[PDF] Top 20 Second-strand synthesis is a rate-limiting step for efficient transduction by recombinant adeno-associated virus vectors.
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Second-strand synthesis is a rate-limiting step for efficient transduction by recombinant adeno-associated virus vectors.
... the Recombinant DNA Advisory Committee and the Food and Drug ...vector transduction can be improved dramatically in cultured cells by the physical and chemical manipulations described above ...AAV ... See full document
9
Intracellular Viral Processing, Not Single-Stranded DNA Accumulation, Is Crucial for Recombinant Adeno-Associated Virus Transduction
... AAV transduction. The enhanced AAV transduction by genotoxic agents may actually help shift the balance of degradation to ...need second-strand DNA synthesis for RNA ...AAV-CMV-EGFP ... See full document
9
Overexpression of Cyclin A Inhibits Augmentation of Recombinant Adeno-Associated Virus Transduction by the Adenovirus E4orf6 Protein
... prevents second-strand synthesis ...rAAV transduction in human cells in vitro and mu- rine cells in vivo ...rAAV transduction, such as E4orf6 expression or hydroxyurea, result in ... See full document
10
Impaired Intracellular Trafficking of Adeno-Associated Virus Type 2 Vectors Limits Efficient Transduction of Murine Fibroblasts
... Adeno-associated virus type 2 (AAV) is a nonpathogenic human parvovirus which requires coinfection with a helper virus, such as adenovirus, for its optimal replication ...helper virus, ... See full document
5
Identification and Validation of Small Molecules That Enhance Recombinant Adeno-associated Virus Transduction following High-Throughput Screens
... fAAV transduction at the same vector ...scAAV transduction, but the magnitude of the effect was signifi- cantly reduced ...the step of second-strand (ss) ...in transduction ... See full document
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Infectious Entry Pathway of Adeno-Associated Virus and Adeno-Associated Virus Vectors
... of adeno- virus, implying that AAV alone is capable of penetration into the cytosol, it was of interest to determine synergism or com- petition of endosomal escape following adenovirus and AAV ...enhance ... See full document
9
Adeno-Associated Virus Capsid Proteins May Play a Role in Transcription and Second-Strand Synthesis of Recombinant Genomes
... We note that there was a discrepancy between the confocal imaging results and the cell fractionation data. Confocal imaging using A20 antibody suggested that Y704A and E563A capsids were defective for entering the ... See full document
9
Unrestricted Hepatocyte Transduction with Adeno-Associated Virus Serotype 8 Vectors in Mice
... block second-strand synthesis (43), are downregulated or ...by second- strand synthesis (11, 12) precludes efficient ...The second model involves an upstream ... See full document
11
Conjugate-Based Targeting of Recombinant Adeno-Associated Virus Type 2 Vectors by Using Avidin-Linked Ligands
... of transduction enhancements in a tar- get cell-specific manner, based on the results obtained, it is apparent that under the biotinylation conditions used, the modified rAAV-2 still possesses tropism for the ... See full document
8
Recruitment of Single-Stranded Recombinant Adeno-Associated Virus Vector Genomes and Intermolecular Recombination Are Responsible for Stable Transduction of Liver In Vivo
... Recombinant adeno-associated virus (rAAV) vectors stably transduce hepatocytes in experimental ...rAAV vectors in vivo, single-stranded (ss) rAAV genomes become dou- ble stranded ... See full document
13
Impact of Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors
... the transduction of the outer retina by Ivt-delivered AAVs, the first of which is dilution ...vector. Second, the vitreoretinal junction, composed primar- ily of the inner limiting membrane (ILM), a ... See full document
17
Biodistribution and Safety Profile of Recombinant Adeno-Associated Virus Serotype 6 Vectors following Intravenous Delivery
... scription levels for the cytokines/chemokines interleukin-1  (IL-1  ), IL-6, tumor necrosis factor alpha (TNF- ␣ ), macro- phage chemoattractant protein 1 (MCP-1), and IP-10 after delivery. We chose the genes for these ... See full document
5
Extrachromosomal Recombinant Adeno-Associated Virus Vector Genomes Are Primarily Responsible for Stable Liver Transduction In Vivo
... We have previously suggested that the majority of rAAV genomes integrate as head-to-tail concatemers into chromo- somes in mouse hepatocytes in vivo after portal vein injection of vector (23). This was based on finding ... See full document
8
Recombinant adeno associated virus vectors induce functionally impaired transgene product–specific CD8+ T cells in mice
... This work was supported by NIH/NIAID grants PO1 A1055018-02 and PO1 HL078810-01A1. The authors wish to acknowledge Wynetta Giles-Davis for her assistance with cell culture work; Ann Cun and Lauren DiMenna for their ... See full document
14
C-Reactive Protein (CRP) Is Essential for Efficient Systemic Transduction of Recombinant Adeno-Associated Virus Vector 1 (rAAV-1) and rAAV-6 in Mice
... the recombinant adeno-associated virus (rAAV) vectors often requires widespread distribution of the vector, and in this case, systemic delivery is the optimal route of ...the ... See full document
8
Sex significantly influences transduction of murine liver by recombinant adeno-associated viral vectors through an androgen-dependent pathway.
... B6.CB17-Prkdc-SzJ mice (Figure 5B) resulted in 6-fold higher levels of hFIX in males (166 ⫾ 3 ng/mL) compared with female mice (26 ⫾ 3 ng/mL). Oophorectomy performed either before or after rAAV vector infusion did not ... See full document
9
Complement Is an Essential Component of the Immune Response to Adeno-Associated Virus Vectors
... is associated with iC3b in ...vaccinia virus-en- coded complement control protein (VCP) binds to C3b and cleaves it by acting itself as a cofactor for factor I (46, ...a virus only recently (10). ... See full document
14
Efficient Generation and Amplification of High-Capacity Adeno-Associated Virus/Adenovirus Hybrid Vectors
... Characterization of PER.tTA.Cre cell lines. Bacteriophage P1 34-bp loxP sequences are the targets for dimers of the site-specific 38-kDa bacteriophage P1 Cre recombinase. When two loxP sites are in a directly repeated ... See full document
11
Mechanistic Insights into the Enhancement of Adeno-Associated Virus Transduction by Proteasome Inhibitors
... a second-genera- tion PI, relies on this amino group to form a morpholino, cova- lently inhibiting cleavage (32), and so cannot inhibit other pro- teases (33, ...rAAV transduction and addressing the ... See full document
7
Repeated Delivery of Adeno-Associated Virus Vectors to the Rabbit Airway
... Of the five well-characterized AAV serotypes, AAV1 to AAV5, 50 to 80% of adults and children over the age of 10 are seropositive to AAV by complement fixation or neutralization assays, indicating that natural AAV ... See full document
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