[PDF] Top 20 Quantitative Expression and Virus Transmission Analysis of DC-SIGN on Monocyte-Derived Dendritic Cells

... of DC- SIGN expression on the MDDCs derived from our donor pool precluded us from drawing conclusions about DC-SIGN ex- pression levels and virus transmission ... See full document

... THP-1/huDC- SIGN cells with MAb 11C1 reduced luciferase activity in the recipient cells to near-background levels ...ptDC- SIGN containing deletions of the neck or CRD ...293T cells ... See full document

... 4A). Analysis of qRT-PCR results demonstrated that mVP40-Ci67 and mVP40-Ravn significantly decreased IFN- ␤ mRNA expression, although the decrease in induction was quite modest at all time points ... See full document

... of dendritic cells (DCs) in human immunodeficiency virus (HIV) transmission at mucosal surfaces, we examined the expressions of the HIV adhesion molecule, dendritic-cell-specific ICAM-3 ... See full document

... these cells to support HIV-1 replication ...the virus and spread it in an infectious form, contrasting in this way with other HIV-1 target cells, like CD4 ⫹ T lymphocytes or macrophages ... See full document

... facilitate virus capture and trans- mission to CD4 ⫹ T cells (33, 37). DC-SIGN is the only C-type lectin receptor identified so far that mediates trans-enhance- ment of T-cell infection, an ... See full document

... of DC-SIGN on B-THP-1 cells leads to produc- tive ...the DC-SIGN-mediated enhanced binding of HTLV-1 to B-THP-1/DC-SIGN cells, we proceeded to determine whether ... See full document

... for expression of a late HSV-1 protein, gD, by flow cytometry and confocal microscopy at time points ranging from 12 to 48 h ...mock-infected cells and isotype-matched antibody controls. DC stained ... See full document

... mononuclear cells from leukapheresis were cultured for 7 days in serum-free VacCell medium (Invitrogen) supple- mented with 500 U of granulocyte-macrophage colony-stimulating factor (No- vartis, France)/ml and 50 ... See full document

... reassess DC-SIGN functions. Most results described so far on the role of DC-SIGN relied on the use of blocking antibodies and mannan (22, ...of DC-SIGN for its ligand compared to ... See full document

... Vpx-containing virus-like particles (VLPs) generated as described previously by Negre et ...⫹ cells) were evaluated by fluorescence-activated cell sorting (FACS) ...or DC-SIGN- expressing HEK ... See full document

... For analysis of cell-surface marker expression by flow cytometry, we incubated DCs for 10 min with normal mouse serum and then 30 min with fluorochrome-conju- gated anti-human monoclonal ...ochem); ... See full document

... B). Expression of MxA was induced in both infected and FIG 2 Vpx enhances HIV-1 transmission from T cells to ...T cells) were labeled with the cell tracker CMFDA and cocultured with target ... See full document

... RNA expression of target genes was measured in stimulated and nonstimulated mMOs and mDCs with the ABI Prism 7500 Sequence Detection System (Ap- plied ...pression, cells were tested at 0 and 8 h after ... See full document

... Dendritic cells (DCs) are the directors of the immune sys- tem and form the messengers between the innate and the adaptive immune ...T cells can differentiate into a variety of effector subsets, ... See full document

... tumor cells were dispersed to create a single-cell suspension. The cells were lysed by four to five freeze cycles (on liquid nitrogen) and thaw cycles (room ... See full document

... h, cells were stained for sCPXV012 peptide and either LAMP1, TAP1, or pp65 (Figure ...by dendritic cells and partially colocalized with LAMP1 and ... See full document

... of DC differentiation protocol in experimental ...of DC-based immune treatment against HIV-1 [15] it is interesting to remember that the in vitro manipulation of DC could be a limited step in vaccine ... See full document

... production, DC matu- ration (CD83 expression), or the expression level of the co- stimulatory molecule ...ICAM-1 expression on the DC, which is an important factor for DC- ... See full document

... Dendritic cells (DCs) play an essential role in immunity and are used in cancer ...these cells can be tuned by tumors with immunosuppressive responses. DC-specific intercellular adhesion ... See full document