[PDF] Top 20 Quantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
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Quantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
... detected DNA from C. albicans, the most common Candida species, by LightCycler PCR and melting curve ...in PCR-based diagnostic methods for C. albicans. Standardized PCR ... See full document
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Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real Time PCR Assay
... (HCMV) DNA in blood is becoming the standard method for monitoring HCMV infection in immune-suppressed and unsuppressed ...various blood compart- ments can be used, we have compared the HCMV ... See full document
6
Quantification by real time PCR of Trypanosoma cruzi DNA in samples of Triatoma infestans used in xenodiagnosis of chronic Chagas disease patients
... human blood to triatomine vector de- pends on the specie used in the XD [41, 47], the develop- ment of one clonal genotype from mixed infection can be inhibited [43] and that the most important strains belong- ing ... See full document
6
A nested real time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
... Blood samples in this study were obtained during passive and active malaria surveillance visits (occurring every two weeks) in Kalifabougou from May 2011 to December 2011 as previously described ... See full document
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Clinical Predictive Value of Real Time PCR Quantification of Human Cytomegalovirus DNA in Amniotic Fluid Samples
... by PCR of CMV DNA in maternal blood at the time of amniocentesis was ...in blood was not carried out at the time of the prenatal diagnosis for any of the three mothers from our ... See full document
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Quantification of Leishmania infantum DNA by a Real Time PCR Assay with High Sensitivity
... A real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to reach a sensitivity of ...comparative PCR by using the Leishmania DNA ... See full document
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Comparison of Different Methods of Isolation of DNA of Commonly Encountered Candida Species and Its Quantitation by Using a Real Time PCR Based Assay
... as real-time PCR assay has been reported as an alternative to conventional culture for early detection of invasive ...the DNA extraction step still requires more than half of a working ...that ... See full document
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... clinical samples collected from febrile neutropenic ...tested using enzyme- linked immunosorbent assays, and Aspergillus fumigatus and Candida albicans deoxyribonucleic acid (DNA) ... See full document
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Validation of a pan orthopox real time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
... quantitative real time PCR assay validated for the determination of monkeypox viral GC levels in cynomolgus macaque EDTA whole blood (Table ...near- real-time monitoring of viral ... See full document
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Real Time PCR Quantification of Human Cytomegalovirus DNA in Amniotic Fluid Samples from Mothers with Primary Infection
... disease, quantification of HCMV DNA in plasma or leukocytes has been proposed to be more specifically associated with the dis- ease in immunocompromised patients (6, ...the quantification of HCMV ... See full document
6
Development of a TT Virus DNA Quantification System Using Real Time Detection PCR
... TTV DNA levels among HCV-infected patients and blood ...the samples from HCV-infected patients with elevated ALT levels and those with normal ALT levels and voluntary blood donors, the serum ... See full document
5
Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan Based PCR Assay
... C. albicans ribosomal DNA (rDNA) loads in blood samples by using the TaqMan technology combined with the GeneAmp 5700 real-time sequence detection system (Applied ... See full document
6
Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... plasma samples obtained from patients at the 302 Military Hospital of China and group B consisted of 59 plasma samples obtained from patients at Beijing ChaoYang Hospital between July 2016 and Au- gust ... See full document
9
Quantification of CDR1 Gene Expression in Fluconazole Resistant Candida Glabrata Strains Using Real-time PCR
... clinical samples in hospitals of Te- hran in 2015 and initially, obtained isolates were identified by phenotypic methods such as colony on Candida Chromagar Medium (CHROMagar Company, Paris, ...formed ... See full document
5
Quantification of Feline Herpesvirus 1 DNA in Ocular Fluid Samples of Clinically Diseased Cats by Real Time TaqMan PCR
... FeHV-1 DNA content from a given infectious dose, and vice ...fluid samples from cats experimentally infected with FeHV-1 and specific FeHV-1-free ...quantitative PCR was observed, although only early ... See full document
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Binding and entry of peste des petits ruminants virus into caprine endometrial epithelial cells profoundly affect early cellular gene expression
... different time points, the cells were washed with phos- phate-buffered saline (PBS) and treated with proteinase K (2 mg/mL) (Solarbio, China) for 45 min at 4 °C to remove the adsorbed but not internalized ... See full document
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Original Article Prognostic significance of circulating plasma Epstein-Barr virus DNA in monitoring aggressive non-Hodgkin’s lymphoma
... EBV DNA parameters demonstrated that it was an independent poor prognostic factor for OS in patients with ...EBV DNA load may be a useful potential biomarker and prognostic indicator in ... See full document
8
Exon duplications in the ATP7A gene: Frequency and Transcriptional Behaviour
... RT-PCR using primer-pairs flanking the duplications (Table ...control samples, and the difference in size corresponds to the expected size of the duplica- ... See full document
11
Development and Application of Real Time PCR Assay for Quantification of Mycobacterium ulcerans DNA
... assay. Real-time PCR has been used to mon- itor the efficacy of antimalarial (15), antibacterial (2), and antiviral (17, 31) ...of DNA quantified in sputum corresponded well with the num- bers ... See full document
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The design and evaluation of a shaped filter collection device to sample and store defined volume dried blood spots from finger pricks
... of DNA recovered from DBS using the Chelex procedure compared to whole blood subject to Qiagen ...chromosomal DNA released by Chelex extraction of a 20 μl blood spot was less than 7% of ... See full document
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