[PDF] Top 20 Rapid method for testing susceptibility of Mycobacterium tuberculosis by using DNA probes
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Rapid method for testing susceptibility of Mycobacterium tuberculosis by using DNA probes
... M. tuberculosis, which does not enable a homogeneous suspension to be ...new, rapid way of detecting resistance in M. tuberculosis strains which depends on mycobacterial ...for testing the ... See full document
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Rapid detection of Drug Resistant Mycobacterium tuberculosis in Sputum Samples by Direct Nitrate Reductase Assay Method
... treatment, tuberculosis (TB) still accounts for millions cases of active disease and deaths ...common method for detection of drug resistant TB in resource-limited settings is indirect susceptibility ... See full document
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Genotypic Susceptibility Testing of Mycobacterium tuberculosis Isolates for Amikacin and Kanamycin Resistance by Use of a Rapid Sloppy Molecular Beacon Based Assay Identifies More Cases of Low Level Drug Resistance than Phenotypic Lowenstein Jensen Testing
... M. tuberculosis mutations in codons 1401 and 1402 of the rrs gene and mu- tations along the promoter region of the eis ...gene using the primers AMG-F (5=-GCTAGTAATCGCAGATCAGCAACGCTGC-3=) and AMG-R ... See full document
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Rapid, Standardized Method for Determination of Mycobacterium tuberculosis Drug Susceptibility by Use of Mycolic Acid Analysis
... (MDR) Mycobacterium tuberculosis and extrensively drug-resistant (XDR) ...for testing the susceptibility of M. tuberculosis require several days to weeks to ...and susceptibility ... See full document
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Current Perspectives on Drug Susceptibility Testing of Mycobacterium tuberculosis Complex: the Automated Nonradiometric Systems
... excellent method able to provide rapid and reliable results of susceptibility to first- line ...for susceptibility testing in order to limit the expansion of drug-resistant TB, a full ... See full document
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Polymorphisms Associated with Resistance and Cross Resistance to Aminoglycosides and Capreomycin in Mycobacterium tuberculosis Isolates from South Korean Patients with Drug Resistant Tuberculosis
... of Mycobacterium tuberculosis using phenotypic drug susceptibility testing (DST) to determine the extent of resistance to each agent and cross-resistance between ...the DNA ... See full document
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Real Time PCR Using Mycobacteriophage DNA for Rapid Phenotypic Drug Susceptibility Results for Mycobacterium tuberculosis
... a rapid 1- to 3-day phenotypic drug susceptibility test for ...proportion method for the 13 main antituberculous drugs and can permit one to construct an appro- priate MDR or XDR TB regimen ... See full document
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Evaluation of BACTEC Mycobacteria Growth Indicator Tube (MGIT 960) Automated System for Drug Susceptibility Testing ofMycobacterium tuberculosis
... Drug-resistant Mycobacterium tuberculosis strains represent a serious public health ...makes tuberculosis difficult to treat ...the rapid detection of these isolates is critical for the ... See full document
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ϕ2GFP10, a High Intensity Fluorophage, Enables Detection and Rapid Drug Susceptibility Testing of Mycobacterium tuberculosis Directly from Sputum Samples
... Fluorophages are a type of “reporter phage” that inject their DNA specifically into mycobacteria (24). Fluorescence is pro- duced by expression of a fluorescent reporter gene (11) cloned into the phage. Each ... See full document
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Rapid Detection of Mycobacterium tuberculosis Resistance to Second Line Drugs by Use of the Manual Mycobacterium Growth Indicator Tube System
... multidrug-resistant tuberculosis (MDR- TB) caused by Mycobacterium tuberculosis and, recently, exten- sively drug-resistant tuberculosis caused by an MDR strain that is also resistant to any ... See full document
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Multicenter Laboratory Evaluation of the MB/BacT Mycobacterium Detection System and the BACTEC MGIT 960 System in Comparison with the BACTEC 460TB System for Susceptibility Testing of Mycobacterium tuberculosis
... losis susceptibility testing is universally ...drug susceptibility testing procedures, such as the pro- portions methods on Lo ¨wenstein-Jensen or agar medium, are ...alternative method ... See full document
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Direct Drug Susceptibility Testing of Mycobacterium tuberculosis for Rapid Detection of Multidrug Resistance Using the Bactec MGIT 960 System: a Multicenter Study
... drug susceptibility testing of Mycobacterium tuberculosis with liquid medium is well established and of- fers time-saving and reliable ...drug susceptibility testing (DST) can be ... See full document
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Multiplex Real Time PCR Melting Curve Assay To Detect Drug Resistant Mutations of Mycobacterium tuberculosis
... for rapid detection of drug- resistant ...M. tuberculosis. In this assay, dually labeled probes were designed to cover drug resistance-related regions and detect mutations on the basis of the melting ... See full document
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Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis
... A total of 106 isolates of Mycobacterium tuberculosis were tested for drug susceptibility by the conventional 7Hll plate method and by a new rapid radiometric method using special 7H12 l[r] ... See full document
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Rapid Colorimetric Method for Testing Susceptibility of Mycobacterium tuberculosis to Isoniazid and Rifampin in Liquid Cultures
... of tuberculosis (TB) worldwide has been ac- companied by an increase in the incidence of drug-resistant TB and, more importantly, also in that of multidrug-resistant (MDR) TB (strains resistant to at least ... See full document
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Rapid susceptibility testing of Mycobacterium tuberculosis (H37Ra) by flow cytometry
... is rapid because conventional susceptibility tests depend on mul- tiplication of the microorganism for the detection of visible growth, which requires several days (12, 16, ...our method, flow ... See full document
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Detection of a Point Mutation Associated with High Level Isoniazid Resistance in Mycobacterium tuberculosis by Using Real Time PCR Technology with 3′ Minor Groove Binder DNA Probes
... Mycobacterium tuberculosis. One of the mainstay drugs for the treatment of tuberculosis is isonia- zid ...M. tuberculosis, initially reported in 1952 (4, 18), is hampered due to the presence ... See full document
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PCR Restriction Fragment Length Polymorphism for Rapid, Low Cost Identification of Isoniazid Resistant Mycobacterium tuberculosis
... Tuberculosis (TB) control is threatened by increasing resis- tance to first-line antituberculous agents. The WHO estimates that in the year 2004, 4.3% of new TB cases globally were multidrug-resistant TB (MDR-TB) ... See full document
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Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures
... In the Danish study, results obtained with 30 AFB⫹ BACTEC 12B cultures were compared with the results ob- tained by tests with AccuProbe (Table 3). Sixteen samples were identified as MTC and 10 samples were identified as ... See full document
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Rapid susceptibility testing for nontuberculosis mycobacteria using flow cytometry
... potential of M. tuberculosis (4, 13, 23). The delay in the accep- tance of the pathogenic roles of these organisms arises from their relatively low level of intrinsic virulence, their propensity to commensally ... See full document
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