[PDF] Top 20 Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... to DNA showing that 70% of the protein-DNA contacts occur through these three nucleotides (4, ...Our analysis also indicated that the nature of the first residues has little effect on ...viral ... See full document
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Discrete regions of simian virus 40 large T antigen are required for nonspecific and viral origin-specific DNA binding.
... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... upon binding to DNA, or dimerizes only weakly, but rather binds primarily in an independent manner to each TBS under our assay ...independent binding of two OBD molecules to two adjacent inverted ... See full document
7
Four major sequence elements of simian virus 40 large T antigen coordinate its specific and nonspecific DNA binding.
... By testing the effects of single-site mutations on virus replication and by performing a number of DNA-binding assays on replication-negative T-antigen mutants, we have identified severa[r] ... See full document
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Nonspecific DNA binding activity of simian virus 40 large T antigen: evidence for the cooperation of two regions for full activity.
... Second, wild-type T antigen's nonspecific DNA binding, but not its origin binding, was significantly inhibited with a number of monoclonal antibodies PAb1O4, PAb114, PAb252, PAb4O2, PAb4[r] ... See full document
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Simian Virus 40 Large T Antigen Disrupts Genome Integrity and Activates a DNA Damage Response via Bub1 Binding
... pRB binding was required, since other viral onco- proteins have been shown to induce the DDR via this pathway ...pRB binding deficient (via a K1 mutation) ...lar DNA replication (4, 17, ...Bub1 ... See full document
11
Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ... See full document
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The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document
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Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document
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DNA-binding activity of simian virus 40 large T antigen correlates with a distinct phosphorylation state.
... i They clearly show the relationship between different subclasses, i.e., the DNAbinding, monomeric nuclear high-salt fractions on one hand and the nonbinding, oligomeric fractions on the[r] ... See full document
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The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... two DNA sequences, as well as the reactions that take place at the two sites, are ...that T antigen interacts with these two regions of the origin ... See full document
7
Specific mutation of a regulatory site within the ATP-binding region of simian virus 40 large T antigen.
... Although we already knew that the SV2905 genome was defective for viral DNA replication in vivo, we tested the purified 2905T in an SV40 DNA replication assay in vitro 48.. Our assay was[r] ... See full document
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... the T-ag-obd onto the X-ray structure of pap- illomavirus E1 obd complexed to DNA, as models generated by these alignments are in the helicase-proximal orientation (see ...the T-ag obd onto the E1 ... See full document
9
Multiple DNA Damage Signaling and Repair Pathways Deregulated by Simian Virus 40 Large T Antigen
... of simian virus 40 (SV40) large T antigen (LT), without a viral origin, is sufficient to induce the hallmarks of a cellular DNA damage response (DDR), such as focal ... See full document
14
Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
... papovavirus simian virus 40 (SV40) minichromosome has been used extensively as a model to study the mechanism of eukaryotic DNA replication (see references 8 and 24 and references ... See full document
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Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document
8
Functional analysis of a simian virus 40 super T-antigen.
... We have not compared the properties of the super and large T-antigens in the maintenance of transformation, but noted that cells transformed by the super T-antigen templates and the C120[r] ... See full document
9
Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... of DNA replication in eukaryotes have been productively studied by using simian virus 40 (SV40) (reviewed in references 17 and ...SV40 DNA replication requires a single viral factor, ... See full document
8
Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... The T-Ag OBD is connected to the helicase domain by a flexible linker, and hence much of the surface of the T-Ag OBD hexamer remains solvent accessible even in the context of full-length ...the T-Ag ... See full document
10
Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen.
... Nine monoclonal antibodies reacted with determinants located in region I, because the antibodies precipitated small t as well as large T antigens from extracts of SV40-transformed or -in[r] ... See full document
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