[PDF] Top 20 Replication and gene expression of hepatitis B virus in a transgenic mouse that contains the complete viral genome.

... Indeed, in human chronic carriers containing integrated complete copies of HBV DNA without replication, only HBsAg is produced 2, suggesting that the core or pregenome promoter does not [r] ... See full document

... the gene expression profile in the livers of HBV transgenic mice before and after the adoptive transfer of HBV-specific CTLs or infection with LCMV, which inhibit HBV replication by IFN-␥- and ... See full document

... laboratory mouse is genetically and immunologically defined, and a large collection of genetically modified animals ...with replication competent HBV genomes have been established and represented to be ... See full document

... pWTD contains a head-to-tail dimer of ...pWTH contains one copy of the wild-type HBV sequence but with a HindIII site engineered at nt ...k B double-stranded oligonucleotide from the immunoglobulin ... See full document

... HBV-transgenic mouse hepatocyte cell culture system that replicates HBV and is sensitive to the antiviral activities of cytokines, reflecting the behavior of the virus and the cytokines in vivo (3, ... See full document

... in transgenic-mouse serum was determined by two different quantitative dot blot procedures in our ...filtered transgenic-mouse serum pooled from lineages ...filtered ... See full document

... Hepatitis B virus X (HBX) is essential for the productive infection of hepatitis B virus (HBV) in vivo and has a pleiotropic effect on host ...enhanced viral ... See full document

... HBV gene expression and replication can be inhibited by hepatocyto- tropic DNA viruses, like HBV, providing additional support to the notion that similar antiviral events can limit HBV infection ... See full document

... of viral RNA (39). Finally, the double-transgenic phenotype could theoretically be repro- duced if the abundant precore RNA can compete with the ...that replication efficiency may also be af- fected ... See full document

... HBsAg expression is crucial for the pathogenesis of HBV, as the envelope proteins is essential for the viral particle formation, infectivity, and immune response (2, ...HBsAg expression was observed ... See full document

... of transgenic mice. The construct used for generation of cex-transgenic mice (20) comprised a fragment of the HBV genome containing the core ORF (HBc/e), the core promoter (Pc/e), and the enhancer ... See full document

... dominant virus population of mothers M1 and M4 changed back to the wild type in the infants (Figure ...in B- T-cell epitopes play a significant role in ...change viral pathogenicity due to an altered ... See full document

... both gene products is ...HBeAg-matched transgenic mice (four mice per group) that were either heterozygous or homozygous for the indicated null mutation were injected intravenously with a single dose of ... See full document

... HBV genome or a mutated HBV genome that expresses all viral proteins except the X ...the transgenic mice is illustrated in ...HBV genome and has been used to generate transgenic ... See full document

... DNA virus in the Hepad- naviridae family. The HBV genome includes four genes: pol, env, precore, and X, respectively, which encode the viral DNA polymerase, envelope protein, precore protein (which ... See full document

... core gene (HBV nt 2125 to ...core gene (HBV nt 2125 to 2145) was conserved in different subtypes of the HBV genome ...reporter expression was not influenced by alteration of HBV-miR-3 levels ... See full document

... plasmid contains in-frame sequences encoding the KRAB repressor immediately upstream of a TALE tar- geting the rhodopsin gene, together with hemagglutinin (HA) and nuclear localization signal ...HBV ... See full document

... chronic hepatitis B, persis- tent viral infection and ineffective T cell responses lead to chronic inflammatory liver damage and continuous compensatory proliferation of hepatocytes, which may lead to ... See full document

... coronavirus mouse hepatitis virus (MHV) contains a large open reading frame embedded entirely within the 5 * half of its nucleocapsid (N) ...internal gene (designated I) is in the 1 1 ... See full document

... DNMT expression leads to methylation of host CpG ...for viral particles (dotted ...mRNA expression in HBV-exposed cells was delayed compared to that in control cells, with significant differences at ... See full document