[PDF] Top 20 Protein covalently bound to minus-strand DNA intermediates of duck hepatitis B virus.
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Protein covalently bound to minus-strand DNA intermediates of duck hepatitis B virus.
... MASON Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Analysis of duck hepatitis B viral DNA by gel electrophoresis, Southern blotting, and bindi[r] ... See full document
8
Mutations within DR2 independently reduce the amount of both minus- and plus-strand DNA synthesized during duck hepatitis B virus replication.
... hepadnaviral DNA synthesis are the coencapsidation of the pregenomic RNA and viral P protein (1, 2, 10, 21) and initiation and synthesis of the first four nucleotides of minus-strand ... See full document
7
Analysis of Duck Hepatitis B Virus Reverse Transcription Indicates a Common Mechanism for the Two Template Switches during Plus-Strand DNA Synthesis
... plus-strand DNA. Synthesis of plus-strand DNA begins after completion of synthesis of minus-strand ...Full-length minus-strand DNA immediately prior to the ... See full document
7
The Majority of Duck Hepatitis B Virus Reverse Transcriptase in Cells Is Nonencapsidated and Is Bound to a Cytoplasmic Structure
... core protein (data not shown), eliminating the possibility that polymerase detected by immunoprecipitation was derived from immature cores that were detergent sensi- ...core protein versus polymerase not ... See full document
10
Initiation and termination of duck hepatitis B virus DNA synthesis during virus maturation.
... To map the termination sites of minus-strand DNA synthesis, virion DNA was digested with AccI and 5' end labeled at the AccI sites, one not RESULTS Evidence that protein is linked to the[r] ... See full document
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T5 Exonuclease Hydrolysis of Hepatitis B Virus Replicative Intermediates Allows Reliable Quantification and Fast Drug Efficacy Testing of Covalently Closed Circular DNA by PCR
... replicative intermediates are present, which could replenish and amplify the intracellular “cccDNA pool” ...(e.g., duck hepatitis B virus [DHBV]), where numbers of 50 cccDNA molecules ... See full document
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cis-Acting sequences in addition to donor and acceptor sites are required for template switching during synthesis of plus-strand DNA for duck hepatitis B virus.
... viral DNA isolated from the cytoplasmic capsids of trans- fected LMH ...viral DNA detected by primer extension was measured relative to a DNA standard that was added to the viral DNA samples ... See full document
9
n-Butyrate, a cell cycle blocker, inhibits early amplification of duck hepatitis B virus covalently closed circular DNA after in vitro infection of duck hepatocytes.
... DHBV DNA extracted as described in Materials and ...(A) DNA recovered from the soluble fraction (cccDNA fraction); (B) DHBV protein-bound replicative ...DHBV DNA (100 pg) was ... See full document
7
Characterization of the Intracellular Deproteinized Relaxed Circular DNA of Hepatitis B Virus: an Intermediate of Covalently Closed Circular DNA Formation
... circular DNA (cccDNA) of hepatitis B virus (HBV) is formed by conversion of capsid- associated relaxed circular DNA (rcDNA) via unknown mechanisms and exists in the nucleus of the ... See full document
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Half-Life of the Duck Hepatitis B Virus Covalently Closed Circular DNA Pool In Vivo following Inhibition of Viral Replication
... pathogen Hepatitis B virus (HBV) and animal viruses such as Duck hepatitis B virus (DHBV) and Woodchuck hepatitis B virus (WHV), which are important ... See full document
8
Insertions within epsilon affect synthesis of minus-strand DNA before the template switch for duck hepatitis B virus.
... of DNA synthesis at the wild-type position, we de- termined the site of initiation of minus-strand DNA synthesis by primer ...the DNA synthesis reaction in vitro, a fraction of ... See full document
10
RNA sequences controlling the initiation and transfer of duck hepatitis B virus minus-strand DNA.
... nascent minus-strand DNA need not be hybridized to the ...2-nt DNA-RNA hybrids are unlikely to be sufficiently ...mature minus-strand DNA with a 5 9 end at nt 2538 is ... See full document
9
A novel transcriptional element in circular DNA monomers of the duck hepatitis B virus.
... enveloped DNA vi- ruses that include the human hepatitis B virus, woodchuck hepatitis virus (WHV) (27), ground squirrel hepatitis virus (19), and several avian ... See full document
6
PCR mediated recombination impacts the analysis of hepatitis B Virus covalently closed circular DNA
... To investigate sensitivity, we performed real-time SYBR Green PCR. Serum S1 (viral load of 8.42 log IU/ ml) was digested with DNase I, serially diluted and the Ct determined. There was a good linear correlation between ... See full document
5
Duck Hepatitis B Virus Virion Secretion Requires a Double-Stranded DNA Genome
... ss DNA, full-length ss DNA (but little or no ds DNA), and finally, the mature ds ...viral DNA detectable in the culture medium, with or without the additional 2-day period of virion ... See full document
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Duck hepatitis B virus polymerase acts as a suppressor of core protein translation.
... Immunoprecipitation. Fifty microliters of each in vitro-translated product was incubated on ice with 150 m l of RIPA buffer (150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate [SDS], and ... See full document
8
Multiple functions of capsid protein phosphorylation in duck hepatitis B virus replication.
... On the other hand, aspartic acid substitution of serines 257 and 259 caused specific reductions in enveloped virus production, suggesting that phosphorylation at these sites would be exp[r] ... See full document
8
Signals for Bidirectional Nucleocytoplasmic Transport in the Duck Hepatitis B Virus Capsid Protein
... a protein of 30 kDa ...core protein amino acids, expected to abrogate capsid assembly (43), yielded a predominantly nuclear fluores- cence ...core protein amino acid ...panel B. (B) ... See full document
10
Secondary Structure of the 3′ Terminus of Hepatitis C Virus Minus-Strand RNA
... (⫺) strand RNA to chemical and enzymatic probes, using two in vitro-transcribed RNA ...(⫺) strand nucleotides was the same as that of the (⫹) strand, except that they were preceded by a minus ... See full document
11
A Structural Model for Duck Hepatitis B Virus Core Protein Derived by Extensive Mutagenesis
... HBc protein and of assem- bled HBV capsids is known in detail from biochemical (4, 26, 27, 36) and biophysical (46) investigations of recombinant HBV capsid-like particles ...c/e1 B-cell epitope (3, 11, ... See full document
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