[PDF] Top 20 Protein 2A is not required for Theiler's virus replication.
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Protein 2A is not required for Theiler's virus replication.
... the 2A protein of Theiler’s virus does not have any obvious identity with the 2A proteases of poliovirus and rhinovirus, with protein 2A of hepatitis A virus, or even with ... See full document
8
Epstein-Barr virus nuclear protein 2A forms oligomers in vitro and in vivo through a region required for B-cell transformation.
... The plasmid pGAD424, which encodes the activation domain present in amino acids 768 to 881 of GAL4, was used to generate fusion proteins containing those segments of EBNA-2 present in th[r] ... See full document
8
Binding Interactions between the Encephalomyocarditis Virus Leader and Protein 2A
... of 2A (first 50 amino acids) and the central hinge region of ...displace 2A from ...encephalomyelitis virus (TMEV; BeAn) and Saffold virus interact similarly in any subtype combination, with ... See full document
7
The CD225 Domain of IFITM3 Is Required for both IFITM Protein Association and Inhibition of Influenza A Virus and Dengue Virus Replication
... and/or protein localization. IFITM3’s NTD (amino ac- ids 1 to 50) was fused in frame with IFITM1’s amino acids 29 to 245 or IFITM2’s amino acids 50 to 118 to generate M3M1 and M3M2 ...of ... See full document
16
The Cellular Protein SPT6 Is Required for Efficient Replication of Human Cytomegalovirus
... mutant virus and expression plasmid was sequenced to confirm the specific point mutation and rule out the possibility of a secondary mutation elsewhere in the ...viral replication, UL69 shuttling activity, ... See full document
10
Conserved GXXXG- and S/T-Like Motifs in the Transmembrane Domains of NS4B Protein Are Required for Hepatitis C Virus Replication
... The incompatibility in the TMD region does not affect NS4B subcellular distribution or processing. In Huh7.5 cells, NS4B protein displays foci when expressed alone or in the context of the viral genome (3, 44, 48, ... See full document
16
Functional Properties of the Human Cytomegalovirus IE86 Protein Required for Transcriptional Regulation and Virus Replication
... IE86 protein is essential for HCMV replication due to its ability to transactivate critical viral early ...glutathione S-transferase (GST) pulldown assays were performed, re- vealing that Y544 is ... See full document
10
Identification of Residues Required for RNA Replication in Domains II and III of the Hepatitis C Virus NS5A Protein
... of replication in the context of the Con1/SG-neo S2204I replicon, mutations were subcloned from Con1/Sg-neo GIT or pSL1180GIT into a replicon plasmid harboring the S2204I ... See full document
11
The FUSE Binding Protein Is a Cellular Factor Required for Efficient Replication of Hepatitis C Virus
... We also used a HCV subgenomic replicon-containing lucif- erase reporter (Luc-HCV RNA) (51) to determine the effect of FBP overexpression on HCV translation. We had two par- allel sets of cured MH14 cells transfected with ... See full document
13
The Major Site of Phosphorylation within the Rous Sarcoma Virus MA Protein Is Not Required for Replication
... immunodeficiency virus [HIV] MA [14, 15]) might also exist in RSV ...a protein tyrosine kinase, based on its surrounding sequences which have characteristics of known tyrosine phosphorylation sites ...be ... See full document
5
Interactome Analysis of the Influenza A Virus Transcription/Replication Machinery Identifies Protein Phosphatase 6 as a Cellular Factor Required for Efficient Virus Replication
... lie virus replication. During infection, the influenza virus tran- scription/replication machinery makes numerous interactions with the host cell (reviewed in references 10, 73, and ... See full document
16
Glycine Zipper Motifs in Hepatitis C Virus Nonstructural Protein 4B Are Required for the Establishment of Viral Replication Organelles
... asterisks. Residues targeted in this study are underlined and highlighted in red. (C) Helical wheel representations of NS4B TMS2 (left) and TMS3 (right) are depicted. Bulky hydrophobic amino acids are shown in blue, ... See full document
20
Coat Protein Regulation by CK2, CPIP, HSP70, and CHIP Is Required for Potato Virus A Replication and Coat Protein Accumulation
... used. Protein phosphatase 2A (PP2A) gene with PP2A Forward primer (5= GAC CCT GAT GTT GAT GTT CG 3=) and PP2A Reverse primer (5= GAG GGA TTT GAA GAG AGA TTT C 3=) and F-box protein gene with F-box ... See full document
14
Modifications to the Foot-and-Mouth Disease Virus 2A Peptide: Influence on Polyprotein Processing and Virus Replication
... that 2A can act as a translational regulator to modify the amounts of the different parts of the polyprotein that are ...the 2A peptide is located at the boundary between the upstream capsid proteins and ... See full document
16
Plum Pox Virus 6K1 Protein Is Required for Viral Replication and Targets the Viral Replication Complex at the Early Stage of Infection
... FIG 4 The loss-of-infectivity phenotype of PPV 6K1 deletion mutants cannot be rescued by the trans expression of 6K1 or P3-6K1 through transient expression or the cis expression of a dislocated 6K1 in the modified PPV ... See full document
13
Ectromelia virus RING finger protein is localized in virus factories and is required for virus replication in macrophages.
... (Fig. 2A through C), Mac-1 or F4/80 antibody (not shown), and Hoechst dye ...early virus-specific protein, as shown by the anti-E3L antibody staining ...mutant virus-infected macrophages that ... See full document
9
Palmitoylation of the Influenza A Virus M2 Protein Is Not Required for Virus Replication In Vitro but Contributes to Virus Virulence
... HA protein for virus replication in vitro, palmitoylation of the M2 protein appears to be dispensable for replication in a number of different cell types and culture ...reassortant ... See full document
7
Aichi Virus 2A Protein Is Involved in Viral RNA Replication
... nonstructural 2A protein varies among vi- ruses in amino acid sequence and ...rhinoviruses, 2A is a protease responsible for cleavage of the polyprotein at its own N terminus (14, ...poliovirus ... See full document
5
Encephalomyocarditis Virus 2A Protein Is Required for Viral Pathogenesis and Inhibition of Apoptosis
... ⌬ 2A-infected cells and controls (STS treatment or mock) are represented in ...wild-type virus (MOI of 5) for 8 and 14 h, 72 and 89% of cells were infected (that is, positive for VP1), respectively, and the ... See full document
14
Peroxiredoxin 1 Is Required for Efficient Transcription and Replication of Measles Virus
... A luciferase gene was inserted between the N and P genes in MeV-luc; however, the extent of reduction of luc mRNA ex- pression was greater than that of P mRNA and similar to that of L mRNA (Fig. 6). Furthermore, the ... See full document
7
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