[PDF] Top 20 Rapid Screening and Identification of Methicillin Resistant Staphylococcus aureus from Clinical Samples by Selective Broth and Real Time PCR Assay

... With the application of this method, most of the negative samples (258 of 289 [89.3%]) can be identified the next morn- ing after sampling, so the time to obtain the negative result can be reduced to 16 to ... See full document

... multiplex PCR–enzyme-linked immunosorbent assay that detects a species-specific genetic element of ...S. aureus and the mecA ...plus PCR module) also allow the de- tection of mupirocin ... See full document

... these rapid tests minimizes the time of detection of MRSA from 48 - 72 [24] to 2 - 5 h [25] ...[26]. Clinical evalua- tion data have shown that MRSA can thus be detected with very high ...(S. ... See full document

... appropriate assay becomes more ...and time-consuming, and it is hard to exclude confounding variables, even when assays are evaluated at the same ...the clinical trial, it is possible that less ... See full document

... the PCR assay by using nasal ...obtained from nine volunteers (2 nasal swabs/volunteer) who were not colonized by ...after samples were ...The identification of suspicious ... See full document

... (12). Rapid diagnostic assays, such as the Xpert MRSA assay (Cepheid, Sunnyvale, CA, USA) and the GeneOhm MRSA assay (BD Diagnostics, San Diego, CA, USA), are based on the principles of Huletsky et ... See full document

... multiplexed real-time qPCR techniques (19, 26) combining accurate identification with lim- its of detection close to a single gene copy/sample provided a significant technological advantage for the ... See full document

... MRSA screening by broth culture. Swabs collected at the same time from each individual patient were cultured in a single tube of selective broth consisting of brain heart ... See full document

... in clinical samples should have high sensitivity and specificity and, most importantly, the result should be available within a short period of ...for rapid detection of ... See full document

... mortality from MRSA bacteremia, it is crucial that new methods and good parameters for the monitoring of MRSA bacteremia be developed (3, 6, 11, 13, 14, ...the time-consuming processes of blood culture and ... See full document

... control. Methicillin resis- tance in Staphylococcus is mainly mediated by the over- production of PBP2a, an additional modified penicillin binding protein (PBP) with low affinity for β-lactam an- ... See full document

... representative clinical MRSA isolates (n ⫽ 92), identified during 1987 to 2004 in O ¨ rebro County (population approximately 280,000 inhabitants) were ...soy broth) at the Department of Clinical ... See full document

... highly resistant subpopulations of methicillin-resistant Staphylococcus aureus (MRSA) that are most likely responsible for the historic failure of the ␤-lactam family of antibiotics as ... See full document

... The ability of some microorganisms to produce biofilm is another global public health concern. Biofilms are biological communities with a high degree of organization, in which microorganisms form structured, coordinated ... See full document

... isolates from an in- tensive care unit in Hyderabad, southern India, by using the disc diffusion method ...obtained from buffalo nasal and skin samples by using the disc diffusion method ... See full document

... The clinical utility of real-time PCR screening assays for methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) colonization is constrained by the ... See full document

... transferred from patients to patients, patients to health workers or health workers to health workers or health workers to ...in clinical samples and fomites to validated the claims of CDC and other ... See full document

... Staphylococcus aureus is an important human pathogen frequently detected in hospital community and has emerged as an important health concern in human ...medicine. Identification of S. aureus ... See full document

... contact time of as low as seven seconds for a chlorhexidine-based surgical cleaning product during pre-operative hand scrubbing, despite the labelled recommendation being at least two minutes (Anderson et ... See full document

... Clinical specimens were inoculated onto blood agar base (Oxoid, Basingstoke, Hampshire, England) to which 5 % sheep blood was added and mannitol salt agar (Oxoid, Basingstoke, Hampshire, England) by using ... See full document