[PDF] Top 20 Role of Electrostatics in the Assembly Pathway of a Single-Stranded RNA Virus
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Role of Electrostatics in the Assembly Pathway of a Single-Stranded RNA Virus
... the RNA within each protocap- sid in order to drive nucleocapsid ...the RNA, distinct from the overcharged state found for the final assembly ...efficient assembly under the in vitro ... See full document
8
Molecular details of the double-stranded RNA virus replication and assembly
... in virus assembly mainly due to differences in virion structure and complexity of these ...a single capsid protein and two dsRNA genome segments and the RdRp is located inside the ...the ... See full document
67
Characterization of a Novel Megabirnavirus from Sclerotinia sclerotiorum Reveals Horizontal Gene Transfer from Single-Stranded RNA Virus to Double-Stranded RNA Virus
... Based on sequence analysis and biological data presented in this study, we propose that SsMBV1 might be a novel dsRNA megabirnavirus, although a definitive definition of L2-dsRNA/ SsMBV1 (L2-dsRNA) as an authentic genome ... See full document
13
In situ structures of the genome and genome-delivery apparatus in a single-stranded RNA virus.
... capsid assembly as previously proposed 15-17,21 , the higher resolution of the above 16 stem-loops indicates stronger interactions with capsid proteins, and thus perhaps a more important role in the capsid ... See full document
25
Investigation of the role of GBF1 in the replication of positive-sense single-stranded RNA viruses
... of GBF1 as a cellular factor required for hepatitis C virus RNA replication. Carpp LN, Rogers RS, Moritz RL, Aitchison JD[r] ... See full document
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Packaging signals in single-stranded RNA viruses: nature’s alternative to a purely electrostatic assembly mechanism.
... to assembly, is the apparent lack of specificity for RNA in in vitro assays of spontaneous co-assembly to form virus-like ...that assembly is driven largely by electrostatics ... See full document
11
Localization of the Single-Stranded RNA-Binding Domains of Bluetongue Virus Nonstructural Protein NS2
... All intact and truncated NS2 proteins used for this project were produced by a prokaryotic expression system. Since BTV infects mammalian cells, experiments are in progress to exam- ine how and where intact and truncated ... See full document
8
The hepatitis C virus (HCV) is a single-stranded RNA virus of the Hepacivirus genus in the Flaviviridae family (Figure 2).
... If a person is found to have a positive hepatitis C PCR test (also known as "active viremia"), a test for genotype is indicated. While there is no known correlation between either viral load or genotype and disease ... See full document
8
Characterization of a Novel Megabirnavirus from \u3cem\u3eSclerotinia sclerotiorum\u3c/em\u3e Reveals Horizontal Gene Transfer from Single-Stranded RNA Virus to Double-Stranded RNA Virus
... Based on sequence analysis and biological data presented in this study, we propose that SsMBV1 might be a novel dsRNA megabirnavirus, although a definitive definition of L2-dsRNA/ SsMBV1 (L2-dsRNA) as an authentic genome ... See full document
15
Disruption of Specific RNA-RNA Interactions in a Double-Stranded RNA Virus Inhibits Genome Packaging and Virus Infectivity.
... Bluetongue virus (BTV) causes hemorrhagic disease in economically important ...double-stranded RNA molecules (S1-S10) which have been suggested to follow a sequential packaging pathway from ... See full document
23
A Role for Single-Stranded Templates in Cell-Free Adeno-Associated Virus DNA Replication
... free single strands. Abundant single-stranded DNA is not detected in the early stages of in vivo AAV DNA replication, when the amounts of duplex forms are increasing greatly ...if single ... See full document
11
Double-stranded RNA Bacteriophage phi6 : Self-assembly and Maturation of the Procapsid
... The role of P4 in PC assembly and maturation ...PC assembly, as is the case for phage P22 assembly (see ...a single P4 hexamer was not sufficient to induce formation of the complete ... See full document
51
Reovirus Protein ςNS Binds in Multiple Copies to Single-Stranded RNA and Shares Properties with Single-Stranded DNA Binding Proteins
... animal virus proteins and NS but cannot destabilize DNA duplexes ...a role in genome replication similar to that played by the ssDNA binding ...reovirus RNA polymerase and other potential cofactors ... See full document
10
The RNA-Binding Protein of a Double-Stranded RNA Virus Acts like a Scaffold Protein
... disease virus (IBDV), a nonenveloped, double-stranded RNA (dsRNA) virus with a T ⫽13 icosahedral capsid, has a virion assembly strategy that initiates with a precursor particle based on ... See full document
14
In Vivo SELEX of Single-Stranded Domains in the HIV-1 Leader RNA
... immunodeficiency virus type 1 (HIV-1) RNA genome is a strongly conserved sequence that encodes several regulatory motifs important for viral ...short single-stranded ...the ... See full document
11
The Double-Stranded RNA-Binding Protein Staufen Is Incorporated in Human Immunodeficiency Virus Type 1: Evidence for a Role in Genomic RNA Encapsidation
... viral RNA is targeted to sites of assem- bly. Presumably, HIV-1 RNA-protein associations with the precursor p55 Gag could come into play to influence genomic RNA localization to sites of ...genomic ... See full document
11
Evidence that the genome of hepatitis A virus consists of single-stranded RNA.
... Nucleic acid was extracted from purified hepatitis A virus, radiolabeled with.. "2I, and shown to consist of single-stranded RNA which sediments at 35S and contains sequences of poly[r] ... See full document
5
Poliovirus single-stranded RNA and double-stranded RNA: differential infectivity in enucleate cells.
... Two types of control nucleate cells were prepared for these experiments: (i) normal cells never treated with cytochalasin B and (ii) cells exposed to 10 ,ug of cytochalasin B per ml in a[r] ... See full document
5
Newcastle Disease Virus-Specific RNA: Polyacrylamide Gel Analysis of Single-Stranded RNA and Hybrid Duplexes
... The applicability of polyacrylamide gel electrophoresis of hybrid duplexes to the analysis of viral genome structure is discussed.. A large part of virus-specific RNA formed in.[r] ... See full document
8
In vitro phosphorylation and purification of a nonstructural protein of bluetongue virus with affinity for single-stranded RNA.
... Quantities (5 ,ug) of BTV mRNA (A), cellular ssRNA (B), and BTV dsRNA (C) were mixed with different amounts of P100 SW fractions either from BTV- infected cells (A) or from uninfected ce[r] ... See full document
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