[PDF] Top 20 Rapid Identification of Methicillin Resistant Staphylococcus aureus Using Real Time PCR
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Rapid Identification of Methicillin Resistant Staphylococcus aureus Using Real Time PCR
... to methicillin, but also because it is gener- ally resistant to many other commonly used antimicrobi- ...S. aureus strains was still high in comparison to the findings from other countries ... See full document
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New Real Time PCR Assay for Rapid Detection of Methicillin Resistant Staphylococcus aureus Directly from Specimens Containing a Mixture of Staphylococci
... the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) are generally based on the detection of an ...S. aureus-specific gene target and the mecA ... See full document
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Evaluation of the Xpert Methicillin Resistant Staphylococcus aureus (MRSA) Assay Using the GeneXpert Real Time PCR Platform for Rapid Detection of MRSA from Screening Specimens
... The rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) along with an early implementation of appro- priate intervention has been reported to reduce the prevalence of ... See full document
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Rapid Screening and Identification of Methicillin Resistant Staphylococcus aureus from Clinical Samples by Selective Broth and Real Time PCR Assay
... for methicillin-resistant Staphylococcus aureus (MRSA) by using selective broth and real-time PCR (broth-PCR) was developed and ...by real-time ... See full document
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High Levels of mecA DNA Detected by a Quantitative Real Time PCR Assay Are Associated with Mortality in Patients with Methicillin Resistant Staphylococcus aureus Bacteremia
... Persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia is known to be a poor prog- nostic ...several PCR assays for the detection of MRSA in various clinical samples ... See full document
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A Rapid and High Throughput Screening Approach for Methicillin Resistant Staphylococcus aureus Based on the Combination of Two Different Real Time PCR Assays
... Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that has been responsible for major nosocomial epidemics ...programs, rapid and adequate detection of MRSA is ... See full document
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Detection of Methicillin Resistant Staphylococcus aureus and Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real Time PCR
... a Staphylococcus aureus-specific gene and the mecA gene, responsible for the resistance to methicillin in staphylococci, was ...and real-time PCR conducted with a LightCycler ... See full document
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Evaluation of single and double locus real time PCR assays for methicillin resistant Staphylococcus aureus (MRSA) surveillance
... to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY ... See full document
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Multilocus Sequence Typing of Methicillin Resistant Staphylococcus aureus from an Area of Low Endemicity by Real Time PCR
... System PCR for MLST offers the advantage to perform efficient and rapid amplification, and real-time detec- tion of the seven housekeeping genes in the same PCR ...for ... See full document
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Clinical Application of Real Time PCR to Screening Critically Ill and Emergency Care Surgical Patients for Methicillin Resistant Staphylococcus aureus: a Quantitative Analytical Study
... that PCR can detect MRSA at concentrations too low for reliable detection by the broth-enrichment culture which ...for PCR pro- duces a decrease in the number of organisms subsequently cultured (data not ... See full document
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Rapid Identification of Methicillin ResistantStaphylococcus aureus and Simultaneous Species Confirmation Using Real Time Fluorescence PCR
... ing methicillin-resistant isolates of Staphylococcus epidermidis (n ⫽ 3), Staphylococcus hominis (n ⫽ 2), and Staphylococcus pasteuri (n ⫽ 1) as well as methicillin-susceptible ... See full document
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Identification o f Clinical Methicillin and Mupirocin-resistant Staphylococcus aureus by Multiplex-PCR
... S. aureus by standard biochemical tests for colony morphology, Gram staining, catalase, coagulase, DNase, and novobiocin sensitivity ...by PCR. Screening for methicillin and mupirocin resistance in ... See full document
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Novel Multiplex PCR Assay for Simultaneous Identification of Community Associated Methicillin Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase Negative Staphylococci
... multiplex PCR assay identifying USA300 and USA400 community-associated MRSA strains, detecting PVL and mecA genes, and simultaneously discriminating ...S. aureus from CoNS. The optimized M-PCR was ... See full document
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Comparison of the BD GeneOhm Methicillin Resistant Staphylococcus aureus (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from Intensive Care Unit Patients
... for PCR-positive, cul- ture-negative ...BDG PCR assay can amplify the SCCmec insertion sequence with the respective strains lacking the mecA gene (14, ...with PCR amplification of the organism, or ... See full document
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Novel Determinants of Antibiotic Resistance: Identification of Mutated Loci in Highly Methicillin Resistant Subpopulations of Methicillin Resistant Staphylococcus aureus
... Aliquots (1 l) of the four heteroresistant “parental” cultures of UK13136 (parental strain of family A), ST63/458 (parental strain of family B), E2125 (parental strain of family C), and E4278 (parental strain of family ... See full document
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Rapid Multiplex PCR Assay for Identification of USA300 Community Associated Methicillin Resistant Staphylococcus aureus Isolates
... LNA PCR primer was designed with 6 AT repeats at the 3 ⬘ end and LNA-modified bases near the 5 ⬘ end to strongly drive correct hybridization and PCR amplification when used with an appropriate reverse ... See full document
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High prevalence of Staphylococcus aureus and methicillin resistant S aureuscolonization among healthy children attending public daycare centers in informal settlements in a large urban center in Brazil
... S. aureus and MRSA colonization in comparison to children attending DCCs in non-informal ...S. aureus and MRSA is occurring in DCCs rather than at home, al- though more accurate geocoding will be required ... See full document
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Antimicrobial resistance in atopic dermatitis : need for an urgent rethink
... 6. Fleury OM, McAleer MA, Feuillie C, Formosa-Dague C, Sansevere E, Bennett DE, et al. Clumping Factor B Promotes Adherence of Staphylococcus aureus to Corneocytes in Atopic Dermatitis. Freitag NE, editor. ... See full document
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Methicillin resistant Staphylococcus Aureus (MRSA) in a Malaysian Hospital
... Methicillin resistant Staphylococcus Aureus (MRSA) in a Malaysian Hospital ORIG'INAL ARTICLE Methicillin resistant Staphylococcus Aureus (MRSA) in a Malaysian Hospital I Cheong, FRCP* S C Tan, BSc** Y[.] ... See full document
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... and confirmatory methods (MRSA screen by cefoxitin disc, hVISA/VISA screen by brain heart infusion agar with 6 µg vancomycin and hVISA/VISA confirmation by PAP-AUC method). Agr polymorphism in hVISA/VISA was analysed by ... See full document
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