[PDF] Top 20 Properties of cells transformed by the middle T-antigen-coding region of polyomavirus.
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Properties of cells transformed by the middle T-antigen-coding region of polyomavirus.
... The serum independence, saturation density, and anchorage independence of cell lines producing no detectable amounts of large T antigen or fragments thereof vary over a range which overl[r] ... See full document
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Transformation by Polyomavirus Middle T Antigen Involves a Unique Bimodal Interaction with the Hippo Effector YAP
... was transformed into Stbl3 competent cells (Invitrogen) propagated at ...293FT cells (Invitrogen) for lenti- viral packaging ...NMuMG cells, cells were exposed overnight to a 1:4 ... See full document
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A Transformation-Defective Polyomavirus Middle T Antigen with a Novel Defect in PI3 Kinase Signaling
... a region rich in proline ...this region of MT is responsible for binding a host target protein that contains a Src homology domain 3 (SH3) ...in cells and is unable to activate PI3K-mediated pathways ... See full document
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Phosphatidylinositol metabolism in cells transformed by polyomavirus middle T antigen.
... We did not find consistent changes in total inositol phosphate levels or in the levels of Ins1,4P2 or Ins1,4,5P3 which, when normalized to the level of PtdIns present in cells, correlate[r] ... See full document
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Production of novel polyphosphoinositides in vivo is linked to cell transformation by polyomavirus middle T antigen.
... Although PI-3-P is found in both growing and confluent nontransformed and transformed fibroblasts, PI-3,4-P2 and PIP3 are only detected in PyMTAg-transformed cells, growing cells, and PD[r] ... See full document
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Tiny T antigen: an autonomous polyomavirus T antigen amino-terminal domain.
... tiny T antigen raises the question as to whether it has a separate function in productive infection and transfor- mation of ...tiny T antigen is responsible for some of the activities ascribed ... See full document
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Evidence of a Role for Phosphatidylinositol 3-Kinase Activation in the Blocking of Apoptosis by Polyomavirus Middle T Antigen
... to middle T antigen in F111 cells transformed by ...serum-starved cells. Akt activity, shown in Fig. 5, was roughly twofold higher in cells transformed by wild-type ... See full document
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Transformation and tumorigenic properties of a mutant polyomavirus containing a middle T antigen defective in Shc binding.
... that middle T antigen is sufficient to induce full transformation in established cell lines (44, 55), while large T antigen is necessary for immortalization of pri- mary cells ... See full document
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Amino-terminal regions of polyomavirus middle T antigen are required for interactions with protein phosphatase 2A.
... 293 cells were transfected with plasmids encoding the various MT proteins, and immune complex kinase assays were carried out after isolation of MT from cell ...DKGG region had wild-type levels of associated ... See full document
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Polyomavirus Middle T-Antigen Is a Transmembrane Protein That Binds Signaling Proteins in Discrete Subcellular Membrane Sites
... Rat2 cells were transfected with plasmids containing MT and mutant DNA using a calcium phosphate ...the cells were then fed every 3 days for 14 days, and the foci were stained by incubation with Leishmann’s ... See full document
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Polyomavirus Middle T Antigen Induces the Transcription of Osteopontin, a Gene Important for the Migration of Transformed Cells
... the region within the OPN promoter responsive to MT, we used OPN-luc reporter constructs containing various ...the region between ⫺ 258 and ⫺ 88 contains the response elements primarily responsible for MT ... See full document
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Regulation of pp60c-src and its interaction with polyomavirus middle T antigen in insect cells.
... To determine whether i pp6Oc-src produced in insect cells was enzymatically active, ii MTAg could associate with any endogenous insect tyrosine kinases, and iii MTAg and pp60-csrc would [r] ... See full document
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The p110α Isoform of Phosphatidylinositol 3-Kinase Is Essential for Polyomavirus Middle T Antigen-Mediated Transformation
... human cells of two distinct cell ...epithelial cells, in agree- ment with published data for rodent fibroblasts, expression of Y250F was fully transforming in both human epithelial cells and ...rine ... See full document
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Multimerization of polyomavirus middle-T antigen.
... mutant middle-T antigen were transiently or stably expressed in NIH 3T3 ...expressed T antigens. As shown in Fig. 2A, full-length middle-T antigen was specif- ically ... See full document
6
Isolation and characterization of NIH 3T3 cells expressing polyomavirus small T antigen.
... DISCUSSION By introducing the small T-antigen gene into cells with a retrovirus vector as replication-defective retrovirus, we were able to isolate NIH 3T3 cells expressing only polyomav[r] ... See full document
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Association of p60fyn with middle tumor antigen in murine polyomavirus-transformed rat cells.
... As assessed by immune-complex protein kinase assays, the ability of the FYN3 antiserum to immunoprecipitate rat p601'"" could be prevented by preincubating the antiserum with FYN peptide[r] ... See full document
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Antibodies against a nonapeptide of polyomavirus middle T antigen: cross-reaction with a cellular protein(s).
... However, unexpected results were obtained when middle T antigen that phosphorylated in in vitro MT kinase reac- and Table 1 establish that the 55K protein immunoprecipitated from wild-ty[r] ... See full document
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Localization of the phosphorylations of polyomavirus large T antigen.
... Hydroxylamine cleavage of large T antigen labeled with [35S]methionine yielded peptides that matched electrophoretically the two phosphorylated fragments.. A third fragment 60K fragment [r] ... See full document
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Relationship between T-antigen and tumor-specific transplantation antigen in simian virus 40-transformed cells.
... 1 Relationship Between T-Antigen and TumorSpecific Transplantation Antigen in Simian Virus 40Transformed Cells Received for publication 18 July 1978 The simian virus 40 SV40 tumor antige[r] ... See full document
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The DNA-binding properties of polyomavirus large T antigen are altered by ATP and other nucleotides.
... In the absence of ATP and with increasing quantities of Py TAg, sites A and B as well as site C, for which the data are not shown, on the early side of the origin palindrome, were protec[r] ... See full document
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