[PDF] Top 20 A recombinant antigen based enzyme linked immunosorbent assay (ELISA) for lungworm detection in seals

... elephant seals. These im- mature lungworm stages were mostly found in the right ventricle of the heart as opposed to the lung, as ob- served in harbour and grey ...elephant seals and therefore does ... See full document

... a recombinant Bay- lisascaris antigen, BpRAG1, and its utility in the diagnosis of Baylisascaris larva migrans in human ...BpRAG1 antigen showed a sensitivity of 85%, with 17 of 20 samples reacting ... See full document

... hydrogen peroxide was added to each well. The reaction was terminated after 30 min by adding 100 ␮l of 2 N sulfuric acid to each well. The absorbance at 450 nm was then monitored. SpaA416 ELISA. The SpaA416 ELISA was ... See full document

... the recombinant protein were solubilized with Sarkosyl, and the protein was enriched from the crude bacterial extract by thiol-affinity ...The recombinant protein was used to develop a rapid, sensitive, and ... See full document

... the detection of a greater number of IgG-positive serum sam- ples in the ACG-ELISA contributed to the presence of cross- reactive nonstructural proteins in the SMB ... See full document

... of recombinant rE2-CHIKV for coating ELISA plate wells was determined based on a clear detection of anti-CHIKV antibodies using mouse hyperimmune serum, which was 10 times higher than the cut ... See full document

... the antigen capture ELISA based on the mean OD value ⫹ 3 SDs of 79 sera and 5 liver specimens from EBO-R-uninfected cynomolgus ma- ...the antigen capture ELISA using either Res2-6C8 or Res2-1D8 for ... See full document

... Cross-reactivity of turkey sera with IBV assessed using a commercial IBV whole-virus ELISA. The cross-reactivity of turkey sera against IBV antigens was assessed using commercially available IBV whole-virus-coated ELISA ... See full document

... We identified four HGE patients whose sera did not have detectable antibodies in an ELISA with the HGE-44–MBP antigen, but specific antibodies to 44-kDa proteins were de- tected by whole-cell immunoblot analysis. ... See full document

... the assay should be able to recognize the highly immunogenic, natively folded RV GP present on the virus particle and should circumvent problems associated with the estimation of the poorly immu- nogenic soluble ... See full document

... virus antigen-detection enzyme-linked immunosorbent assay (ELISA) system using a novel monoclonal antibody (MAb) to the nucleoprotein ...purified recombinant NP (rNP) was ... See full document

... RAP-1 antigen for ELISA, we constructed a recombinant baculovirus capable of expressing the ...with recombinant baculovirus ...the recombinant protein was larger than that of the native RAP-1 ... See full document

... The detection of the viral antigen can be an alternative to isolation of the virus or the detection of viral nucleic acids, IgG, or ...viral antigen detection assay for the ... See full document

... The remaining conditions of the test were: (i) purified antigen concentrations ranging from 1 to 1,000 ng/ml; (ii) a 1:500 dilution of rabbit anti-SSS-III serum, based on a titration in [r] ... See full document

... reference antigen test, and the DUO Ultra) were repeated in duplicate following a test algorithm ...blot assay (Novapath HIV-1 Immunoblot; Bio-Rad, Hercules, ...(RT-PCR) assay (Amplicor HIV-1 Monitor ... See full document

... Increased iELISA titers against O157, compared to prevac- cination titers, were present in B. abortus-immune cattle, and antibodies cross-reactive with O antigen were detected by West- ern blot that had detected ... See full document

... In this study, although the positive results (33.3%) in the group of patients with clinically diagnosed typhoid fever was considered false-positive, this might be a true positive result [r] ... See full document

... The mean background reaction produced by six uninfected cell controls inoculated with normal nasal wash specimens is indicated by the bar. The dashed line (positive cutoff) represents 3 [r] ... See full document

... (36) found it necessary to per- form a confirmatory test (blocking assay) on all sera positive for hepatitis B surface antigen by the routine ELISA. Our experience with sera agrees with [r] ... See full document

... After another hour of incubation of 37°C and four washes in rinsing solution, 100 ,ul of the affinity- purified rabbit RVF antibodies diluted 1:640 in ELISA buffer (containing 1% normal [r] ... See full document