[PDF] Top 20 Proteomics profiling of urine with surface enhanced laser desorption/ionization time of flight mass spectrometry
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Proteomics profiling of urine with surface enhanced laser desorption/ionization time of flight mass spectrometry
... with only 8% and 24% for CHCA and SPA, respectively. This indicates that for urine the Q10 chip has a relative large variation in signal to noise ratios between spots compared to other chip types. The percentages ... See full document
9
Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein profiling
... and mass spectrometry (MS)-based clinical proteomics has become a popular method to both streamline drug discovery and provide specific diagnostic biomarkers and surrogate end points for a number of ... See full document
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Proteomic biomarkers predicting lymph node involvement in serum of cervical cancer patients. Limitations of SELDI-TOF MS
... Material & methods: Serum samples of 60 cervical cancer patients (FIGO I/II) were obtained before primary treatment. Samples were run through a HPLC depletion column, eliminating the 14 most abundant proteins ... See full document
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The new platinum-based anticancer agent LA-12 induces retinol binding protein 4 in vivo
... and time- course studies using mass-spectrometry-based analysis to measure the proteomic profiles of rat plasma in response to LA-12 and compared them with the recent pharmacokinetic data ...the ... See full document
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Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... matrix-assisted laser desorption ionizationⴚtime of flight mass spectrometry (MALDI-TOF MS) profiling is efficient for arthro- pod identification at the species ...before ... See full document
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Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... cases, in contrast to the remaining CoNS, whose microbiological relevance was never ⬎26%. The 50 S. lugdunensis isolates selected here for microbiological analysis originated from various inde- pendent sampling sites: ... See full document
7
Mass determination of major plasma proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... for profiling the relative amounts of different proteins would require extensive investigation of appropriate dilu- tions, the linearity, and calibration of responses, and standardization of ... See full document
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... impaired by inhibitory compounds (9), requiring specific ex- traction and purification strategies. MS involves no enzymatic step, so purification is much less of an issue. The products of nucleic acid amplification serve ... See full document
7
The discovery and identification of a candidate proteomic biomarker of active tuberculosis
... of proteomics technology makes the analysis of all the proteins in the serum ...possible. Surface- enhanced laser desorption ionization time of flight mass ... See full document
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Rapid identification of medically important mosquitoes by matrix assisted laser desorption/ionization time of flight mass spectrometry
... protein profiling, it is deemed necessary that the integrity of protein is maintained during collection, transporta- tion, storage and processing of the ...with time as more data is accumulated in this ... See full document
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Prospective Evaluation of a Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench by Bench Study for Assessing the Impact on Time to Identification and Cost Effectiveness
... cultures; urine cultures; stool cultures; aerobic wound cultures; an- aerobic wound and tissue cultures; respiratory tract cultures; and cystic fibrosis respiratory tract ... See full document
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Direct Identification of Urinary Tract Pathogens from Urine Samples, Combining Urine Screening Methods and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... direct urine samples is a process for which stan- dardization of the procedure is ...three urine processing methods (data not shown), es- tablishing 2 ml as the optimum volume, as it is easily manipulated ... See full document
6
Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid
... high mass accuracy and high resolution MS technology is necessary before this approach can be used as a more comprehen- sive proteomic profiling tool in an automated and high throughput ... See full document
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Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... bacterial mass (approxi- mately 10 l) was suspended in 300 l of Milli-Q water using a mi- cropestle, and the suspension was vortex-mixed for 30 ...rial mass at 100°C for 30 min in a water bath ... See full document
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Identification of Highly Pathogenic Microorganisms by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial
... H ighly pathogenic bacteria (HPB) are risk group 3 bacteria, which are defined as biological agents that can cause severe human disease and present a serious hazard to health care work- ers. This may present a risk of ... See full document
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Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... turnaround time, abro- gating one of the main advantages of MALDI-TOF ...processing time to as little as 3 to 5 min, reduced consumables, and eliminated extra specimen label- ing and manipulation ... See full document
8
Application of MALDI TOF MS to rapid identification of anaerobic bacteria
... of flight mass spectrometry (MALDI- TOF MS) has been widely used in clinical microbial diagnosis in the past ...rapid mass spectrometry technology developed in the late 1980s with ... See full document
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Evaluation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates
... Matrix-assisted laser desorption ionization– time of flight (MALDI-TOF) has been recently applied to the identification of a wide range of microorganisms (2), mainly clin- ically ... See full document
5
Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS
... The yeast identification was accomplished by means of MALDI-TOF MS Biotyper RTC software, version 3.0 (Bruker Daltonics), based on the comparison of mass spectra generated by the Microflex LT software with ... See full document
5
Evaluation of the Andromas Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Aerobically Growing Gram Positive Bacilli
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a rapid bacterial identification technique that is increasingly used in ... See full document
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