[PDF] Top 20 Replication of a hepatitis C virus replicon clone in mouse cells
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Replication of a hepatitis C virus replicon clone in mouse cells
... Hepatocyte) cells were obtained from Marco Tripodi (Università La Sapienza, ...immortalized mouse hepatocyte cell line was derived from the liver of transgenic mice, which express the constitutively active ... See full document
9
Changes in Rhinovirus Protein 2C Allow Efficient Replication in Mouse Cells
... HeLa cells to produce high-titer virus ...ICAM-L cells, only 16/DN and 16/MVDN pro- duced high titers ...ICAM-L cells. The results of one-step growth analysis in ICAM-L cells show that ... See full document
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Nonhepatic Cell Lines HeLa and 293 Support Efficient Replication of the Hepatitis C Virus Genotype 2a Subgenomic Replicon
... full-length replicon system using the JFH-1 clone and various cell lines is thus ...these cells can support HCV replication but will not express the receptors necessary for viral ... See full document
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Ribavirin Resistance in Hepatitis C Virus Replicon-Containing Cell Lines Conferred by Changes in the Cell Line or Mutations in the Replicon RNA
... the replicon is ...the C terminus of NS5A contains a variable region termed V3, which includes Gly404, and mu- tations in this region may affect response to antiviral treatment ...NS5A C-terminal ... See full document
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The Hepatitis C Virus RNA-Dependent RNA Polymerase Membrane Insertion Sequence Is a Transmembrane Segment
... the C terminus of NS5B is translo- cated to the ER lumen also in the context of a functional HCV replication complex, the NS5B-gt construct was introduced into a subgenomic ...This replicon construct ... See full document
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Characterization of Hepatitis C Virus Subgenomic Replicon Resistance to Cyclosporine In Vitro
... simplex virus, and vaccinia virus (8, ...HIV replication has been the subject of intensive studies that have led to the realization that CypA in target cells can regulate viral ... See full document
12
Apoptosis induced in mouse hepatitis virus-infected cells by a virus-specific CD8+ cytotoxic T-lymphocyte clone.
... 7540-7545 0022-538X/94/$04.00+0 Copyright C 1994, American Society for Microbiology Apoptosis Induced in Mouse Hepatitis Virus-Infected Cells by a Virus-Specific CD8+ Cytotoxic T-Lymphoc[r] ... See full document
6
The α Isoform of Protein Kinase CKI Is Responsible for Hepatitis C Virus NS5A Hyperphosphorylation
... HCV replication upon silencing of ...HCV replication is inhibited as a consequence of reduced expression of the CKI- ␣ isoform by RNA interference ...Huh7 cells which stably express an HCV subgenomic ... See full document
8
Mathematical Modeling of Subgenomic Hepatitis C Virus Replication in Huh-7 Cells
... replicons (35, 50) or in liver cells (9, 27) is still unknown. It might be that host factors, required for synthesizing minus- strand RNA, are responsible for this asymmetry or that there are different rate ... See full document
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Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
... of cells containing replicons with R393A, F438A, and W501A muta- tions to form colonies indicates that a helicase which can hy- drolyze ATP, bind nucleic acids, and unwind DNA is not suf- ficient to support HCV ... See full document
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Interferons and Ribavirin Effectively Inhibit Norwalk Virus Replication in Replicon-Bearing Cells
... ribavirin-treated cells moderately reversed the antiviral effect of ribavirin ...HG23 cells treated with ribavirin (100 M) or without ribavirin for 48 h, revealed that there was no mutation in the RT-PCR ... See full document
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Amino Acid Mutations in the NS4A Region of Hepatitis C Virus Contribute to Viral Replication and Infectious Virus Production
... J6CF replication. (A) Replication capacity of J6/5BSLX-JFH1 subgenomic replicons with ...Huh-7.5.1 cells, and the cells were harvested at 4, 24, and 48 h ...RNA-transfected cells. In ... See full document
16
In vitro replication of mouse hepatitis virus strain A59.
... Before attempting an in vitro assay for coronavirus RNA synthesis, the kinetics of MHV-A59 polymerase activity in infected Sac- cells were determined.. To synchronize expression of the v[r] ... See full document
7
Manipulation of both virus- and cell-specific factors is required for robust transient replication of a hepatitis C virus genotype 3a sub-genomic replicon
... con-harbouring cells [16], we sought to reproduce this ...Huh7.5 cells and selected with G418 for three weeks, after which time a small number of colonies of stable SGR-harbouring cells were ...These ... See full document
12
Hepatitis C Virus RNA Synthesis in a Cell-Free System Isolated from Replicon-Containing Hepatoma Cells
... RNA replication can be analyzed is cell based and utilizes autonomously replicating HCV-derived RNAs ...These replicon RNAs have the au- thentic HCV 5⬘- and ...encephalomyocarditis virus (EMCV) IRES ... See full document
9
Effect of Alpha Interferon on the Hepatitis C Virus Replicon
... viral replication in cells is incomplete, it could be used as one predictor for the ability of a particular construct to replicate in Huh7 cells ... See full document
8
In Vitro RNA Replication Directed by Replicase Complexes Isolated from the Subgenomic Replicon Cells of Hepatitis C Virus
... 3 ⴕ dNTPs as inhibitors of HCV RC. Establishment of the HCV replicase assay will help to identify and validate potential inhibitors targeting the RNA replication process. We first tested whether 3⬘ ... See full document
6
Effect of Hepatitis C Virus (HCV) NS5B-Nucleolin Interaction on HCV Replication with HCV Subgenomic Replicon
... wild-type replicon was represented by MA, in which S232 of NS5A was altered to I, because this mutant replicon can efficiently replicate in Huh7 cells (36, ...the replicon MA/cm211, each of ... See full document
9
A Respiratory Syncytial Virus Replicon That Is Noncytotoxic and Capable of Long-Term Foreign Gene Expression
... con-containing cells with plasmids MP340 and MP341, which contain codon- optimized versions of the RSV strain D53 (A strain) F gene and the A2 G gene, ...the cells from the tissue culture dishes into the ... See full document
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Bile Acids Promote the Expression of Hepatitis C Virus in Replicon-Harboring Cells
... understanding virus replication and developing improved therapeutics have been severely ...HCV replicon-harboring cells has provided a valuable tool for studying the basic biology of the ... See full document
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