Amplification (AMPLICOR HIV 1 MONITOR 1 5) with Enhanced Sensitivity Branched DNA Assay (Quantiplex 3 0)
... 30 HIV-1- seropositive women and from 30 HIV-seronegative healthy women not at risk for ...for 3 days before en- rollment. Samples were processed within 1 h of ...with 3 ml of ... See full document
5
HIV Load Testing with Small Samples of Whole Blood
... to human immunodeficiency virus (HIV) viral load (VL) testing is of paramount importance for the success of antiretroviral therapy treatment campaigns throughout the ...with HIV from ... See full document
7
Quantitation of Human Immunodeficiency Virus Type 1 RNA in Different Biological Compartments
... available HIV RNA assays: Roche’s Ampli- cor HIV-1 Monitor (reverse transcriptase PCR [RT-PCR]), Organon-Teknika’s nucleic acid sequence-based amplification (NASBA), and Chiron’s signal ... See full document
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Evaluation of the Abbott LCx HIV 1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA
... (Chiron Quantiplex 3.0). Samples were processed by the LCx HIV RNA Quantitative ...the assay controls and clinical samples were ex- tracted by centrifugation and separation using a QIAAmp col- ... See full document
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