[PDF] Top 20 Processing of the gp55-116 envelope glycoprotein complex (gB) of human cytomegalovirus.

... From these results, the intracellular processing of the gp55-116 complex appears to involve N-linked glycosylation of the Mr 105,000 nonglycosylated precursor to an abundant glycosylated[r] ... See full document

... of human cytomegalovirus (HCMV), which is considered essential for the viral life cycle, is proteolytically processed during ...in human fibroblasts, showing growth kinetics comparable to that of ... See full document

... Consistent with the findings in the influenza virus system 28, we also report that for CMV gB the amino acid sequence at the site of cleavage does affect the susceptibility of the molecu[r] ... See full document

... gCIII complex forms is ...gCIII complex forms in the ER, immunoprecipitate of pulse-chase samples with the anti-gO antibody were digested with endo H, which cleaves high-mannose N-linked glycans from the ... See full document

... 58, 1986 using SYNTHESIS OF CMV ENVELOPE gp55-116 COMPLEX one-dimensional proteolytic peptide mapping of the mature virion forms of these proteins also indicate that gp55 ACKNOWLEDGMENTS[r] ... See full document

... A comprehensive understanding of the structure-function relationship of any HCMV glycoprotein is lacking. gB plays a vital role in many aspects of the viral life cycle, including entry, cell-to-cell spread, and ... See full document

... However, the functional significance of this disulfide bond and the tight loop that it likely creates is unknown. An in-frame, four-amino-acid insertion mutant six amino acids downstream of C250 (mutant I-256) resulted ... See full document

... carbohydrate processing of gpTRL10 when it was expressed in the absence of other viral ...HCMV envelope glycoproteins, gpTRL10 was complexed with other viral pro- teins, most likely through disulfide bonds, ... See full document

... Our results suggested that postoligomerization folding of gB was necessary for transport from the ER and that the half-maximal time of formation of this form was esti6747 Downloaded from[r] ... See full document

... 3309 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The human cytomegalovirus HCMV envelope glycoprotein complex gp55-116 was expressed in both Escherichia coli and ce[r] ... See full document

... glycan processing associated with transit from the ER through the Golgi complex), these data are consistent with the notion that pentamer formation leads to an increase in protein maturation and surface ... See full document

... Because the HHV-6A U100 gene product is predicted to contain 11 potential N-linked glycosylation sites, we next ex- amined whether the U100 gene products were glycosylated; this was done by treatment with endo H, which ... See full document

... Human cytomegalovirus (HCMV) infection is widespread and usually without symptoms in healthy adults, but it can cause severe disease in the immunologically immature or im- munodeficient host and is a ... See full document

... The partial inhibition of the neutralizing activity of monoclonal antibody 7-17 by the nonneutralizing antibody 27-287 was not unexpected, because both antibodies inhibited one another i[r] ... See full document

... immune complex FIG. 5. Neither soluble gQ-Fc alone nor gH-gL complex alone binds to ...the complex-bound beads were incubated with CD46(309his) (panels a and b, lanes 2) or CD4(387his) (panels a and ... See full document

... The initial rate of viral glycoprotein synthesis was unaffected in gro29 cells, but the extent of export from the endoplasmic reticulum to the Golgi apparatus was impaired and export thr[r] ... See full document

... Cell lines and reagents. H9 cells persistently infected with molecularly cloned virus NL4-3 (1) have been described elsewhere (29, 30). Cell lines were main- tained in RPMI 1640 (Gibco/BRL, Bethesda, Md.) containing 10% ... See full document

... multaneously to alanine. The respective amino acids within AD-4 were identified using the three-dimensional (3D) model of gB as shown in Fig. 1B, and this prediction was used to design 19 poten- tial candidate pairs for ... See full document

... Antibodies. Monoclonal antibodies AP86, 27-78, 27-39, 7-17, and 14-4b were provided by W. Britt and have been described previously (3, 8, 10, 22, 56). Polyclonal 6824 and anti-UL74 have also been described previously ... See full document

... virus encoding gE that cannot undergo endocytosis (37). Mod- erate reduction in virus replication for gB of HSV-1 and de- velopment of a small-plaque phenotype in cultured cells for gE of PRV have been associated with ... See full document