[PDF] Top 20 Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis by Pyrosequencing Technology

... The technology is also increasingly assessed for species identifica- tion or typing of various microbes (21), including mycobacteria ...Quantitative Pyrosequencing analysis of linezolid resis- tance in ... See full document

... is rapid and not ...of detection, only short hybridization reactions are required with target sequences amplified by bpHDA to generate detectable surface-bound ... See full document

... M. tuberculosis complex and to probe for mutations within the RRDR of the rpoB ...M. tuberculosis rpoB primers and rpoB-specific molecular beacons were modified from those described previously (11) to allow ... See full document

... M. tuberculosis isolates. A total of 243 isolates of M. tuberculosis from patients from north India and Mexico were tested for mutations associated with resis- tance to rifampin by the molecular ... See full document

... known resistance mutation is an artifact of subculture on artificial ...M. tuberculosis and screening for rifampin resistance ...by resistance ratio and proportional drug susceptibility ... See full document

... and rifampin (RIF), with or without resistance to other anti-TB ...with resistance to any fluoroquinolone and to at least one of three injectable second-line drugs (kanamycin, capreomy- cin, or ... See full document

... of tuberculosis (TB) in devel- oped as well as developing countries since 1980 has been as- sociated with the HIV epidemic, the emergence of drug-resis- tant strains, and increases in emigration from regions with ... See full document

... of Mycobacterium tuberculosis is an increasing problem for populations and tu- berculosis control programs in developed and developing countries ...Today, rifampin (RMP) and isoniazid (INH) are ... See full document

... as rapid diagnostic tests for detection of drug resistance ...The technology combines PCR with subsequent reverse ...metric detection reaction resulting in banding patterns suggestive ... See full document

... Mycobacterium tuberculosis remains one of the most significant causes of death from an infectious ...agent. Mycobacterium tuberculosis complex bacteria and resistance to rifampicin drug ... See full document

... detecting rifampin (RIF) and isoniazid (INH) resistance in Mycobacterium tuberculosis in an accurate, affordable, and rapid ...M. tuberculosis clinical isolates of known ... See full document

... Mycobacterium tuberculosis is one of the leading causes of death worldwide, and multidrug-resistant tuber- culosis (MDR-TB) is associated with a high case fatality ...rate. Rapid identification of ... See full document

... the rapid and specific detection of the most-frequent mutations leading to IHN and RMP resistance in clinical ...M. tuberculosis isolates by applying an easy-to-perform test ...PCR ... See full document

... high-level resistance (62, ...the detection of the most important mutations which are associated with resistance to rifampin and isoniazid as well as to ...MDR-TB detection and cannot ... See full document

... the detection of rpoB mutations (5–7, 9, 11, ...the detection of RIF ...the rapid and specific detec- tion of the most frequent mutations leading to INH and RIF resistance in clinical ...the ... See full document

... Xpert has been noted to have a number of limitations. Despite its excellent sensitivity in tests of smear-positive sputum samples, Xpert is somewhat less sensitive when testing smear-negative sputum. For example, when ... See full document

... M. tuberculosis complex and Mycobacterium avium complex strains present in full-grown cultures in the BACTEC MGIT 960 culture system was made with the aid of the Accuprobe system (Gen-Probe ...M. ... See full document

... For RIF, the PSQ assay yielded valid results for 118 specimens; DST results were available for 114 specimens because of a lack of growth in four specimens. Three DST results were excluded from analysis. One specimen had ... See full document

... conferring resistance to RIF, INH, and OFX, ...a rapid, accurate, and cost-effective method possessing high sensi- tivity and specificity for the determination of antibiotic resistance among MTB ... See full document

... Since detection of drug resistance in M. tuberculosis by clas- sical culture methods requires 6 to 8 weeks, molecular methods are important for the determination of drug resistance in ...drug ... See full document