[PDF] Top 20 A Role for Single-Stranded Templates in Cell-Free Adeno-Associated Virus DNA Replication
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A Role for Single-Stranded Templates in Cell-Free Adeno-Associated Virus DNA Replication
... Some single-stranded replication templates are produced by a mechanism other than strand displacement ...some single- stranded molecules serving as replication ... See full document
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Rep-Dependent Initiation of Adeno-Associated Virus Type 2 DNA Replication by a Herpes Simplex Virus Type 1 Replication Complex in a Reconstituted System
... full-length DNA chains that are essentially free of nicks ...of replication products migrates in the first di- mension at a position consistent with their being mAAV ...to free ... See full document
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Role of the Herpes Simplex Virus Helicase-Primase Complex during Adeno-Associated Virus DNA Replication
... carrying single amino acid substitutions in either helicase motif I or motif IV were shown to be negative for HSV DNA replication ...AAV replication in hr99-infected ...AAV DNA ... See full document
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Complete In Vitro Reconstitution of Adeno-Associated Virus DNA Replication Requires the Minichromosome Maintenance Complex Proteins
... AAV DNA replication may require the cellular single-stranded DNA bind- ing protein replication factor A (RPA) (18), and supplemen- tation of uninfected extracts with Ad DBP ... See full document
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Cellular Proteins Required for Adeno-Associated Virus DNA Replication in the Absence of Adenovirus Coinfection
... vitro adeno-associated virus (AAV) DNA replication ...crude adeno- virus (Ad)-infected HeLa cell cytoplasmic extract to catalyze origin of ... See full document
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Adeno-Associated Virus Replication Induces a DNA Damage Response Coordinated by DNA-Dependent Protein Kinase
... parvovirus adeno-associated virus (AAV) contains a small single-stranded DNA genome with inverted terminal repeats that form hairpin ...cellular replication machinery ... See full document
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Involvement of Cellular Double-Stranded DNA Break Binding Proteins in Processing of the Recombinant Adeno-Associated Virus Genome
... essential role in chromosome telomere length maintenance by preventing end-to-end fusion of ...telomeric DNA and that this function is conserved from yeasts to humans (9, 24, ...the cell would treat ... See full document
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In Vitro Packaging of Adeno-Associated Virus DNA
... Adeno-associated virus (AAV) is a parvovirus and is com- posed of three structural proteins and a linear, single-stranded DNA (ssDNA) genome of approximately ...for virus ... See full document
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Intracellular Viral Processing, Not Single-Stranded DNA Accumulation, Is Crucial for Recombinant Adeno-Associated Virus Transduction
... second-strand DNA synthesis is a rate-limiting step for rAAV transduction, which was based on the existence of abundant amounts of ss DNA after AAV infection and few double-stranded ...these ... See full document
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cis Effects in Adeno-Associated Virus Type 2 Replication
... The DNA species rep- resented by these bands are too large to have been encapsi- ...Viral DNA present in 293T cells transfected with pDG and ...AAV DNA; procedure 2 without NaOH (lane 5), ... See full document
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In vitro replication of adeno-associated virus DNA: enhancement by extracts from adenovirus-infected HeLa cells.
... the replication of adeno-associated virus type 2 (AAV2) ...the replication of linear duplex AAV ...examine replication of linear duplex AAV DNA in extracts from either ... See full document
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Regulation of Adeno-Associated Virus DNA Replication by the Cellular TAF-I/Set Complex
... chromatinized templates in vitro ...promoting replication and transcription of the adenovirus genome when this was compacted with basic viral proteins into a structure called the “core” (26, ...template ... See full document
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Purification of Host Cell Enzymes Involved in Adeno-Associated Virus DNA Replication
... Adeno-associated virus (AAV) replicates its DNA by a modified rolling-circle mechanism that exclusively uses leading strand displacement ...AAV DNA replication, we fractionated ... See full document
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Role of the Adenovirus DNA-Binding Protein in In Vitro Adeno-Associated Virus DNA Replication
... Ad DNA replication, the DNA polymerase and the terminal pro- tein make no contribution to AAV DNA ...Ad DNA-binding protein (Ad-DBP) have been ambig- uous (reviewed in reference ...AAV ... See full document
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Nucleoside Analogue Mutagenesis of a Single-Stranded DNA Virus: Evolution and Resistance
... into DNA may offer an ...mosaic virus (28) and 36% of the uracil of poliovirus (44) can be replaced by 5-FU, and although we did not determine this percentage in X174, it is probably ...the virus ... See full document
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Genetic analysis of adeno-associated virus: properties of deletion mutants constructed in vitro and evidence for an adeno-associated virus replication function.
... 611 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Transfection of a pBR322-based, recombinant plasmid, pAV2, containing the entire adeno-associated virus AAV type 2 g[r] ... See full document
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Studies on the Mechanism of Replication of Adenovirus DNA II. The Nature of Single-Stranded DNA in Replicative Intermediates
... Hybridization experiments of the singlestranded DNA with isolated complementary strands of adenovirus type 5 DNA showed that this DNA hybridized only with the viral L-strand the strand w[r] ... See full document
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The adeno-associated virus rep gene inhibits replication of an adeno-associated virus/simian virus 40 hybrid genome in cos-7 cells.
... Because the rep proteins is required for utilization of the TRs as origins of replication in helper virus-infected cells, it seems logical that the cis-active target sequences required f[r] ... See full document
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Replication of adeno-associated virus in synchronized cells without the addition of a helper virus.
... In the present contribution, we show that in cell populations treated with hydroxyurea by using conditions under which the majority of cells are arrested in the S phase prior to infectio[r] ... See full document
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Role of Electrostatics in the Assembly Pathway of a Single-Stranded RNA Virus
... in excess of what is found in the final capsid structure. More pre- cisely, complete packaging of all of the RNA in solution, irrespec- tive of its length (from 140 nucleotides [nt] to 12,000 nt), called for the total ... See full document
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