[PDF] Top 20 Rotavirus RNA Replication Requires a Single-Stranded 3′ End for Efficient Minus-Strand Synthesis
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Rotavirus RNA Replication Requires a Single-Stranded 3′ End for Efficient Minus-Strand Synthesis
... G8d45-543 RNA contains the 59 stem-loop of the wild-type RNA but that this structure is not positioned opposite the 39 UTR of the RNA and therefore that G8d45- 543 does not contain a 59-to-39 ... See full document
10
Recognition of the Core RNA Promoter for Minus-Strand RNA Synthesis by the Replicases of Brome Mosaic Virus andCucumber Mosaic Virus
... The 3⬘ ends of BMV plus-strand RNAs have well-conserved sequences and structural features wherein the terminal 135 nucleotides (nt) can form tRNA-like structures (4, 18, ...of minus-strand ... See full document
9
Interaction between the Cellular Protein eEF1A and the 3′-Terminal Stem-Loop of West Nile Virus Genomic RNA Facilitates Viral Minus-Strand RNA Synthesis
... cells. 3 ⬘ ( ⫹ ) SL RNA probes con- taining mutant C or D substitutions showed a 40% or 37% decrease in relative binding activity to eEF1A, respectively, compared to that of the parental SL ...a ... See full document
16
Poly(A) at the 3′ End of Positive-Strand RNA and VPg-Linked Poly(U) at the 5′ End of Negative-Strand RNA Are Reciprocal Templates during Replication of Poliovirus RNA
... positive-strand RNA viruses ...the 3 ⬘ termini of their RNA genomes (16, 25), yet the mechanisms by which 3 ⬘ poly(A) sequences are derived during viral replication are un- ... See full document
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Rotavirus VP1 alone specifically binds to the 3' end of viral mRNA, but the interaction is not sufficient to initiate minus-strand synthesis.
... the RNA-protein complexes does not require the participation of other viral ...by RNA polymerases (6, 7, 18), and is a common minor component of complexes with replicase activity ...the 3 9 ... See full document
8
Specific hepatitis B virus minus-strand DNA synthesis requires only the 5' encapsidation signal and the 3'-proximal direct repeat DR1.
... proper minus-strand DNA pro- ...one end of the pregenome to the other remains to be ...the 3 9 -proximal polyad- enylation signal has been explained by the absence of upstream activating ... See full document
5
In Vitro Synthesis of Minus-Strand RNA by an Isolated Cereal Yellow Dwarf Virus RNA-Dependent RNA Polymerase Requires VPg and a Stem-Loop Structure at the 3′ End of the Virus RNA
... (CYDV) RNA has a 5 ⴕ -terminal genome-linked protein ...template-dependent RNA-dependent RNA polymerase (RdRp) has been produced from a CYDV membrane-bound RNA polymerase by treatment with BAL ... See full document
9
Mechanism of Stimulation of Plus-Strand Synthesis by an RNA Replication Enhancer in a Tombusvirus
... predicted single-stranded re- gion between SL1-III( ⫺ ) and SL2-III( ⫺ ) hairpins in RIII( ⫺ ), was susceptible to cleavage by double-strand-specific V1 nu- clease (27), a finding that is consistent ... See full document
9
Rotavirus RNA polymerase requires the core shell protein to synthesize the double-stranded RNA genome.
... for minus-strand RNA synthesis ...[g8R39d(-9)-(-67)], synthesis of only trace levels of dsRNA was ...cis-acting replication signals in gene 8 mRNA that were identified before ... See full document
9
Role of the 3′ tRNA-Like Structure in Tobacco Mosaic Virus Minus-Strand RNA Synthesis by the Viral RNA-Dependent RNA Polymerase In Vitro
... TYMV 3⬘ tRNA-like region for minus-strand synthesis in vitro was the ACCA end (10, 39, ...ACCA 3⬘ end, although increased template length with proper base stacking ... See full document
10
Secondary Structure of the 3′ Terminus of Hepatitis C Virus Minus-Strand RNA
... vitro replication studies performed with recombinant NS5B showed that the minimal RNA frag- ment required for efficient replication of the (⫺) strand spans nt ⫺239 to ⫺1 (43), which ... See full document
11
Poliovirus Requires a Precise 5′ End for Efficient Positive-Strand RNA Synthesis
... poliovirus replication (17, 36), and several possible re- pair mechanisms could be envisioned: (i) the 5⬘ end of the input RNA has to be trimmed by a 5⬘-exonucleolytic activity to the correct length ... See full document
7
Requirements at the 3′ End of the Sindbis Virus Genome for Efficient Synthesis of Minus-Strand RNA
... restore RNA synthesis is becoming a com- mon ...⬘ end of the genome allowed mutant nsP4 to function in RNA replication ...the 3 ⬘ sequence of the minus strand ... See full document
10
Identification of cis-Acting Nucleotides and a Structural Feature in West Nile Virus 3′-Terminus RNA That Facilitate Viral Minus Strand RNA Synthesis
... flavivirus 3= SL RNA that may be involved in regulating minus strand RNA ...intact 3= SL may occasionally occur after a ribosome terminates translation of the genomic ...the ... See full document
15
Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers.
... BMV RNA 3 were changed from the wild-type sequence of 5 9 -ACCA-3 9 to 5 9 -GCCA-3 9 ...stimulated minus-strand RNA synthesis to the same level that GpG did, would ... See full document
5
A Pseudoknot in a Preactive Form of a Viral RNA Is Part of a Structural Switch Activating Minus-Strand Synthesis
... of RNA to switch conformations in response to different physiological conditions has fundamental implications for the regulation of many cellular processes, in- cluding transcription termination, protein ... See full document
11
Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase.
... 769 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage 46 transcribed large, medium, and s[r] ... See full document
5
Mechanism of Replication of Single-Stranded φX174 DNA VII. Circularization of the Progeny Viral Strand
... This experiment shows that linear strands isolated from particles produced in the ligase-deficient host are intrinsically capable of becoming covalently closed circular SS DNA, but only [r] ... See full document
11
Requirements for Assembly of Poliovirus Replication Complexes and Negative-Strand RNA Synthesis
... (PV) RNA replication have contributed to new insights into this complex ...viral RNA (vRNA) in various steps leading to production of new vRNA ...template-dependent RNA synthesis and is ... See full document
10
Sindbis virus RNA-negative mutants that fail to convert from minus-strand to plus-strand synthesis: role of the nsP2 protein.
... the end of the chase period, the cells were scraped into ice-cold PBS buffer without calcium and magnesium ions with a cell scraper, collected, and pelleted at 900 3 g for 5 min at 4 8 ...900 3 g for ... See full document
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