[PDF] Top 20 Requirement for Cellular Cyclin-Dependent Kinases in Herpes Simplex Virus Replication and Transcription

... HSV replication requires a cellular cdk(s), the question arises as to which of the many cdks are ...HSV replication in the experiments presented ...than cellular genes ...for ... See full document

... Although herpes simplex virus (HSV) replicates in noncycling as well as cycling cells, including terminally differentiated neurons, it has recently been shown that viral replication requires ... See full document

... for cellular cyclin-dependent kinases (cdks), Rosco- vitine (Rosco) and Olomoucine (Olo), block the replication of herpes simplex virus ...HSV replication ... See full document

... Pharmacological cyclin-dependent kinase (cdk) inhibitors (PCIs) block replication of several viruses, in- cluding herpes simplex virus type 1 (HSV-1) and human immunodeficiency ... See full document

... human herpes simplex virus type 1 (HSV-1) regulatory protein, VP16, stimulates productive infection by activating transcription of viral immediate-early (IE) ...vates transcription from ... See full document

... IE transcription factors (BZLF1 and BRLF1 proteins) transactivate viral promoters, triggering the EBV lytic ...DNA replication and metabolism (10) and are assembled into replication compart- ments ... See full document

... HSV-1 replication by regulating all stages of mRNA biogenesis, including transcription, RNA processing, and export for translation ...mutant virus at 2 h postinfection, suggested that p53 played a ... See full document

... SAPK-induced transcription factors, ATF-2, c-Jun, and AP-1, during HSV infection has been reported (56, 97), the impact of these activations on cellular and viral gene expression has not been fully ...viral ... See full document

... the virus life cycle appears to be critical for viral ...the cyclin-dependent kinase subunits are relatively ...the cyclin component, viruses overcome a rate-limiting step in cdk ...for ... See full document

... DNA replication assays, raising concern that there might have been competition between the test plasmids and the internal standard for viral and cellular ... See full document

... is dependent on viral DNA replication, and the stimulatory effect of VP16 is ...The requirement for VP16 in phase II argues that chromatin remodeling is critical for full ...of cellular H3K27 ... See full document

... the cyclin binding site, and Ink4 CKIs have been shown to block the formation of Cdk4/cyclin D complexes (Coleman et aL, ...is dependent on the status of Rb (Guan et aL, ... See full document

... the replication pattern of HSV differed between MEFs and BMDCs because of differences in adherence to plastic substrates in ...viral replication in the IRF-3 ⫺/⫺ BMM resembled that seen in BMDCs, with ... See full document

... MILLETTE* Department of Biology, Portland State University, and Department of Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97207 Received 18 February [r] ... See full document

... Addition of fraction VIII to the in vitro assay reduced transcription from the immediate-early gene fragment and stimulated transcription from DNA fragments cut from early and late genes[r] ... See full document

... Here, we have shown for the first time that WDR11 is a TGN- resident protein and that its redistribution is a result of the virus- induced fragmentation of the Golgi apparatus and TGN (8, 15, 51–54). Previous ... See full document

... Generation of albumin enhancer/promoter-regulated re- combinant HSV-1 (G92A). We constructed an HSV-1 recom- binant regulated by the albumin enhancer/promoter and termed G92A (Fig. 1A). G92A is derived from d120, an ... See full document

... medium. Replication experiments were performed as described by Stow and McMonagle (49) and Stow and Davison ...Total cellular DNA was prepared 48 h after superinfection by the addition of 5 ml of lysis ... See full document

... HSV replication complex to the AAV ...was dependent on the AAV origins of DNA replication ...HSV-1 replication pro- teins requires the AAV ITRs. To test if Rep68-dependent DNA synthesis ... See full document

... (Fig. 3A) is comparable to the 13.1- ␮ m average seen for PRV in vivo using neuronal cell cultures (58). The average velocity (0.58 ␮ m/s) of movement in our chamber system (Fig. 3B) is also in good agreement with the ... See full document